HIGH FREQUENCY SOMATIC EMBRYOGENESIS IN HYPERICUM PERFORATUM

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Rezaei Nazari Mahmonir; Miri Seyed Mehdi; Ghazijahani Noushin

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Seminar Paper

Paper Information

Seminar: INTERNATIONAL CONFERENCE ON RESEARCH IN SCIENCE AND TECHNOLOGY
Year:2016 | Issue:3

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Title

HIGH FREQUENCY SOMATIC EMBRYOGENESIS IN HYPERICUM PERFORATUM

Author(s)

Rezaei Nazari Mahmonir | Miri Seyed Mehdi | Ghazijahani Noushin | Issue Writer Certificate

Keywords

EXPLANT

MEDIUM

HORMONE

SOMATIC EMBRYOGENESIS

Abstract

HYPERICUM PERFORATUM (ST. JOHN’S WORT) IS PERENNIAL HERB, WHICH CONSUMED WORLDWIDE AS ITS MEDICINAL PROPERTIES. IN THIS PLANT ACHIEVING HOMOGENEITY IN PLANT MATERIAL IS ONE OF THE MOST IMPORTANT BREEDING OBJECTIVES. ONE WAY TO ACHIEVE THESE GOALS IS SOMATIC EMBRYOGENESIS. IN THIS REGARDS, PETIOLE AND LEAF EXPLANTS OF ST. JOHN’S WORT WERE CULTURED IN MS MEDIUM SUPPLEMENTED WITH DIFFERENT CONCENTRATIONS OF 2, 4-D (0, 0.5, 1 AND 2 MG/L) AND KIN (0, 0.5, 1.5 AND 2MG/L) TO PRODUCED EMBRYONIC CALLUS. AFTER 4 WEEKS, THE PRODUCED CALLUS WAS TRANSFERRED TO MS MEDIUM SUPPLEMENTED WITH NAA (0, 1 AND 2 MG/L) AND BAP (0, 1 AND 2 MG/L) FOR SOMATIC EMBRYO PRODUCTION. THE RESULTS SHOWED THAT THE HIGHEST EMBRYONIC CALLUS WERE PRODUCED WITH PETIOLE EXPLANT AT MS MEDIUM SUPPLEMENTED WITH 1MG/L 2, 4-D AND 1.5 MG/L KIN. THE HIGHEST SOMATIC EMBRYOS OBTAINED WITH PETIOLE EXPLANT IN MS MEDIUM SUPPLEMENTED WITH 1MG/L NAA AND 1 MG/L BAP.

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APA: Copy

Rezaei Nazari, Mahmonir, Miri, Seyed Mehdi, & Ghazijahani, Noushin. (2016). HIGH FREQUENCY SOMATIC EMBRYOGENESIS IN HYPERICUM PERFORATUM. INTERNATIONAL CONFERENCE ON RESEARCH IN SCIENCE AND TECHNOLOGY. SID. https://sid.ir/paper/928408/en

Vancouver: Copy

Rezaei Nazari Mahmonir, Miri Seyed Mehdi, Ghazijahani Noushin. HIGH FREQUENCY SOMATIC EMBRYOGENESIS IN HYPERICUM PERFORATUM. 2016. Available from: https://sid.ir/paper/928408/en

IEEE: Copy

Mahmonir Rezaei Nazari, Seyed Mehdi Miri, and Noushin Ghazijahani, “HIGH FREQUENCY SOMATIC EMBRYOGENESIS IN HYPERICUM PERFORATUM,” presented at the INTERNATIONAL CONFERENCE ON RESEARCH IN SCIENCE AND TECHNOLOGY. 2016, [Online]. Available: https://sid.ir/paper/928408/en

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