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Information Journal Paper

Title

COMPARISON THE INJECTION EFFECTS OF GNRHA, HCG AND PITUITARY EXTRACT ON SPERMATOLOGICAL PARAMETERS IN GOLDFISH (CARASSIUS AURATUS)

Pages

  65-72

Abstract

 In this study the effects of GNRHA, HCG and PITUITARY EXTRACT injection on SPERMATOLOGICAL PARAMETERS¬ (motility duration, percentage of motile spermatozoa, spermatocrit, sperm density, milt volume, pH) and sperm quality was compared in GOLDFISH (Carassius auratus) males. Fish injected with hormonal GNRHA, HCG and PITUITARY EXTRACT with 10mg kg-1, 1500 IU/Kg-1, 3mg/Kg-1 respectively. There were a highly significant difference of seminal plasma pH among treatments (P<0.01), as the highest value of pH observed in treatments GNRHA and PITUITARY EXTRACT (9.26±0.30, 8.36±0.20) respectively. There were a highly significant difference for percentage of motile spermatozoa among treatments (P<0.01) as the highest value percentage of motile spermatozoa observed in treatments GNRHA and PITUITARY EXTRACT (85.07±3.20, 90.60±9.56) respectively, but there was no significant difference (P>0.05) for motility duration among treatments. There was a significant difference of spermatocrit among treatments (P<0.05), as the highest value of spermatocrit observed in treatment of PITUITARY EXTRACT (59.90±10.96). Likewise there were significant difference of sperm density among treatments (p<0.01) as the highest value of sperm density observed in treatment of PITUITARY EXTRACT (12.50×109 spermatozoa/ml semen). There was a significant difference about milt volume among treatments (p<0.01) as the highest value of milt volume observed in treatments GNRHA and PITUITARY EXTRACT (1.26±0.40, 1.05±0.13) respectively. The present study demonstrated that hormonal GNRHA and PITUITARY EXTRACT more effective on SPERMATOLOGICAL PARAMETERS compared with HCG treatment groups.

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