In Silico Analysis of the Effect of Scrophularia striata Linalool on VacA Protein of Helicobacter Pylori

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FAZELI NASAB B.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Ilam University of Medical Sciences Year:2021 | Volume:29 | Issue:1 Page(s): 50-64

Download Full-Text

Persian Verion

View:

296

Download:

Cites:

Information Journal Paper

Title

In Silico Analysis of the Effect of Scrophularia striata Linalool on VacA Protein of Helicobacter Pylori

Author(s)

FAZELI NASAB B. | Issue Writer Certificate

Keywords

Gastric cancer

Molecular docking

Scrophularia striata

VacA protein

Abstract

Introduction: Some plants, such as Scrophularia striata, has been traditionally used for the treatment of infection and ulcer among people living in Zagros District. Previous studies revealed the beneficial effect of hydroalcoholic and water extract of Scrophularia striata on ethanolic induced oxidative stress and stomach ulcer in rats. This study aimed to conduct a bioinformatics analysis to investigate the effect of linalool on the prevention of Helicobacter Pylori VacA protein activity. Materials & Methods: DNA and protein sequences of linalool of Arabidopsis thaliana and VacA gene sequence of Helicobacter pylori were obtained from the NCBI. The alignment of DNA and protein sequence of VacA and linalool was achieved by Clustalw 2 software. Furthermore, all conserved motif and domains were searched using the MEME online software. The phylogenetic tree was drawn by Mega 5 software. The ExpasyPred3D Swiss-Model, and Galaxy Web software were used to predict the third structure and Molecular docking based on homology. Findings: Linalool protein sequences in all plants have three motifs (100-300 amino acids) based on the NCBI. The VacA protein has three types of the second cytoplasm, membrane, and kinase domain. The best Molecular docking model was obtained based on the highest level of interaction (21. 0) and accuracy (0. 133) between linalool and VacA protein. The results showed that some molecular groups, such as aliphatic, polar, and hydrophilic, were found in both VacA and linalool protein structure. The presence of aliphatic and hydrophilic groups in VacA protein could give it the ability to pass through the cells by lymphocyte cells and caused pathogenesis if they could escape from degradation. On the other hand, the presence of these groups in linalool could block the VacA protein effect. Discussions & Conclusions: Bioinformatics analysis showed that linalool eliminated the effects of Helicobacter pylori. Furthermore, the results of this study could be used as a scientific basis for future studies to evaluate the effect of each ingredient of Scrophularia striata, especially linalool, on Helicobacter pylori.

Cites

Investigating the Antimicrobial Effects of Glycyrrhiza glabra and Salvia officinalis Ethanolic Extract Against Helicobacter pylori

References

Cite

APA: Copy

FAZELI NASAB, B.. (2021). In Silico Analysis of the Effect of Scrophularia striata Linalool on VacA Protein of Helicobacter Pylori. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 29(1 ), 50-64. SID. https://sid.ir/paper/950672/en

Vancouver: Copy

FAZELI NASAB B.. In Silico Analysis of the Effect of Scrophularia striata Linalool on VacA Protein of Helicobacter Pylori. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2021;29(1 ):50-64. Available from: https://sid.ir/paper/950672/en

IEEE: Copy

B. FAZELI NASAB, “In Silico Analysis of the Effect of Scrophularia striata Linalool on VacA Protein of Helicobacter Pylori,” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 29, no. 1 , pp. 50–64, 2021, [Online]. Available: https://sid.ir/paper/950672/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops