THE STUDY OF ION EXCHANGE CHROMATOGRAPHY AS A FRAGMENTATION AND AGGREGATION OF IMMUNOGLOBULIN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HASHEMI M.; VARASTEH A.R.; FAZLI BAZAZ B.S.; MOGHADAM MALIHEH; BAHRAMI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Birjand University of Medical Sciences Year:2002 | Volume:9 | Issue:1 (14) Page(s): 1-4

Download Full-Text

Persian Verion

View:

1,104

Download:

Cites:

Information Journal Paper

Title

THE STUDY OF ION EXCHANGE CHROMATOGRAPHY AS A FRAGMENTATION AND AGGREGATION OF IMMUNOGLOBULIN

Author(s)

Keywords

INTRAVENOUS IMMUNOGLOBULINQ3

ANTI-DQ4

ION EXCHANGE CHROMATOGRAPHYQ4

Abstract

Introduction and aim: Most immunoglobulin preparations are produced by the cohn cold ethanol precipitation technique and are recommended for intramuscular administration only. The immunoglobulin prepared by ION EXCHANGE CHROMATOGRAPHY is suitable for intravenous injection because of low aggregation and anticomplemantary activity.Material and methods: Amount of aggregation and fragmentation (gel filtration), IgA (LC SRID) and parity (SDS-PAGE and acetate cellulose electrophoresis) was assayed in Anti-RhD prepared by ION EXCHANGE CHROMATOGRAPHY and compared with Cohn preparation.Results: The result indicated that IgG Anti-RhD prepared by ION EXCHANGE CHROMATOGRAPHY consisted principally of the IgG monomer (>95%) with traces of aggregation and fragmentation (<3%) (In Cohn method: 60% IgG monomer and >20% agg. and frag.).Conclusion: Then, ION EXCHANGE CHROMATOGRAPHY is a method for producing high purity of IgG Anti-RhD, which has low aggregation and IgA and hence intravenouses tolerate.This method could be easily modified for another intravenous IgG preparations.

Cites

No record.

References

No record.

Cite

APA: Copy

HASHEMI, M., VARASTEH, A.R., FAZLI BAZAZ, B.S., MOGHADAM, MALIHEH, & BAHRAMI, A.. (2002). THE STUDY OF ION EXCHANGE CHROMATOGRAPHY AS A FRAGMENTATION AND AGGREGATION OF IMMUNOGLOBULIN. JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES, 9(1 (14)), 1-4. SID. https://sid.ir/paper/95347/en

Vancouver: Copy

HASHEMI M., VARASTEH A.R., FAZLI BAZAZ B.S., MOGHADAM MALIHEH, BAHRAMI A.. THE STUDY OF ION EXCHANGE CHROMATOGRAPHY AS A FRAGMENTATION AND AGGREGATION OF IMMUNOGLOBULIN. JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2002;9(1 (14)):1-4. Available from: https://sid.ir/paper/95347/en

IEEE: Copy

M. HASHEMI, A.R. VARASTEH, B.S. FAZLI BAZAZ, MALIHEH MOGHADAM, and A. BAHRAMI, “THE STUDY OF ION EXCHANGE CHROMATOGRAPHY AS A FRAGMENTATION AND AGGREGATION OF IMMUNOGLOBULIN,” JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES, vol. 9, no. 1 (14), pp. 1–4, 2002, [Online]. Available: https://sid.ir/paper/95347/en

Related Journal Papers