Cloning and Expression of Serratia Marcescens Prodigiosin Gene in Ecoli XL1blue

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AKBARI MARYAM; AMINI KUMARSS

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Jundishapur Scientific Medical Journal Year:2021 | Volume:20 | Issue:2 Page(s): 102-111

Download Full-Text

Persian Verion

View:

302

Download:

Cites:

Information Journal Paper

Title

Cloning and Expression of Serratia Marcescens Prodigiosin Gene in Ecoli XL1blue

Author(s)

AKBARI MARYAM | AMINI KUMARSS | Issue Writer Certificate

Keywords

Serratia marcescens

Prodigiosin

Cloning

Bacterial pigments

Real Time PCR

Abstract

Background and Objectives: Serratia is a gram-negative bacterium. The pigmentation property of Serratia marcescens is used as a marker of dust particles in the environment and in the hospital. Today biopigments are also widely used in the manufacture and production of pharmaceutical products. Prodigiosin is a promising drug due to its reported properties of antifungal immunosuppressive and antiproliferative activities. In the present study, Cloning of pig gene-isolated from Serratia marcescens in Ecoli XL1blue was performed. Subjects and Methods 60 Samples were taken from clinical sources of patients hospitalized with urinary tract infections in Saveh Hospitals. Serratia marcescens were identified and isolated by different tests. The pig gene was cloned by T-A Cloning using PTG-19 vector into the Escherichia coli XL1blue as host. Expression of cloned gene in recombinant colonies was evaluated by Real Time PCR. The phylogenetic tree was plotted using clustalX and Mega5 software Results Screening of samples identified 12 isolates of Serratia marcescens from then 4 isolates had pig gene. Expression of Pig gene in Escherichia coli XL1blue was confirmed by Real-Tima PCR. As a result of phylogenetic studies, some close relatives of serratia have been identified as candidates for further studies Conclusion Serratia marcescens can be considered as a rich source of pigments with many applications and can be used as indigenous strains to produce Prodigiosin.

Cites

No record.

References

No record.

Cite

APA: Copy

AKBARI, MARYAM, & AMINI, KUMARSS. (2021). Cloning and Expression of Serratia Marcescens Prodigiosin Gene in Ecoli XL1blue. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, 20(2 ), 102-111. SID. https://sid.ir/paper/954427/en

Vancouver: Copy

AKBARI MARYAM, AMINI KUMARSS. Cloning and Expression of Serratia Marcescens Prodigiosin Gene in Ecoli XL1blue. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL[Internet]. 2021;20(2 ):102-111. Available from: https://sid.ir/paper/954427/en

IEEE: Copy

MARYAM AKBARI, and KUMARSS AMINI, “Cloning and Expression of Serratia Marcescens Prodigiosin Gene in Ecoli XL1blue,” JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, vol. 20, no. 2 , pp. 102–111, 2021, [Online]. Available: https://sid.ir/paper/954427/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops