Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold

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KHAZAEI SAMANEH; SOLEIMANI MASOUD; AHMADI TAFTI SEYED HOSSEIN; HOJATI ZOHREH

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Journal: Qom University of Medical Sciences Journal Year:2021 | Volume:15 | Issue:5 Page(s): 368-377

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Information Journal Paper

Title

Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold

Author(s)

KHAZAEI SAMANEH | SOLEIMANI MASOUD | AHMADI TAFTI SEYED HOSSEIN | HOJATI ZOHREH | Issue Writer Certificate

Keywords

Cardiovascular diseases

Mesenchymal stem cells

miR-1

Organ culture techniques

Cell differentiation

Abstract

Background and Objectives Cardiovascular disease is one of the leading causes of death worldwide. Mesenchymal stem cells (MSCs) are one of the most common sources of cell-based therapies in heart regeneration. There are several approaches to differentiate MSCs into cardiac-like cells, such as genetic modification. In addition, using of 3D culture, such as hydrogels, increases the efficiency of differentiation. Methods In the present study, lentiviruses containing microRNA 1 (miR-1) and myocardium (Myocd) were co-transducted to mouse adipose-derived MSCs. Three days after, transduced MSCs were transferred to a hydrogel containing chitosan and collagen. After 21 days, the differentiation of encapsulated cells was evaluated. In this regard, the expression of cardiac markers such as NK2 homeobox 5 (Nkx2-5), GATA binding protein 4 (Gata4) and troponin T type 2 (Tnnt2) at the level of gene and protein were investigated. Results The results of real-time quantitative polymerase chain reaction (qRT-PCR) and immunocytochemistry showed that co-induction of miR-1 and Myocd in MSCs followed by transfer to composite hydrogel increased the expression of cardiac markers. Conclusion The use of 3D culture such as chitosan/collagen hydrogel improves the differentiation of MSCs and subsequently obtains more mature cells for use in cell-based regenerative medicine

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APA: Copy

KHAZAEI, SAMANEH, SOLEIMANI, MASOUD, AHMADI TAFTI, SEYED HOSSEIN, & HOJATI, ZOHREH. (2021). Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 15(5 ), 368-377. SID. https://sid.ir/paper/959809/en

Vancouver: Copy

KHAZAEI SAMANEH, SOLEIMANI MASOUD, AHMADI TAFTI SEYED HOSSEIN, HOJATI ZOHREH. Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL[Internet]. 2021;15(5 ):368-377. Available from: https://sid.ir/paper/959809/en

IEEE: Copy

SAMANEH KHAZAEI, MASOUD SOLEIMANI, SEYED HOSSEIN AHMADI TAFTI, and ZOHREH HOJATI, “Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold,” QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, vol. 15, no. 5 , pp. 368–377, 2021, [Online]. Available: https://sid.ir/paper/959809/en

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