DNA barcoding of Ziziphus jujuba Mill. in Iran using chloroplast genes (rbcL and matK)

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MOUDI M.; Mousavi Kouhi S.M.; Ghollasi Mood Sh.; Auobi A.

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Journal Paper

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Journal: Journal of Plant Production Research Year:2021 | Volume:28 | Issue:2 Page(s): 115-130

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Title

DNA barcoding of Ziziphus jujuba Mill. in Iran using chloroplast genes (rbcL and matK)

Author(s)

MOUDI M. | Mousavi Kouhi S.M. | Ghollasi Mood Sh. | Auobi A. | Issue Writer Certificate

Keywords

Choloroplast genes

DNA barcoding

Jujuba (Ziziphus jujuba Mill.)

matK

rbcL

Abstract

Background and Objectives: Jujube (Ziziphus jujuba Mill. ) is one of the most important medicinal plants belong to the Rhamnaceae family. It is important in the pharmaceutical industry. Genetic diversity of different species of Z. jujuba in Iran in 2007 and 2006 classified the sex of 19 species by simultaneous analysis of morphological traits and molecular methods. The main purpose of this study is DNA barcoding of different ecotypes of Z. jujuba in South Khorasan, Iran using two chloroplast genes (rbcL and matK). Materials and Methods: The 25 numbers of the ecotypes of this species from 6 different provinces of Iran were planted in South Khorasan, were assessed. Two close relatives of the same family (Sangoisorba sp., Rosa sp. ) were also used as external groups. Purification (protein and polysaccharide refining) was performed using the manual method. The gel was then stained with ethidium bromide and the DNA quality was estimated using the agarose gel electrophoresis results. The chloroplast genes from the DNA extracted were amplified using the PCR technique. All sequences obtained from forward and reverse reading in this study to produce the final sequence using appropriate software were assembled. Results: The DNA barcode of each species was performed for fast, accurate and automatic identification of the species and all the sequences obtained from this study were sent to the NCBI (National Center for Biotechnology Information) and submitted. The results show that there is the greatest genetic distance between jujube ecotypes and two samples from the outgroup and no significant genetic diversity between different jujube ecotypes and morphological differences are due to ecological conditions. However, the analysis of gene combination data provided more informational insights than the separate analysis of sequence data. According to the results, the highest diversity was observed in the ecotypes of South Khorasan. Conclusion: The results of pairwise distance and haplotype networks showed the most variety among different ecotypes belonged to the different areas of South Khorasan province. Also, Soth Khorasan can be considered as the origin of this species in Iran. Previous studies have fully confirmed the results of this study. However, given the economic importance of jujube plants in the world, Iran and especially South Khorasan, it is recommended to use more samples and more genes, especially nuclear genes, to study more closely the genetic similarities and differences of these ecotypes.

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APA: Copy

MOUDI, M., Mousavi Kouhi, S.M., Ghollasi Mood, Sh., & Auobi, A.. (2021). DNA barcoding of Ziziphus jujuba Mill. in Iran using chloroplast genes (rbcL and matK). JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), 28(2 ), 115-130. SID. https://sid.ir/paper/960669/en

Vancouver: Copy

MOUDI M., Mousavi Kouhi S.M., Ghollasi Mood Sh., Auobi A.. DNA barcoding of Ziziphus jujuba Mill. in Iran using chloroplast genes (rbcL and matK). JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES)[Internet]. 2021;28(2 ):115-130. Available from: https://sid.ir/paper/960669/en

IEEE: Copy

M. MOUDI, S.M. Mousavi Kouhi, Sh. Ghollasi Mood, and A. Auobi, “DNA barcoding of Ziziphus jujuba Mill. in Iran using chloroplast genes (rbcL and matK),” JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), vol. 28, no. 2 , pp. 115–130, 2021, [Online]. Available: https://sid.ir/paper/960669/en

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