مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

264
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Gene transfer biotechnology in zebrafish as a gene transfer research model in farmed aquatic animals

Pages

  47-55

Abstract

 The polymerase chain reaction (PCR) was applied to create the colored transgenic ornamental zebrafish with the specific primers designed for muscle promoter of two genes and genes fragments cloned in Tol2 vector containing GFP gene in the ORF region. The 2000 bp and 2300 bp fragments of MYLZ2 and CKMB promoters' genes respectively were amplified by PCR, cloned into Tol2 plasmid expression vector containing SalI restriction enzyme site and transformed into E. coli Dh5α . Positive clones were tested for having fragments by colony PCR with anchor primers and subcultured for extracting recombinant plasmid miniprep. The extracted recombinant plasmid was tested with SalI enzyme digestion and sequenced with universal primers. Positive recombinant plasmids were applied for injection with transcript produced synthetically in the lab into zebrafish one-cell embryo. In this study, 2000 bp and 2300 bp fragments of MYLZ2 and CKMB promoters, respectively, were cloned in an expression vector Tol2 and confirmed by PCR, sequencing and enzymatic digestion. Also, recombinant plasmid was subcultured and purified for injection with a synthetic transcript. One-cell embryos injected by coinjection materials (recombinant plasmid and transcript) were tested by fluorescence microscope for positive GFP expression, where a few offspring of F0 generation were positive for GFP expression in their muscles. Recombinant plasmids with muscle-specific promoters (MYLZ2 and CKMB) were able to induce expression of GFP in some F0 embryos in a transient form and could be applied for consolidation in the next fish generation.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    JAMSHIDI, SHIRIN, & Banan, shkan. (2022). Gene transfer biotechnology in zebrafish as a gene transfer research model in farmed aquatic animals. JOURNAL OF AQUACULTURE DEVELOPMENT, 15(4 ), 47-55. SID. https://sid.ir/paper/963181/en

    Vancouver: Copy

    JAMSHIDI SHIRIN, Banan shkan. Gene transfer biotechnology in zebrafish as a gene transfer research model in farmed aquatic animals. JOURNAL OF AQUACULTURE DEVELOPMENT[Internet]. 2022;15(4 ):47-55. Available from: https://sid.ir/paper/963181/en

    IEEE: Copy

    SHIRIN JAMSHIDI, and shkan Banan, “Gene transfer biotechnology in zebrafish as a gene transfer research model in farmed aquatic animals,” JOURNAL OF AQUACULTURE DEVELOPMENT, vol. 15, no. 4 , pp. 47–55, 2022, [Online]. Available: https://sid.ir/paper/963181/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button