STUDY OF MORPHOLOGICAL AND PROTECTIVE CHARACTERISTICS OF BEADS OBTAINED FROM MICROENCAPSULATION OF LACTOBACILLUS ACIDOPHILUS PROBIOTIC AS A PREDOMINANT AND NATURAL FLORA IN HUMAN GUT

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POURJAFAR H.; MIRZAEI H.; GHASEMNEZHAD R.; HOMAYOUNI RAD A.

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Journal Paper

Paper Information

Journal: Annals of Military and Health Sciences Research Year:2012 | Volume:9 | Issue:4 (SERIAL NUMBER 36) Page(s): 233-240

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Title

STUDY OF MORPHOLOGICAL AND PROTECTIVE CHARACTERISTICS OF BEADS OBTAINED FROM MICROENCAPSULATION OF LACTOBACILLUS ACIDOPHILUS PROBIOTIC AS A PREDOMINANT AND NATURAL FLORA IN HUMAN GUT

Author(s)

POURJAFAR H. | MIRZAEI H. | GHASEMNEZHAD R. | HOMAYOUNI RAD A. | Issue Writer Certificate

Keywords

MICROENCAPSULATIONQ2

PROBIOTICQ2

LACTOBACILLUS ACIDOPHILUSQ3

PREDOMINANT GUT FLORAQ2

Abstract

Background: LACTOBACILLUS ACIDOPHILUS as a PROBIOTIC has more useful effects and low viability of this bacterium and other PROBIOTICs under hard acidic-bile conditions of digestive system and food products, have encouraged researchers to find methods to solve this problem. MICROENCAPSULATION as one of the newest methods has noticeable effects in this field. The aim of this study was to evaluate the morphological and protective characteristics of beads obtained from MICROENCAPSULATION of LACTOBACILLUS ACIDOPHILUS.Materials and methods: After activation of starter culture of la5 in MRS-broth medium, in order to purification bacteria was used centrifuge device with speed of 10000rpm for 10 minutes. MICROENCAPSULATION was done by calcium alginate and resistant starch with extrusion method. Morphology of beads was evaluated by optical microscope. The study of beads stability was done for 12 hours and the study of survival microencapsulated bacteria was done for 120 minutes within hydrochloric acid, phosphate buffer (0.1 mol) and digestive powder inclusion solution.Results: A morphological characteristics of beads was indicated by optical microscope. These results showed that the strength of beads in various environments is different and in this regard physical tension is very important. Under unfavorable environmental conditions, MICROENCAPSULATION with calcium alginate and resistant starch was played an important role in the protection of LACTOBACILLUS ACIDOPHILUS. The survival rate of microencapsulated bacteria in all conditions was significantly higher than free bacteria (P<0/05).Conclusion: Morphological characteristics and strength of beads had great importance in theirs protective function of PROBIOTIC bacteria. MICROENCAPSULATION with calcium alginate and resistant starch plays an important role in increasing the rate of LACTOBACILLUS ACIDOPHILUS survival.

Cites

PRODUCING OF CHOCOLATE MILK CONTAINING MICROENCAPSULATED PROBIOTIC BACTERIA AS A FUNCTIONAL FOOD FOR USE IN TIME OF WAR

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APA: Copy

POURJAFAR, H., MIRZAEI, H., GHASEMNEZHAD, R., & HOMAYOUNI RAD, A.. (2012). STUDY OF MORPHOLOGICAL AND PROTECTIVE CHARACTERISTICS OF BEADS OBTAINED FROM MICROENCAPSULATION OF LACTOBACILLUS ACIDOPHILUS PROBIOTIC AS A PREDOMINANT AND NATURAL FLORA IN HUMAN GUT. ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH, 9(4 (SERIAL NUMBER 36)), 233-240. SID. https://sid.ir/paper/96528/en

Vancouver: Copy

POURJAFAR H., MIRZAEI H., GHASEMNEZHAD R., HOMAYOUNI RAD A.. STUDY OF MORPHOLOGICAL AND PROTECTIVE CHARACTERISTICS OF BEADS OBTAINED FROM MICROENCAPSULATION OF LACTOBACILLUS ACIDOPHILUS PROBIOTIC AS A PREDOMINANT AND NATURAL FLORA IN HUMAN GUT. ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH[Internet]. 2012;9(4 (SERIAL NUMBER 36)):233-240. Available from: https://sid.ir/paper/96528/en

IEEE: Copy

H. POURJAFAR, H. MIRZAEI, R. GHASEMNEZHAD, and A. HOMAYOUNI RAD, “STUDY OF MORPHOLOGICAL AND PROTECTIVE CHARACTERISTICS OF BEADS OBTAINED FROM MICROENCAPSULATION OF LACTOBACILLUS ACIDOPHILUS PROBIOTIC AS A PREDOMINANT AND NATURAL FLORA IN HUMAN GUT,” ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH, vol. 9, no. 4 (SERIAL NUMBER 36), pp. 233–240, 2012, [Online]. Available: https://sid.ir/paper/96528/en

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