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Information Journal Paper

Title

The Evaluation of 16S rRNA, rpoB and hsp65 Genes in Accurate Identification of Nontuberculous Mycobacteria (NTM) Species

Pages

  42-50

Abstract

 Background and objective: Nontuberculous mycobacteria (NTM) are a large family of acid-fast bacteria that are widely distributed in the environment. Although most NTM species are saprophytic, about 30% of these species are associated with human diseases. NTM and Mycobacterium tuberculosis complex have common microbiological characteristics, such as induce similar immune response and same clinical manifestations. However, disease caused by NTM is a diagnostic challenge for physicians, because it cannot readily be distinguished from tuberculosis on the basis of clinical history, tuberculin skin test results, radiological patterns, and initial laboratory reports. Therefore, accurate detection of NTM is necessary due to different therapeutic strategies and should be considered seriously. Materials and methods: In this study, 120 water samples were collected for isolation of NTM species, and cultured in a Lowenstein-Jensen medium after decontamination and processing. Then, accuracy and sensitivity of the three conserved genes (16S rRNA, rpoB and hsp65), were evaluated in Identification of NTM species isolated. Result: In this study, 87 NTM colonies were isolated from water samples. The results of the sequence analysis of the three conserved genes showed that the hsp65 gene compared to other two genes has a high degree of accuracy and sensitivity. Conclusion: Unlike hsp65 gene, partial sequence analysis of the 16S rRNA and rpoB genes does not seem to be sufficient for recognizing NTM species.

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    APA: Copy

    Roshdi Maleki, Mehdi. (2020). The Evaluation of 16S rRNA, rpoB and hsp65 Genes in Accurate Identification of Nontuberculous Mycobacteria (NTM) Species. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 25(91 ), 42-50. SID. https://sid.ir/paper/965531/en

    Vancouver: Copy

    Roshdi Maleki Mehdi. The Evaluation of 16S rRNA, rpoB and hsp65 Genes in Accurate Identification of Nontuberculous Mycobacteria (NTM) Species. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2020;25(91 ):42-50. Available from: https://sid.ir/paper/965531/en

    IEEE: Copy

    Mehdi Roshdi Maleki, “The Evaluation of 16S rRNA, rpoB and hsp65 Genes in Accurate Identification of Nontuberculous Mycobacteria (NTM) Species,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 25, no. 91 , pp. 42–50, 2020, [Online]. Available: https://sid.ir/paper/965531/en

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