STUDY OF BIOFILM FORMATION BY PSEUDOMONAS AERUGINOSA USING MODIFIED MICROTITRE PLATE AND SCANNING ELECTRON MICROSCOPE

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MOHAMMADI MEHR M.; ABDI AALI A.

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Journal: Annals of Military and Health Sciences Research Year:2004 | Volume:2 | Issue:1 (Serial Number 5) Page(s): 295-299

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Title

STUDY OF BIOFILM FORMATION BY PSEUDOMONAS AERUGINOSA USING MODIFIED MICROTITRE PLATE AND SCANNING ELECTRON MICROSCOPE

Author(s)

MOHAMMADI MEHR M. | ABDI AALI A. | Issue Writer Certificate

Keywords

ANTIBIOTIC RESISTANCEQ2

BIOFILMQ2

PSEUDOMONAS AERUGINOSAQ2

Abstract

Background: in this study, using a modified microtitre plate and scanning electron microscope, we investigated BIOFILM production by PSEUDOMONAS AERUGINOSA BIOFILM productions are difficult to eradicate with antimicrobial treatment and up to 60% of all human infections are caused by BIOFILMs. Bacterial BIOFILMs may be found at various sites, including mucus membrane, teeth and indwelling medical devices. PSEUDOMONAS AERUGINOSA is one of the most important opportunistic bacteria and producing BIOFILM. It has been reported that BIOFILM forming bacteria are more resistance to antibiotic treatment and immunologic attack. Materials and methods: clinical isolates of Paeruginosa were collected from hospital and identified by biochemical tests. A modified microtitre plate as described by stepanovic was used to determine the BIOFILM formation capacity of Paeruginosa and measured using ELlSA reader. The bacteria BIOFILM on the Teflon sheets and cather were fixed by modified method described by Ishila. They were observed with scanning electron microscope. Results: the results demonstrated that PSEUDOMONAS AERUGINOSA strain M214 denser BIOFILM formation compared with other strain, with OD=2.89 using modified microtitre plate test. Scanning electron micrograph of bacteria on the Teflon and catheter that had been incubated with Paeruginosa M214 for 1 and 7 days were investigated. The results showed BIOFILM formation on catheter was denser that Teflon sheets. The bacteria incubated for 7 days on Teflon and catherter were covered with thick membranous and fibrous structure, unlike bacteria incubated for 1 day. Conclusion: It seems modified microtitre plate is better and easier way to use in diagnostic laboratories regard to treatment difficulties, it strongly recommend studying the BIOFILM before antibiotic administration.

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APA: Copy

MOHAMMADI MEHR, M., & ABDI AALI, A.. (2004). STUDY OF BIOFILM FORMATION BY PSEUDOMONAS AERUGINOSA USING MODIFIED MICROTITRE PLATE AND SCANNING ELECTRON MICROSCOPE. ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH, 2(1 (Serial Number 5)), 295-299. SID. https://sid.ir/paper/96623/en

Vancouver: Copy

MOHAMMADI MEHR M., ABDI AALI A.. STUDY OF BIOFILM FORMATION BY PSEUDOMONAS AERUGINOSA USING MODIFIED MICROTITRE PLATE AND SCANNING ELECTRON MICROSCOPE. ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH[Internet]. 2004;2(1 (Serial Number 5)):295-299. Available from: https://sid.ir/paper/96623/en

IEEE: Copy

M. MOHAMMADI MEHR, and A. ABDI AALI, “STUDY OF BIOFILM FORMATION BY PSEUDOMONAS AERUGINOSA USING MODIFIED MICROTITRE PLATE AND SCANNING ELECTRON MICROSCOPE,” ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH, vol. 2, no. 1 (Serial Number 5), pp. 295–299, 2004, [Online]. Available: https://sid.ir/paper/96623/en

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