STUDY THE CAPACITY OF RECOMBINANT HCV CORE+1 PROTEIN TO INDUCE IMMUNE RESPONSES IN COMBINATION WITH DIFFERENT ADJUVANTS

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BAGHBANI ARANI F.; ROOHVAND F.; AGHASADEGHI M.R.; SADAT S.N.; MOTEVALLI F.; MEMARNEJADIAN A.; AMINI S.

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Journal: Annals of Military and Health Sciences Research Year:2012 | Volume:10 | Issue:1 (SERIAL NUMBER 37) Page(s): 1-9

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Title

STUDY THE CAPACITY OF RECOMBINANT HCV CORE+1 PROTEIN TO INDUCE IMMUNE RESPONSES IN COMBINATION WITH DIFFERENT ADJUVANTS

Author(s)

Keywords

CORE+1Q2

HCVQ3

ADJUVANTQ2

EXPRESSIONQ2

BALB/CQ2

Abstract

Background: Hepatitis C virus (HCV) genome contains a large open reading frame coding for a polyprotein that is cleaved into ten proteins. Recently, a new protein, named core+1, has been described to be expressed through a ribosomal frame shift within the capsid-encoding sequence. To address these possibilities, core+1 was produced inE.coli and the purified protein was evaluated for the immunological properties.Material and Methods: The core+1corresponding nucleotide sequence was created by PCR-based induction of a+1 frame shift mutation within the core gene template. The amplicon was cloned into the pET-24a vector and expressed inE.coli host. The expressed protein was purified under denaturing condition and after refolding steps was characterized by SDS-PAGE and Western Blotting. The immunization potential of core+1 with various ADJUVANT (Freunds (C/IFA), Montanide ISA 206 and IMS 1312, pluronic acid (F127) and imiquimod (IMIQ) was assessed in BALB/C mice. ELISA-based assays were used to analyze the humoral immune responses.Results: The yield of E.coli -derived core+1 was 5 mg/ L of culture media and antigenicity of this protein was confirmed by western blotting. All the core+1/adjuvant formulations significantly developed the anticore+ 1 IgG responses in the immunized mice but C/IFA and ISA206 elicited the highest antibody titers. ISA 206 and IMS 1312 formulation of core+1 induced strong Th1/Th2 responses.Conclusions: Our results indicated that core+1 formulation with various ADJUVANTs may elicit the different immune response profiles (Th1/Th2). Thus core+1 might be a potential component of future HCV vaccine too.

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APA: Copy

BAGHBANI ARANI, F., ROOHVAND, F., AGHASADEGHI, M.R., SADAT, S.N., MOTEVALLI, F., MEMARNEJADIAN, A., & AMINI, S.. (2012). STUDY THE CAPACITY OF RECOMBINANT HCV CORE+1 PROTEIN TO INDUCE IMMUNE RESPONSES IN COMBINATION WITH DIFFERENT ADJUVANTS. ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH, 10(1 (SERIAL NUMBER 37)), 1-9. SID. https://sid.ir/paper/96664/en

Vancouver: Copy

BAGHBANI ARANI F., ROOHVAND F., AGHASADEGHI M.R., SADAT S.N., MOTEVALLI F., MEMARNEJADIAN A., AMINI S.. STUDY THE CAPACITY OF RECOMBINANT HCV CORE+1 PROTEIN TO INDUCE IMMUNE RESPONSES IN COMBINATION WITH DIFFERENT ADJUVANTS. ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH[Internet]. 2012;10(1 (SERIAL NUMBER 37)):1-9. Available from: https://sid.ir/paper/96664/en

IEEE: Copy

F. BAGHBANI ARANI, F. ROOHVAND, M.R. AGHASADEGHI, S.N. SADAT, F. MOTEVALLI, A. MEMARNEJADIAN, and S. AMINI, “STUDY THE CAPACITY OF RECOMBINANT HCV CORE+1 PROTEIN TO INDUCE IMMUNE RESPONSES IN COMBINATION WITH DIFFERENT ADJUVANTS,” ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH, vol. 10, no. 1 (SERIAL NUMBER 37), pp. 1–9, 2012, [Online]. Available: https://sid.ir/paper/96664/en

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