EFFECT OF CELL SIZE AND SHAPE ON ELECTRIC FIELD THRESHOLD AND CRITICAL TRANSMEMBRANE VOLTAGE FOR ELECTROPORATION

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TOUHIDI L.; POURMIR FIROUZABADI S.M.; KOTNIK T.; PUCIHAR G.; MIKLAVCIC D.

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Journal: IRANIAN JOURNAL OF MEDICAL PHYSICS Year:2009 | Volume:5 | Issue:2 (20-21) Page(s): 1-14

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Title

EFFECT OF CELL SIZE AND SHAPE ON ELECTRIC FIELD THRESHOLD AND CRITICAL TRANSMEMBRANE VOLTAGE FOR ELECTROPORATION

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Keywords

ELECTROPORATIONQ3

CELL SIZEQ3

CELL SHAPEQ3

CRITICAL TRANSMEMBRANE VOLTAGEQ3

PULSE STRENGTH THRESHOLDQ3

Abstract

Introduction: ELECTROPORATION is a technique for increasing the permeability of the cell membrane to otherwise non-permeate molecules due to an external electric field. This permeability enhancement is detectable if the induced transmembrane voltage becomes greater than a critical value which depends on the PULSE STRENGTH THRESHOLD. In this study, the variability of the electric field threshold and the minimal transmembrane voltage resulting in detectable ELECTROPORATION of the plasma membrane of spherical and irregularly shaped cells have been investigated.Materials and Methods: Spherical cells of different dimensions and attached cells of various shapes were selected. The chosen cells were exposed to 100 µs electric pulses with incrementing amplitudes. ELECTROPORATION was detected by an increase in the fluorescence caused by an influx of Ca2+ and the threshold electric field for each cell was recorded. A 3D geometrical model of each cell was constructed from its cross sectional images. Simulation using the finite element method was performed to obtain the critical induced transmembrane voltage for each individual cell.Results: The magnitudes of the electric field strength threshold and CRITICAL TRANSMEMBRANE VOLTAGE versus cell radius were obtained for spherical cells. To investigate the effects of CELL SHAPEs and orientations on the field strength threshold and critical voltage, the considered attached cells were categorized into three different groups. Field strength threshold and critical voltage was obtained for each cells and the results for the different groups were compared.Discussion and Conclusion: Size, shape and orientation of cells affect the CRITICAL TRANSMEMBRANE VOLTAGE and all these elements in turn influence the electric field threshold and, therefore, the efficiency of ELECTROPORATION.

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APA: Copy

TOUHIDI, L., POURMIR FIROUZABADI, S.M., KOTNIK, T., PUCIHAR, G., & MIKLAVCIC, D.. (2009). EFFECT OF CELL SIZE AND SHAPE ON ELECTRIC FIELD THRESHOLD AND CRITICAL TRANSMEMBRANE VOLTAGE FOR ELECTROPORATION. IRANIAN JOURNAL OF MEDICAL PHYSICS, 5(2 (20-21)), 1-14. SID. https://sid.ir/paper/96989/en

Vancouver: Copy

TOUHIDI L., POURMIR FIROUZABADI S.M., KOTNIK T., PUCIHAR G., MIKLAVCIC D.. EFFECT OF CELL SIZE AND SHAPE ON ELECTRIC FIELD THRESHOLD AND CRITICAL TRANSMEMBRANE VOLTAGE FOR ELECTROPORATION. IRANIAN JOURNAL OF MEDICAL PHYSICS[Internet]. 2009;5(2 (20-21)):1-14. Available from: https://sid.ir/paper/96989/en

IEEE: Copy

L. TOUHIDI, S.M. POURMIR FIROUZABADI, T. KOTNIK, G. PUCIHAR, and D. MIKLAVCIC, “EFFECT OF CELL SIZE AND SHAPE ON ELECTRIC FIELD THRESHOLD AND CRITICAL TRANSMEMBRANE VOLTAGE FOR ELECTROPORATION,” IRANIAN JOURNAL OF MEDICAL PHYSICS, vol. 5, no. 2 (20-21), pp. 1–14, 2009, [Online]. Available: https://sid.ir/paper/96989/en

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