COMPARISON OF PROPAGATION AND CONTINUOUS PASSAGES OF NEOSPORA CANINUM TACHYZOITES IN VERO AND TLI CELL LINES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KHORDADMEHR M.; ZEHTAB NAJAFI M.; NAMAVARI M.; KHODA KARAM TAFTI A.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2017 | Volume:13 | Issue:3 Page(s): 32-42

Download Full-Text

Persian Verion

View:

668

Download:

Cites:

Information Journal Paper

Title

COMPARISON OF PROPAGATION AND CONTINUOUS PASSAGES OF NEOSPORA CANINUM TACHYZOITES IN VERO AND TLI CELL LINES

Author(s)

KHORDADMEHR M. | ZEHTAB NAJAFI M. | NAMAVARI M. | KHODA KARAM TAFTI A.A. | Issue Writer Certificate

Keywords

NEOSPORA CANINUMQ2

CELL CULTUREQ2

VERO CELL LINEQ1

TLI CELL LINEQ1

Abstract

NEOSPORA CANINUMcauses major bovine abortions and neonatal mortality in cattle, sheep, goat and horse. Vero cells are the most frequently used cell line for culture ofNEOSPORA CANINUMbut problems are with propagation and required level of nutritional components for the media persist.In this study the tachyzoite yields of N. caninum were compared in two cell lines: Vero (African green monkey kidney) and suspension cultureTheileria lestoquardi -infected lymphoblastoid (TLI)cell lines. Then, N. caninum were continuously passaged in these cell lines for 3 months and the effect of host cells on virulence of tachyzoites was assessed by broiler chicken embryonated eggs. Inoculation was performed in the chorioallantoic (CA) liquid of the embryonated eggs with different dilutions (0.5´104, 1.0´104, 1.5´104 /ml) of tachtzoites isolated from these CELL CULTUREs. The mortality pattern and pathological changes of the dead embryos and hatched chickens were noted. Tissue samples of brain, liver and heart were examined by histopathological and detection of DNA of parasite by polymerase chain reaction (PCR). Also, consecutive sections of the tissues examined histologically were used for immunohistochemical (IHC) examination. Results of the present study did not show any significant differences in number of tachyzoites harvested from both cell line and also in mortality rate of inoculated embryonated eggs.Attenuation of virulence after 3 months continuous passages of suspension culture of TLI CELL LINE was not different with VERO CELL LINE whichthe results were confirmed by histopathology, IHC and PCR.Continuous passage ofNEOSPORA CANINUM in both cell lines suggesting that TLI cells could be used for vaccine production.

Cites

No record.

References

No record.

Cite

APA: Copy

KHORDADMEHR, M., ZEHTAB NAJAFI, M., NAMAVARI, M., & KHODA KARAM TAFTI, A.A.. (2017). COMPARISON OF PROPAGATION AND CONTINUOUS PASSAGES OF NEOSPORA CANINUM TACHYZOITES IN VERO AND TLI CELL LINES. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 13(3 ), 32-42. SID. https://sid.ir/paper/97046/en

Vancouver: Copy

KHORDADMEHR M., ZEHTAB NAJAFI M., NAMAVARI M., KHODA KARAM TAFTI A.A.. COMPARISON OF PROPAGATION AND CONTINUOUS PASSAGES OF NEOSPORA CANINUM TACHYZOITES IN VERO AND TLI CELL LINES. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2017;13(3 ):32-42. Available from: https://sid.ir/paper/97046/en

IEEE: Copy

M. KHORDADMEHR, M. ZEHTAB NAJAFI, M. NAMAVARI, and A.A. KHODA KARAM TAFTI, “COMPARISON OF PROPAGATION AND CONTINUOUS PASSAGES OF NEOSPORA CANINUM TACHYZOITES IN VERO AND TLI CELL LINES,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 13, no. 3 , pp. 32–42, 2017, [Online]. Available: https://sid.ir/paper/97046/en

Related Journal Papers