COMPARISON OF MICROSCOPICAL MEASURMENT AND PCR METHODS OF OOCYTE FOR DIFFERENTIATION OF EIMERIA TENELLA AND EIMERIA NECATRIX IN FECES OF BACK YARD HENS

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MAYAHI M.; JOLODAR A.; HAMIDINEJAT H.; SAIFY ABAD SHAPOURY M.; KHANDAN S.

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Paper Information

Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2010 | Volume:6 | Issue:3 (28) Page(s): 37-44

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Title

COMPARISON OF MICROSCOPICAL MEASURMENT AND PCR METHODS OF OOCYTE FOR DIFFERENTIATION OF EIMERIA TENELLA AND EIMERIA NECATRIX IN FECES OF BACK YARD HENS

Author(s)

Keywords

COCCIDIOSISQ3

EIMERIA TENELLAQ3

EIMERIA NECATRIXQ3

OOCYSTQ3

PCRQ1

LIGTH MICROSCOPEQ3

Abstract

The cause of COCCIDIOSIS is a protozoan parasite belongs to genus of Eimeria which multiply in the intestinal tract. The parasites damage the internal tissues and caused interruption of feeding and digestion processes, malfunction of nutrient absorption, dehydration, intestinal bleeding and increase susceptibility to other disease agents. Review of literatures indicates that no study has been done on the identification Eimeria nacatrix and EIMERIA TENELLA by OOCYST measurement and PCR methods. The aim of this study was the identification of EIMERIA TENELLA and EIMERIA NECATRIX in feces of the backyard hens by amplification of ITS-1 gene using PCR and also, identification of species by OOCYST measurement methods. Fifty feces samples from suspected backyard hen have been collected randomly. Two samples from each hen has been taken. One sample for routine OOCYST measurement and the other sample for ‍‍PCR method. Samples, were quickly transmitted to parasitology laboratory in the Faculty of Veterinary Medicine of Ahvaz and OOCYSTs separated by floating method. By using optical microscope, the shape and size of OOCYSTs were identified. The results of this study indicated that the contamination rate for E. tenella in PCR and measurement test was 48% and 34%, respectively and for E. necatrix was 6% in PCR and10% in OOCYST measurement test. Both species of Eimeria can produce acute COCCIDIOSIS in backyard hens and these can be regarded as a potential source of contamination in poultry farm industry. It was concluded that PCR is better method compare to routine oocyct measurement test for identification of Eimeria species. The rate of EIMERIA TENELLA infection was higher than EIMERIA NECATRIX. Therefore, the backyard hens may play important roles in epidemiology and spread contamination of E. necatrix and E. tenella in poultry farm industry. Rate of correlation between two methods for E. tenella and E.necatrix were 0.24 and 0.47, respectively.

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APA: Copy

MAYAHI, M., JOLODAR, A., HAMIDINEJAT, H., SAIFY ABAD SHAPOURY, M., & KHANDAN, S.. (2010). COMPARISON OF MICROSCOPICAL MEASURMENT AND PCR METHODS OF OOCYTE FOR DIFFERENTIATION OF EIMERIA TENELLA AND EIMERIA NECATRIX IN FECES OF BACK YARD HENS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 6(3 (28)), 37-44. SID. https://sid.ir/paper/97109/en

Vancouver: Copy

MAYAHI M., JOLODAR A., HAMIDINEJAT H., SAIFY ABAD SHAPOURY M., KHANDAN S.. COMPARISON OF MICROSCOPICAL MEASURMENT AND PCR METHODS OF OOCYTE FOR DIFFERENTIATION OF EIMERIA TENELLA AND EIMERIA NECATRIX IN FECES OF BACK YARD HENS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2010;6(3 (28)):37-44. Available from: https://sid.ir/paper/97109/en

IEEE: Copy

M. MAYAHI, A. JOLODAR, H. HAMIDINEJAT, M. SAIFY ABAD SHAPOURY, and S. KHANDAN, “COMPARISON OF MICROSCOPICAL MEASURMENT AND PCR METHODS OF OOCYTE FOR DIFFERENTIATION OF EIMERIA TENELLA AND EIMERIA NECATRIX IN FECES OF BACK YARD HENS,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 6, no. 3 (28), pp. 37–44, 2010, [Online]. Available: https://sid.ir/paper/97109/en

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