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Information Journal Paper

Title

SEX VERIFICATION OF ARAB HORSE USING PCR-RFLP WITHIN ZFX/ZFY GENES

Pages

  17-21

Abstract

 In order to investigatie the possibility and accuracy of horse SEX DETERMINATION using PCRRFLP within ZFX/ZFY Genes, whole blood samples of 20 ARAB HORSEs (l0 samples from female and 10 samples from male) were collected from Khozestan province. DNA was extracted using the modified salting-out procedure. Polymerase Chain Reaction (PCR), for amplification of 448 bp in coding region of ZFX/ZFY, was done using the primers, designed based on bovine ZFX/ZFY sequence. PCR products were digested by BSURI (HaeIII) restriction enzyme. PCR-RFLP genotypes consisted of 2 (403 and 45 bp) and 3 (448, 403 and 45 bp) bands for female and male, respectively. Results indicated that SEX DETERMINATION accuracy in animals was 100%. The successful PCR for equine using bovine primers indicated high conservation and homology in ZFX/ZFY sequence between two species horse and cattle. In conclusion, this gene is a reliable candidate gene for horse SEX DETERMINATION that can be used in SEX DETERMINATION of horse embryos sex.

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  • Cite

    APA: Copy

    JAVANMARD, A., ASADZADEH, N., BANA BAZI, M.H., & GHAFARI LALEH, V.. (2009). SEX VERIFICATION OF ARAB HORSE USING PCR-RFLP WITHIN ZFX/ZFY GENES. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 5(2 (23)), 17-21. SID. https://sid.ir/paper/97153/en

    Vancouver: Copy

    JAVANMARD A., ASADZADEH N., BANA BAZI M.H., GHAFARI LALEH V.. SEX VERIFICATION OF ARAB HORSE USING PCR-RFLP WITHIN ZFX/ZFY GENES. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2009;5(2 (23)):17-21. Available from: https://sid.ir/paper/97153/en

    IEEE: Copy

    A. JAVANMARD, N. ASADZADEH, M.H. BANA BAZI, and V. GHAFARI LALEH, “SEX VERIFICATION OF ARAB HORSE USING PCR-RFLP WITHIN ZFX/ZFY GENES,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 5, no. 2 (23), pp. 17–21, 2009, [Online]. Available: https://sid.ir/paper/97153/en

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