THE PHYLOGENETIC ANALYSIS OF SOME MYCOPLASMA SYNOVIAE ISOLATES FROM MAZANDRAN PROVINCE POULTRY FLOCKS

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HOSSEINI S.A.O.; POURBAKHSH S.A.; CHARKHKAR S.; BOZORG MEHRIFARD M.H.; SHEYKHI NARIMAN

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Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2010 | Volume:6 | Issue:1 (26) Page(s): 5-11

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Title

THE PHYLOGENETIC ANALYSIS OF SOME MYCOPLASMA SYNOVIAE ISOLATES FROM MAZANDRAN PROVINCE POULTRY FLOCKS

Author(s)

Keywords

MYCOPLASMA SYNOVIAEQ2

PHYLOGENETICQ2

MAZANDRANQ1

IRANQ2

Abstract

MYCOPLASMA SYNOVIAE (MS) is one of the important pathogens in chicken and turkey which causes great economic losses in poultry industry. The aim of this study was DNA sequence analysis of some MYCOPLASMA SYNOVIAE isolates from MAZANDRAN province poultry flocks and compares them with MS from other countries. For this purpose, 32 samples were collected from choanal cleft, trachea and air sac of flocks with respiratory symptom and cultured in specific PPLO medium. Ten out of 32 samples were positive in culture and reacted with specific antisera. The 10 positive samples were subsequently used for molecular study. The PCR products containing 16s rRNA genome were generated with specific primers and then sequenced. Multiple alignment and phylogenie analysis of DNA sequences were performed with DNASTAR program.Phylogenie analysis of MS isolated from MAZANDRAN province and 21 MS from the entire world revealed low homology among most of them. However some of the MAZANDRAN isolates showed high homology with Ms isolated from Brasilia. Overall, these results revealed the different molecular structure and heterogeneity among MS isolated from MAZANDRAN province in this study.

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APA: Copy

HOSSEINI, S.A.O., POURBAKHSH, S.A., CHARKHKAR, S., BOZORG MEHRIFARD, M.H., & SHEYKHI, NARIMAN. (2010). THE PHYLOGENETIC ANALYSIS OF SOME MYCOPLASMA SYNOVIAE ISOLATES FROM MAZANDRAN PROVINCE POULTRY FLOCKS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 6(1 (26)), 5-11. SID. https://sid.ir/paper/97167/en

Vancouver: Copy

HOSSEINI S.A.O., POURBAKHSH S.A., CHARKHKAR S., BOZORG MEHRIFARD M.H., SHEYKHI NARIMAN. THE PHYLOGENETIC ANALYSIS OF SOME MYCOPLASMA SYNOVIAE ISOLATES FROM MAZANDRAN PROVINCE POULTRY FLOCKS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2010;6(1 (26)):5-11. Available from: https://sid.ir/paper/97167/en

IEEE: Copy

S.A.O. HOSSEINI, S.A. POURBAKHSH, S. CHARKHKAR, M.H. BOZORG MEHRIFARD, and NARIMAN SHEYKHI, “THE PHYLOGENETIC ANALYSIS OF SOME MYCOPLASMA SYNOVIAE ISOLATES FROM MAZANDRAN PROVINCE POULTRY FLOCKS,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 6, no. 1 (26), pp. 5–11, 2010, [Online]. Available: https://sid.ir/paper/97167/en

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