EXPRESSION OF NSI GENE OF AVIAN INFLUENZA H9N2 VIRUS

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TALAZADEH F.; SEYFI ABAD SHAPOURI M.R.; MAYAHI M.; ESLAMI M.; JAYDARI A.; DAGHARI M.

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Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2012 | Volume:7 | Issue:4 (33) Page(s): 38-45

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Title

EXPRESSION OF NSI GENE OF AVIAN INFLUENZA H9N2 VIRUS

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Keywords

AVIAN INFLUENZAQ2

H9N2 SUBTYPEQ2

NSI GENEQ2

Abstract

AVIAN INFLUENZA Virus (AIV) is classified in the genus of influenza virus A within the Orthomyxoviridae family. The aim of this study was the cloning and expression of NSI GENE belongs to an AVIAN INFLUENZA virus isolate (AiChicken/Iran/AH-l/06), serotype H9N2 from a broiler chicken flock in Ahvaz, with respiratory signs and mortality. The NS 1 protein is used for the DIVA test (Differentiating Infected from Vaccinated Animals) in the form of an ELISA test. For this purpose several nucleotide sequence of the NS 1 coding region (from nucleotide 27 to 680) of AVIAN INFLUENZA virus subtype H9N2 retrieved extracted from the Gene bank in order to design the required primers. The NS 1 gene was subsequently amplified by RT - PCR. The vector, pMAL-c2X and the amplified NSI GENE, both were digested by EcoRI and Pstl restriction endonucleases and ligated by T4 DNA ligase. The construct was then transformed into BL strain of E. coli and finally the purified recombinant vector was used for expression of NS 1 protein. The 66.4 KDa protein was identified by both SDS-PAGE and Western blotting. Observation of expressed protein with the expected molecular weight in SDS-PAGE and a positive reaction with AVIAN INFLUENZA virus H9N2 infected chicks sera by Western blotting revealed a successful expression of the segment ofNSl gene in Eicoli.

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APA: Copy

TALAZADEH, F., SEYFI ABAD SHAPOURI, M.R., MAYAHI, M., ESLAMI, M., JAYDARI, A., & DAGHARI, M.. (2012). EXPRESSION OF NSI GENE OF AVIAN INFLUENZA H9N2 VIRUS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 7(4 (33)), 38-45. SID. https://sid.ir/paper/97180/en

Vancouver: Copy

TALAZADEH F., SEYFI ABAD SHAPOURI M.R., MAYAHI M., ESLAMI M., JAYDARI A., DAGHARI M.. EXPRESSION OF NSI GENE OF AVIAN INFLUENZA H9N2 VIRUS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2012;7(4 (33)):38-45. Available from: https://sid.ir/paper/97180/en

IEEE: Copy

F. TALAZADEH, M.R. SEYFI ABAD SHAPOURI, M. MAYAHI, M. ESLAMI, A. JAYDARI, and M. DAGHARI, “EXPRESSION OF NSI GENE OF AVIAN INFLUENZA H9N2 VIRUS,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 7, no. 4 (33), pp. 38–45, 2012, [Online]. Available: https://sid.ir/paper/97180/en

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