THE STUDY OF PROBABLE VARIATION IN ITS- RDNA REGION OF FUSARIUM SOLANI IN POTATO AND ITS CORRELATION WITH PATHOGENICITY AND GEOGRAPHICAL ORIGIN IN RAZAVI AND NORTHERN KHORASAN PROVINCES

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BAGHAEI RAVARI S.; FALAHATI RASTGAR M.; JAFARPOUR B.; SHOKOUHIFAR F.

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Journal: Journal of Agricultural Sciences and Natural Resources Year:2007 | Volume:14 | Issue:3 Page(s): 120-128

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Title

THE STUDY OF PROBABLE VARIATION IN ITS- RDNA REGION OF FUSARIUM SOLANI IN POTATO AND ITS CORRELATION WITH PATHOGENICITY AND GEOGRAPHICAL ORIGIN IN RAZAVI AND NORTHERN KHORASAN PROVINCES

Author(s)

BAGHAEI RAVARI S. | FALAHATI RASTGAR M. | JAFARPOUR B. | SHOKOUHIFAR F. | Issue Writer Certificate

Keywords

FUSARIUM SOLANIQ3

PCR- RIBOTYPINGQ2

POTATOQ3

Abstract

Lack of adequate information regarding genotypic diversity within populations of plant pathogenic fungi is one of the most critical factors limiting breeding programs. During the growing seasons of 2003-2004, twenty two FUSARIUM SOLANI isolates, the casual agent of wilting and dry rot of POTATO were obtained from fields and storages of Razavi and Northern Khorasan provinces. The pathogen city tests were carried out by soil-infestation, root-dipping, injection of spore suspension into tubers and tuber inoculation with a piece of media covered with mycelia on susceptible cultivar, Agria. In order to study the variation of the rDAN gene cluster, PCR-ribotyping was done on 22 isolates of Razavi and Northern Khorasan provinces and six isolates of Tehran, Hamadan and Ardabil provinces. Also the amplification of ITS-rDNA was subjected to digest by EcoRI and ReaIII enzymes. The results revealed that dry rot occurred by all isolates, soft rot by 28% and wilting symptoms by 76% of the total 22 isolates used in this experiment. The amplification of rDNA-ITS divided all isolates into ITS1 and ITS2 types. ReaIII showed the polymorphism in number and length of digested fragments better than EcoRI. In this study, there was no obvious evidence of any correlation between geographical origion, pathogenicity and ITS types of isolates.

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APA: Copy

BAGHAEI RAVARI, S., FALAHATI RASTGAR, M., JAFARPOUR, B., & SHOKOUHIFAR, F.. (2007). THE STUDY OF PROBABLE VARIATION IN ITS- RDNA REGION OF FUSARIUM SOLANI IN POTATO AND ITS CORRELATION WITH PATHOGENICITY AND GEOGRAPHICAL ORIGIN IN RAZAVI AND NORTHERN KHORASAN PROVINCES. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 14(3), 120-128. SID. https://sid.ir/paper/9720/en

Vancouver: Copy

BAGHAEI RAVARI S., FALAHATI RASTGAR M., JAFARPOUR B., SHOKOUHIFAR F.. THE STUDY OF PROBABLE VARIATION IN ITS- RDNA REGION OF FUSARIUM SOLANI IN POTATO AND ITS CORRELATION WITH PATHOGENICITY AND GEOGRAPHICAL ORIGIN IN RAZAVI AND NORTHERN KHORASAN PROVINCES. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2007;14(3):120-128. Available from: https://sid.ir/paper/9720/en

IEEE: Copy

S. BAGHAEI RAVARI, M. FALAHATI RASTGAR, B. JAFARPOUR, and F. SHOKOUHIFAR, “THE STUDY OF PROBABLE VARIATION IN ITS- RDNA REGION OF FUSARIUM SOLANI IN POTATO AND ITS CORRELATION WITH PATHOGENICITY AND GEOGRAPHICAL ORIGIN IN RAZAVI AND NORTHERN KHORASAN PROVINCES,” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 14, no. 3, pp. 120–128, 2007, [Online]. Available: https://sid.ir/paper/9720/en

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