THE STUDY OF SARABI CATTLE KARYOTYPE INVESTIGATION BY GTG – BANDING

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PIRA HARI O.; MEMARIAN M.; SHOJAA J.; HARAKI NEJAD T.

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ISSN: 2588-4824

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Paper Information

Journal: Journal of Agricultural Sciences and Natural Resources Year:2007 | Volume:14 | Issue:3 Page(s): 98-110

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Title

THE STUDY OF SARABI CATTLE KARYOTYPE INVESTIGATION BY GTG – BANDING

Author(s)

PIRA HARI O. | MEMARIAN M. | SHOJAA J. | HARAKI NEJAD T. | Issue Writer Certificate

Keywords

CYTOGENETICQ3

CHROMOSOMEQ3

KARYOTYPEQ3

GTG-BANDINGQ3

SARABI CATTLEQ3

Abstract

A CYTOGENETIC study was conducted on 30 SARABI CATTLEs in SARABI CATTLE Research Center in Shabestar. Under completely sterile conditions, whole blood samples were collected from jugular vein using sterile syringes. About 5-6 drops of whole blood were cultured in 5 ml of RPMI1640 which was supplemented with 20% F.C.S. 1% antibiotics (penicillin, streptomycin), 2% phytohemagglutinin M (PHA-M), and 1% heparin. F.C.S. and phytohemagglutinin M were used as growth parameter factor and mitotic activity stimulator, respectively. Samples were cultured in the incubator to accomplish for 69-71 hours at 37°C. For normal staining, 10% Giemsa solution was used. But for GTG – banding staining, the slides were first treated by 0.25% trypsin and then with 4% Giemsa solution, and finally the slides were dried. The analysis revealed that SARABI CATTLE like many species belongine to genus BQS had 60 CHROMOSOMEs which consisted of 29 pairs of autosomes and one pair of sex chromosems. All autosomes were acrocentric and all the sex CHROMOSOMEs were submetacentric. X CHROMOSOMEs were greater than Y CHROMOSOMEs.

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APA: Copy

PIRA HARI, O., MEMARIAN, M., SHOJAA, J., & HARAKI NEJAD, T.. (2007). THE STUDY OF SARABI CATTLE KARYOTYPE INVESTIGATION BY GTG – BANDING. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 14(3), 98-110. SID. https://sid.ir/paper/9724/en

Vancouver: Copy

PIRA HARI O., MEMARIAN M., SHOJAA J., HARAKI NEJAD T.. THE STUDY OF SARABI CATTLE KARYOTYPE INVESTIGATION BY GTG – BANDING. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2007;14(3):98-110. Available from: https://sid.ir/paper/9724/en

IEEE: Copy

O. PIRA HARI, M. MEMARIAN, J. SHOJAA, and T. HARAKI NEJAD, “THE STUDY OF SARABI CATTLE KARYOTYPE INVESTIGATION BY GTG – BANDING,” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 14, no. 3, pp. 98–110, 2007, [Online]. Available: https://sid.ir/paper/9724/en

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