ISOLATION AND DETERMINATION OF THE VIRULENCE GENES OF ESCHERICHIA COLI 0157 IN GROUND BEEFS AND HAMBURGERS IN SHAHREKORD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BONYADIAN M.; ZAHRAEI SALEHI TAGHI; MOMTAZ H.; HASANPOUR A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2010 | Volume:5 | Issue:4 (25) Page(s): 5-12

Download Full-Text

Persian Verion

View:

1,194

Download:

Cites:

Information Journal Paper

Title

ISOLATION AND DETERMINATION OF THE VIRULENCE GENES OF ESCHERICHIA COLI 0157 IN GROUND BEEFS AND HAMBURGERS IN SHAHREKORD

Author(s)

BONYADIAN M. | ZAHRAEI SALEHI TAGHI | MOMTAZ H. | HASANPOUR A. | Issue Writer Certificate

Keywords

ESCHERICHIA COLI 0157Q3

GROUND BEEFQ3

HAMBURGERQ1

VIRULENCE GENESQ2

Abstract

The aim of this study was to determine the quality of ground meats and HAMBURGERs and the contamination of these products with E. coli 0157. A total of 250 samples (125 ground meats and 125 HAMBURGERs) were collected for retails in Shahrekord. Microbiological examinations were done on the samples for coliform counting by making tenfold serial dilution and plating them with Mc.A media. To isolate the E. coli 0157, samples were cultured in E.E broth containing Novobiocin and incubated at 37°C overnight. Subcultures were prepared on MC Sorbitol agar and incubated at 37°C for 24h. Suspected colonies were chosen, biochemical and serological tests were done on the colonies to confmn the E. coli 0157 and 0157:H7. The results of this study showed that the microbial quality of GROUND BEEFs and HAMBURGERs are unacceptable according to the standard limit of Iran, the mean value of coliform were 1.75x103 CFU/g and 5.7 x102 CFU/g respectively. Indeed 4 (3.2%) of the GROUND BEEFs and HAMBURGERs were contaminated with E. coli 0157, but none of them were E. coli 0157:H7. PCR for detecting the VIRULENCE GENES among the isolated E.coli 0157 revealed that 37.7 % contain VT2 and eae genes. But other VIRULENCE GENES (hly and VTl) were shown in none of the isolates.

Cites

No record.

References

No record.

Cite

APA: Copy

BONYADIAN, M., ZAHRAEI SALEHI, TAGHI, MOMTAZ, H., & HASANPOUR, A.. (2010). ISOLATION AND DETERMINATION OF THE VIRULENCE GENES OF ESCHERICHIA COLI 0157 IN GROUND BEEFS AND HAMBURGERS IN SHAHREKORD. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 5(4 (25)), 5-12. SID. https://sid.ir/paper/97287/en

Vancouver: Copy

BONYADIAN M., ZAHRAEI SALEHI TAGHI, MOMTAZ H., HASANPOUR A.. ISOLATION AND DETERMINATION OF THE VIRULENCE GENES OF ESCHERICHIA COLI 0157 IN GROUND BEEFS AND HAMBURGERS IN SHAHREKORD. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2010;5(4 (25)):5-12. Available from: https://sid.ir/paper/97287/en

IEEE: Copy

M. BONYADIAN, TAGHI ZAHRAEI SALEHI, H. MOMTAZ, and A. HASANPOUR, “ISOLATION AND DETERMINATION OF THE VIRULENCE GENES OF ESCHERICHIA COLI 0157 IN GROUND BEEFS AND HAMBURGERS IN SHAHREKORD,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 5, no. 4 (25), pp. 5–12, 2010, [Online]. Available: https://sid.ir/paper/97287/en

Related Journal Papers