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Information Journal Paper

Title

THE INVESTIGATION OF THE EFFECT OF VARIOUS HORMONAL COMPOUNDS AND COCONUT MILK ON THE GROWTH AND PRODUCTION OF CHINESE ELM CALLUS (ULMUS PARVIFOLIA JACQ.)

Pages

  155-163

Abstract

 One of the main methods for the mass production of CALLUS is the use of the technique of the plant TISSUE CULTURE. The purpose of this study was the production of CHINESE ELM CALLUS Ulmus parvifolia Jacq. by application of COCONUT MILK. Samples of tip shoots and shoots with buds, after surface disinfection with ethanol and sodium hypochlorite were cultured on the Murashige & Skoog (MS) and Gamburg (Bs) media culture. The effect of different compounds and COCONUT MILK was compared in these two media on growth of calli. The trial was established in the randomized complete block design with 14 treatments and three replications in vitro. Treatments were two basic media culture of MS and B5 which contained COCONUT MILK with 2,4-dicholoro phenoxy acetic acid (2,4-D), Benzylamino purine (BAP), Kinetin (Kin), Naphthalene acetic acid (NAA) and control were without MS and B5 media cultures plant growth regulator. The results showed that coconut application within MS media culture increased significantly weight of calli (2.37g) 35 days after incubation while there was not significant (p=%5) differences between various treatments in B5 media culture. Among of calli, CALLUS were grown on MS containing COCONUT MILK had significantly greater weight (%31.7) than treatments without COCONUT MILK. Morphological characters of CALLUS in agar media culture with COCONUT MILK were produced yellow cell tissue, hard and intensive tissues. In the treatments containing only 2, 4-D CALLUS weight decreased significantly. This study is the first report that investigated on this species in Iran and Asia.

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    APA: Copy

    MAMGHANI GHAZI JAHANI, M., MASHAYEKHI, K., & RAHNAMA, K.. (2007). THE INVESTIGATION OF THE EFFECT OF VARIOUS HORMONAL COMPOUNDS AND COCONUT MILK ON THE GROWTH AND PRODUCTION OF CHINESE ELM CALLUS (ULMUS PARVIFOLIA JACQ.). JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 14(3), 155-163. SID. https://sid.ir/paper/9730/en

    Vancouver: Copy

    MAMGHANI GHAZI JAHANI M., MASHAYEKHI K., RAHNAMA K.. THE INVESTIGATION OF THE EFFECT OF VARIOUS HORMONAL COMPOUNDS AND COCONUT MILK ON THE GROWTH AND PRODUCTION OF CHINESE ELM CALLUS (ULMUS PARVIFOLIA JACQ.). JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2007;14(3):155-163. Available from: https://sid.ir/paper/9730/en

    IEEE: Copy

    M. MAMGHANI GHAZI JAHANI, K. MASHAYEKHI, and K. RAHNAMA, “THE INVESTIGATION OF THE EFFECT OF VARIOUS HORMONAL COMPOUNDS AND COCONUT MILK ON THE GROWTH AND PRODUCTION OF CHINESE ELM CALLUS (ULMUS PARVIFOLIA JACQ.),” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 14, no. 3, pp. 155–163, 2007, [Online]. Available: https://sid.ir/paper/9730/en

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