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Information Journal Paper

Title

CLONING AND EXPRESSION OF CHLAMYDOPHILA ABORTUS SEGMENT OF POMP90 GENE CODING IN E. COLI

Pages

  74-80

Abstract

CHLAMYDOPHILA ABORTUS is one of the major causes of infectious ABORTION in pregnant sheep worldwide. Organisms shed in infected placentas and uterine discharges at lambing time are the main sources of environmental contamination, responsible for transmission to susceptible animals and possible human contacts. In this study one segment with 375 bp length of POMP90 GENE coding region of CHLAMYDOPHILA ABORTUS S26/3 (Tyr113-Asn238) was amplified with PCR and expressed in E.coli BL-21, after cloning in pMAL-C2X vector. The recombinant 55 kDa protein was identified by both SDS-PAGE and immunoblotting. Expression of a protein with the expected molecular weight in SDS-PAGE and a positive reaction of the protein with CHLAMYDOPHILA ABORTUS infected sheep sera by immunoblotting, revealed a successful expression of POMP90 GENE segment in E. coli.

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    APA: Copy

    MOORI BAKHTIARI, N., SEYFIABAD SHAPOURI, M.R., GHORBANPOOR, M., & GOORANINEJAD, S.. (2011). CLONING AND EXPRESSION OF CHLAMYDOPHILA ABORTUS SEGMENT OF POMP90 GENE CODING IN E. COLI. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 7(3 (32)), 74-80. SID. https://sid.ir/paper/97424/en

    Vancouver: Copy

    MOORI BAKHTIARI N., SEYFIABAD SHAPOURI M.R., GHORBANPOOR M., GOORANINEJAD S.. CLONING AND EXPRESSION OF CHLAMYDOPHILA ABORTUS SEGMENT OF POMP90 GENE CODING IN E. COLI. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2011;7(3 (32)):74-80. Available from: https://sid.ir/paper/97424/en

    IEEE: Copy

    N. MOORI BAKHTIARI, M.R. SEYFIABAD SHAPOURI, M. GHORBANPOOR, and S. GOORANINEJAD, “CLONING AND EXPRESSION OF CHLAMYDOPHILA ABORTUS SEGMENT OF POMP90 GENE CODING IN E. COLI,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 7, no. 3 (32), pp. 74–80, 2011, [Online]. Available: https://sid.ir/paper/97424/en

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