COMPARISON OF MICROBIAL CULTURE, PCR AND CMT FOR DIAGNOSIS OF DAIRY CATTLE'S STAPHYLOCOCCAL AND STREPTOCOCCAL SUBCLINICAL MASTITIS

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GHORBANPOUR MASOUD; SEYFIABAD SHAPOURI M.; MOATAMEDI H.; JAMSHIDIAN MAHMOUD; GOURANINEZHAD S.

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Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2007 | Volume:3 | Issue:1 (14) Page(s): 63-70

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Title

COMPARISON OF MICROBIAL CULTURE, PCR AND CMT FOR DIAGNOSIS OF DAIRY CATTLE'S STAPHYLOCOCCAL AND STREPTOCOCCAL SUBCLINICAL MASTITIS

Author(s)

Keywords

MICROBIAL CULTUREQ2

PCRQ2

CMTQ3

SUBCLINICAL MASTITISQ2

CATTLEQ3

Abstract

To evaluate CMT for diagnosis of dairy CATTLE's SUBCLINICAL MASTITIS caused by S. aureus, S. agalactiae, S. dysgalactiae, and S. uberis , 100 CMT positive and 20 CMT negative milk samples were collected and examined by PCR and conventional bacterial culture methods for obove agents and the results of each methods were compared with CMT results. In bacterial culture, S. mtreus was isolated from 25 samples; all had positive PCR and CMT reactions.The sensitivity of CMT for diagnosis of S. aureus SUBCLINICAL MASTITIS was 100%. In bacterial culture, S. agalactiae was isolated from 19 samples, 15 with positive CMT reaction, and 4 from CMT negative samples. S. dysgalactiae was isolated from 10 samples, seven of which were CMT positive and three were CMT negative. S. uberis was only isolated from one CMT positive sample. There was not any streptococcal culture of positive sample being negative in PCR, but there were 10 PCR positives being negative in culture method. There were 14 and 7 CMT negative samples being positive by PCR and culture methods respectively, so the sensitivity of CMT for detection of streptococcal SUBCLINICAL MASTITIS comparing to PCR and culture methods was 65% and 75% respectively.In conclusion, CMT has not sufficient sensitivity for detection of SUBCLINICAL MASTITIS due to these streptococcal agents but has 100% sensitivity for detection of SUBCLINICAL MASTITIS due to S.aureus.

Cites

بررسی الگوی مقاومت آنتی بیوتیکی و ردیابی سویه های مقاوم به متی سیلین (MRSA) در ایزوله های استافیلوکوکوس اورئوس عامل ورم پستان گاوی در استان اصفهان

References

SENSITIVITY AND SPECIFICITY OF CMT TO DETECT SUB CLINICAL MASTITIS IN DAIRY COWS

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APA: Copy

GHORBANPOUR, MASOUD, SEYFIABAD SHAPOURI, M., MOATAMEDI, H., JAMSHIDIAN, MAHMOUD, & GOURANINEZHAD, S.. (2007). COMPARISON OF MICROBIAL CULTURE, PCR AND CMT FOR DIAGNOSIS OF DAIRY CATTLE'S STAPHYLOCOCCAL AND STREPTOCOCCAL SUBCLINICAL MASTITIS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 3(1 (14)), 63-70. SID. https://sid.ir/paper/97481/en

Vancouver: Copy

GHORBANPOUR MASOUD, SEYFIABAD SHAPOURI M., MOATAMEDI H., JAMSHIDIAN MAHMOUD, GOURANINEZHAD S.. COMPARISON OF MICROBIAL CULTURE, PCR AND CMT FOR DIAGNOSIS OF DAIRY CATTLE'S STAPHYLOCOCCAL AND STREPTOCOCCAL SUBCLINICAL MASTITIS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2007;3(1 (14)):63-70. Available from: https://sid.ir/paper/97481/en

IEEE: Copy

MASOUD GHORBANPOUR, M. SEYFIABAD SHAPOURI, H. MOATAMEDI, MAHMOUD JAMSHIDIAN, and S. GOURANINEZHAD, “COMPARISON OF MICROBIAL CULTURE, PCR AND CMT FOR DIAGNOSIS OF DAIRY CATTLE'S STAPHYLOCOCCAL AND STREPTOCOCCAL SUBCLINICAL MASTITIS,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 3, no. 1 (14), pp. 63–70, 2007, [Online]. Available: https://sid.ir/paper/97481/en

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