Antitumor Activities of Green Tea by Up-regulation of miR-181a Expression in LNCaP Cells Using 3D Cell Culture Model

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SAFARI FATEMEH; Rayat Azad Narjes; Alizadeh Ezdiny Ali; Pakizehkar Safura; KHAZAEI KOOHPAR ZEINAB; RANJI NAJMEH

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Journal: Avicenna Journal of Medical Biotechnology Year:2022 | Volume:14 | Issue:1 Page(s): 89-94

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Title

Antitumor Activities of Green Tea by Up-regulation of miR-181a Expression in LNCaP Cells Using 3D Cell Culture Model

Author(s)

Keywords

Apoptosis

Cell culture techniques

Green tea

Proto-oncogene proteins c-bcl-2

Upregulation

Abstract

Background: Prostate Cancer (PCa) is the major reason for the high mortality rates among men worldwide. In fact, current therapeutic approaches are not successful. It appears that discovering more effective methods considering several parameters such as availability, low cost, and no toxicity to normal cells is one of the biggest challenges for interested researchers. Green tea (extracted from the plant Camellia sinensis) with high level of polyphenolic compounds and as the most globally consumed beverage has attracted considerable interest. MicroRNAs (or miRNAs) were considered as novel tools in cancer therapy which modulate various biological events in cell by regulation of gene expression. The aim of the current study was to evaluate the antitumor activity of Green tea in LNCaP cells through up-regulation of miR-181a expression. Methods: First, LNCaP cells were cultured and by using quantitative real time PCR (qRT-PCR) and western blot methods, the expression levels of Bax and BCL2 were analyzed. Next, a 3D cell culture model was applied to evaluate the expression of miRNA-181a in LNCaP cells. Results: It was shown that Green tea induced cellular Apoptosis. The high number of apoptotic nuclei was also shown by using DAPI staining. The inhibition of tumor growth was revealed by analyzing the size and number of spheroids. Also, Upregulation of miR-181a expression in LNCaP cells was revealed after treatment with Green tea. Conclusion: Our results are helpful to design antitumor regimens based on consumption of Green tea through up-regulation of miRNA-181a expression and induction of Apoptosis.

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APA: Copy

SAFARI, FATEMEH, Rayat Azad, Narjes, Alizadeh Ezdiny, Ali, Pakizehkar, Safura, KHAZAEI KOOHPAR, ZEINAB, & RANJI, NAJMEH. (2022). Antitumor Activities of Green Tea by Up-regulation of miR-181a Expression in LNCaP Cells Using 3D Cell Culture Model. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 14(1), 89-94. SID. https://sid.ir/paper/977007/en

Vancouver: Copy

SAFARI FATEMEH, Rayat Azad Narjes, Alizadeh Ezdiny Ali, Pakizehkar Safura, KHAZAEI KOOHPAR ZEINAB, RANJI NAJMEH. Antitumor Activities of Green Tea by Up-regulation of miR-181a Expression in LNCaP Cells Using 3D Cell Culture Model. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2022;14(1):89-94. Available from: https://sid.ir/paper/977007/en

IEEE: Copy

FATEMEH SAFARI, Narjes Rayat Azad, Ali Alizadeh Ezdiny, Safura Pakizehkar, ZEINAB KHAZAEI KOOHPAR, and NAJMEH RANJI, “Antitumor Activities of Green Tea by Up-regulation of miR-181a Expression in LNCaP Cells Using 3D Cell Culture Model,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 14, no. 1, pp. 89–94, 2022, [Online]. Available: https://sid.ir/paper/977007/en

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