Fluorescent Detection of Methicillin Resistant Staphylococcus aureus by Loop-mediated Isothermal Amplification Assisted with Streptavidin-coated Quantum Dots

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ALIASGHARIAN AILY; GILL POORIA; AHANJAN MOHAMMAD; RAFATI ADELE

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Journal Paper

Paper Information

Journal: Avicenna Journal of Medical Biotechnology Year:2022 | Volume:14 | Issue:1 Page(s): 79-88

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Title

Fluorescent Detection of Methicillin Resistant Staphylococcus aureus by Loop-mediated Isothermal Amplification Assisted with Streptavidin-coated Quantum Dots

Author(s)

ALIASGHARIAN AILY | GILL POORIA | AHANJAN MOHAMMAD | RAFATI ADELE | Issue Writer Certificate

Keywords

LAMP

MRSA

MSSA

Quantum dots

mecA gene

Abstract

Background: Methicillin Resistance Staphylococcus aureus (MRSA) could be considered as a major concern in medicine can cause nosocomial infection and bacteremia, especially in patients using catheter and household medical devices. Methods: Using molecular diagnostic methods are important for identification of MRSA from the Methicillin Sensitive Staphylococcus aureus (MSSA). Here we described a fluorescent assay using biotin-labelling Loop-mediated isothermal amplification (LAMP) method assisted with streptavidincoated Quantum dots (QDs) for detection of MRSA. For comparison, another fluorescent assay using LAMP assisted with Green Viewer (GV; a fluorescent dye) was applied for detection of MRSA. The mecA gene was selected as the target for amplification by LAMP and for biotin-labeling of the LAMP amplicons, biotin-11-dUTP was mixed with the dNTPs (deoxy Nucleotide Phosphates) in LAMP reaction. For determining the clinical performance of the developed assay, 30 blood samples with MRSA positive results were tested with QD-LAMP, the conventional LAMP, GVLAMP, and Polymerase Chain Reaction (PCR). Results: Obtained results indicated that % sensitivity of QD-LAMP was 86. 66% for detection of mecA positive MRSA samples; however, the Limit of Detection (LoD) of QD-LAMP was 1. 5×104 Colony Forming Unit (CFU). Conclusion: The results suggested that the QD-LAMP assay was easy to operate and could be used for detection of MRSA in parallel to the blood culture with less sensitivity for detection of bacteremia and pediatric septicemia with low counts of MRSA.

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APA: Copy

ALIASGHARIAN, AILY, GILL, POORIA, AHANJAN, MOHAMMAD, & RAFATI, ADELE. (2022). Fluorescent Detection of Methicillin Resistant Staphylococcus aureus by Loop-mediated Isothermal Amplification Assisted with Streptavidin-coated Quantum Dots. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 14(1), 79-88. SID. https://sid.ir/paper/981738/en

Vancouver: Copy

ALIASGHARIAN AILY, GILL POORIA, AHANJAN MOHAMMAD, RAFATI ADELE. Fluorescent Detection of Methicillin Resistant Staphylococcus aureus by Loop-mediated Isothermal Amplification Assisted with Streptavidin-coated Quantum Dots. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2022;14(1):79-88. Available from: https://sid.ir/paper/981738/en

IEEE: Copy

AILY ALIASGHARIAN, POORIA GILL, MOHAMMAD AHANJAN, and ADELE RAFATI, “Fluorescent Detection of Methicillin Resistant Staphylococcus aureus by Loop-mediated Isothermal Amplification Assisted with Streptavidin-coated Quantum Dots,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 14, no. 1, pp. 79–88, 2022, [Online]. Available: https://sid.ir/paper/981738/en

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