INTRODUCTION OF LIVER INFUSION TRYPTOSE (LIT) FOR CULTIVATION AND DIAGNOSIS OF BLASTOSYSTIS SP.

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MOOSAVI ALI; HAGHIGHI ALI; ROSTAMI NEJAD MOHAMMAD; NAZEMALHOSSEINI MOJARAD EHSAN; NADERI FARIDEH; MOHAMMADIHA ANITA; RAFIEI SEFIDDASHTI RAHELE

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Journal: Medical Sciences Journal of Islamic Azad University,Tehran Medical Branch Year:2014 | Volume:23 | Issue:1 (SUPP.) Page(s): 39-42

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Title

INTRODUCTION OF LIVER INFUSION TRYPTOSE (LIT) FOR CULTIVATION AND DIAGNOSIS OF BLASTOSYSTIS SP.

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Keywords

BLASTOCYSTISQ2

CULTURE MEDIAQ3

DIAGNOSISQ3

Abstract

Background: According to some researches in recent years, BLASTOCYSTIS is considered as the etiological agent of some gastrointestinal disorders. This parasite is polymorphic and its detection is not easy under the microscope. Because of this problem, several microscopic based studies have already reported different BLASTOCYSTIS infection rates. Application of culture methods increases the chance of DIAGNOSIS of this parasite. The in vitro culture of BLASTOCYSTIS in Liver Infusion Tryptose (LIT) medium is described for the first time in this study.Materials and Methods: Liver Infusion Tryptose (LIT) containing 10% fetal calf serum in screw-capped tubes was used. Samples incubated in 37°c and examined after 24, 48 and 72 hours.Results: 420 fresh stool samples were tested by LIT culture method and 100 positive samples (23.8%) of BLASTOCYSTIS sp. were detected. while only 64 samples (15.2%) were positive with direct smear examination.Conclusion: This study highlights the same sensitivity of this CULTURE MEDIA and other regular cultures media and it introduces a new medium for BLASTOCYSTIS sp. detection.

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APA: Copy

MOOSAVI, ALI, HAGHIGHI, ALI, ROSTAMI NEJAD, MOHAMMAD, NAZEMALHOSSEINI MOJARAD, EHSAN, NADERI, FARIDEH, MOHAMMADIHA, ANITA, & RAFIEI SEFIDDASHTI, RAHELE. (2014). INTRODUCTION OF LIVER INFUSION TRYPTOSE (LIT) FOR CULTIVATION AND DIAGNOSIS OF BLASTOSYSTIS SP.. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 23(1 (SUPP.)), 39-42. SID. https://sid.ir/paper/98489/en

Vancouver: Copy

MOOSAVI ALI, HAGHIGHI ALI, ROSTAMI NEJAD MOHAMMAD, NAZEMALHOSSEINI MOJARAD EHSAN, NADERI FARIDEH, MOHAMMADIHA ANITA, RAFIEI SEFIDDASHTI RAHELE. INTRODUCTION OF LIVER INFUSION TRYPTOSE (LIT) FOR CULTIVATION AND DIAGNOSIS OF BLASTOSYSTIS SP.. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2014;23(1 (SUPP.)):39-42. Available from: https://sid.ir/paper/98489/en

IEEE: Copy

ALI MOOSAVI, ALI HAGHIGHI, MOHAMMAD ROSTAMI NEJAD, EHSAN NAZEMALHOSSEINI MOJARAD, FARIDEH NADERI, ANITA MOHAMMADIHA, and RAHELE RAFIEI SEFIDDASHTI, “INTRODUCTION OF LIVER INFUSION TRYPTOSE (LIT) FOR CULTIVATION AND DIAGNOSIS OF BLASTOSYSTIS SP.,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 23, no. 1 (SUPP.), pp. 39–42, 2014, [Online]. Available: https://sid.ir/paper/98489/en

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