EVALUATING THE EFFECT OF DMSO CONCENTRATIONS ON Y-GLOBIN EXPRESSION IN HULL CELLS

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ASGHARIAN A.M.; BANAN MAHDI; DEYLAMI Z.; GHAREH SOURAN J.; GHASEMI SAGHAR; BEHJATI F.; JAVADI GH.R.; KAHRIZI K.; NAJMABADI H.

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Journal: MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY Year:2008 | Volume:18 | Issue:2 (52) Page(s): 75-80

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Title

EVALUATING THE EFFECT OF DMSO CONCENTRATIONS ON Y-GLOBIN EXPRESSION IN HULL CELLS

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Keywords

HULL CELLSQ3

DMSOQ3

HEMOGLOBINQ3

γ-GLOBIN GENEQ3

Abstract

Background: Understanding of the mechanisms involved in g- to b-globin switching may be important for development of treatment options for b-thalassemia. Such studies require the availability of relevant cellular model systems. One such cell type is Hull, a mouse erythroleukemia (MEL) cell line containing the human b-Iocus. MEL and HULL CELLS differentiate in the presence of the chemical dimethyl sulfoxide (DMSO). Nevertheless, levels of g-globin induction in HULL CELLS after DMSO treatment have not been determined. In the present study, we determined g-globin levels in HULL CELLS after treatment with various DMSO concentrations.Materials and methods: HULL CELLS were cultivated in various DMSO concentrations and the levels of g-globin were determined by real-time PCR.Results: Our study showed that HEMOGLOBIN in HULL CELLS treated with I% and 2% DMSO was increased by approximately 5 and IO folds. Moreover real-time PCR results showed that g-globin levels using the indicated DMSO levels were increased by 66 and 298 folds, respectively.Conclusion: HULL CELLS differentiate in the presence of DMSO, and in doing so, their y-globin levels are increased. Therefore these cells can be used to study the mechanisms of g-globin induction. Such studies may be beneficial for the treatment of b-thalassemia.

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APA: Copy

ASGHARIAN, A.M., BANAN, MAHDI, DEYLAMI, Z., GHAREH SOURAN, J., GHASEMI, SAGHAR, BEHJATI, F., JAVADI, GH.R., KAHRIZI, K., & NAJMABADI, H.. (2008). EVALUATING THE EFFECT OF DMSO CONCENTRATIONS ON Y-GLOBIN EXPRESSION IN HULL CELLS. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 18(2 (52)), 75-80. SID. https://sid.ir/paper/98531/en

Vancouver: Copy

ASGHARIAN A.M., BANAN MAHDI, DEYLAMI Z., GHAREH SOURAN J., GHASEMI SAGHAR, BEHJATI F., JAVADI GH.R., KAHRIZI K., NAJMABADI H.. EVALUATING THE EFFECT OF DMSO CONCENTRATIONS ON Y-GLOBIN EXPRESSION IN HULL CELLS. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2008;18(2 (52)):75-80. Available from: https://sid.ir/paper/98531/en

IEEE: Copy

A.M. ASGHARIAN, MAHDI BANAN, Z. DEYLAMI, J. GHAREH SOURAN, SAGHAR GHASEMI, F. BEHJATI, GH.R. JAVADI, K. KAHRIZI, and H. NAJMABADI, “EVALUATING THE EFFECT OF DMSO CONCENTRATIONS ON Y-GLOBIN EXPRESSION IN HULL CELLS,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 18, no. 2 (52), pp. 75–80, 2008, [Online]. Available: https://sid.ir/paper/98531/en

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