DETERMINATION OF ANTIGENIC DETERMINANTS IN THE C-TERMINAL 311 AMINO ACIDS OF HAPS ADHESION FROM NONTYPEABLE HAEMOPHILUS INFLUENZA

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TABATABAEE AKRAM; SIADAT SEYED DAVAR; MOUSAVI SEYED FAZOLLAH; AGHASADEGHI MOHAMMAD REZA

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Journal: MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY Year:2014 | Volume:24 | Issue:2 Page(s): 95-102

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Title

DETERMINATION OF ANTIGENIC DETERMINANTS IN THE C-TERMINAL 311 AMINO ACIDS OF HAPS ADHESION FROM NONTYPEABLE HAEMOPHILUS INFLUENZA

Author(s)

TABATABAEE AKRAM | SIADAT SEYED DAVAR | MOUSAVI SEYED FAZOLLAH | AGHASADEGHI MOHAMMAD REZA | Issue Writer Certificate

Keywords

NONTYPEABLE HAEMOPHILUS INFLUENZAEQ2

ANTIGENIC EPITOPESQ2

C-HAPSQ2

Abstract

Background: Haps protein plays central role in initial interaction of NONTYPEABLE HAEMOPHILUS INFLUENZAE (NTHi) with human respiratory epithelial cells. While other surface-exposed proteins of NTHi are highly variable, The HapS domain is highly conserved among H. influenzae strains. Recent studies demonstrated that HapS adhesive activity resides within the C-terminal 311 amino acids of the protein and also they showed that the C-terminal 311 amino acids of HapS (C-Haps) are capable of eliciting a protective immune response against NTHi colonization.Materials and methods: The pET24a-chaps plasmid harboring C-HAPS sequence from NTHi PTCC1766 was constructed. The amino acid sequences of C-HAPS of this study was aligned with C-HAPS of three NTHi strains (N187, TN106, P860295) and antigenicity plot of studied rC-Haps was done bioinformatic software. The pET24a-chaps expression was conducted in E.coli BL21 (D3E) and its expression was confirmed by SDS-PAGE and Western blotting methods. The rC-Haps was purified via immobilized metal affinity chromatography.Results: Amino acid sequence alignment of rCHaps sequence of current study and rC-Haps from the NTHi strains N187, TN106, P860295 showed more than %97 identity. Antigenicity plot identified 9 common highly antigenic domains that were located exactly in conserved regions among 4 different NTHi strains.Conclusion: Due to presence of highly conserved ANTIGENIC EPITOPES among C-HAPS of NTHi PTCC1766 and other NTHi strains, rC-Haps of current study could be theoretically a vaccine candidate against NTHi strains of different geographical areas.

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APA: Copy

TABATABAEE, AKRAM, SIADAT, SEYED DAVAR, MOUSAVI, SEYED FAZOLLAH, & AGHASADEGHI, MOHAMMAD REZA. (2014). DETERMINATION OF ANTIGENIC DETERMINANTS IN THE C-TERMINAL 311 AMINO ACIDS OF HAPS ADHESION FROM NONTYPEABLE HAEMOPHILUS INFLUENZA. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 24(2), 95-102. SID. https://sid.ir/paper/98535/en

Vancouver: Copy

TABATABAEE AKRAM, SIADAT SEYED DAVAR, MOUSAVI SEYED FAZOLLAH, AGHASADEGHI MOHAMMAD REZA. DETERMINATION OF ANTIGENIC DETERMINANTS IN THE C-TERMINAL 311 AMINO ACIDS OF HAPS ADHESION FROM NONTYPEABLE HAEMOPHILUS INFLUENZA. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2014;24(2):95-102. Available from: https://sid.ir/paper/98535/en

IEEE: Copy

AKRAM TABATABAEE, SEYED DAVAR SIADAT, SEYED FAZOLLAH MOUSAVI, and MOHAMMAD REZA AGHASADEGHI, “DETERMINATION OF ANTIGENIC DETERMINANTS IN THE C-TERMINAL 311 AMINO ACIDS OF HAPS ADHESION FROM NONTYPEABLE HAEMOPHILUS INFLUENZA,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 24, no. 2, pp. 95–102, 2014, [Online]. Available: https://sid.ir/paper/98535/en

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