RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION

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KOHAN ELHAM; SHAHHOSSEINY MOHAMMAD HASSAN; RAZAVI MOHAMMAD REZD; PARIVAR KAZEM; MOSLEMI ELHAM

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Journal: Medical Sciences Journal of Islamic Azad University,Tehran Medical Branch Year:2012 | Volume:22 | Issue:2 Page(s): 137-142

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Title

RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION

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Keywords

MYCOBACTERIUM TUBERCULOSIS COMPLEXQ2

RAPID DETECTIONQ2

LAMP TECHNIQUEQ2

PCRQ2

Abstract

Background: Rapid and efficient diagnosis is one of the crucial issues in the control of tuberculosis. Loop mediated isothermal amplification (LAMP) is a simple and rapid technique for nucleic acid amplification. In this study, we evaluate a LAMP assay targeting IS6110 gene for diagnosis of M. tuberculosis in clinical samples.Materials and Methods: 155 clinical sputum specimens were collected from suspected patients with tuberculosis. Six specific primers were designed for LAMP assay. LAMP reaction was performed under optimal condition. Sensitivity and specificity of LAMP were determined.Results: A successful LAMP reaction with newly designed primers was carried at 66oC for 60 minutes. The detection limit of LAMP was 5fg DNA per tube. The sensitivity and specificity of this assay in clinical sputum samples were 100% and 95.9%, respectively.Conclusion: LAMP is a rapid, sensitive and low cost technique that can be suitable for routine usage in diagnosis of TB.

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APA: Copy

KOHAN, ELHAM, SHAHHOSSEINY, MOHAMMAD HASSAN, RAZAVI, MOHAMMAD REZD, PARIVAR, KAZEM, & MOSLEMI, ELHAM. (2012). RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 22(2), 137-142. SID. https://sid.ir/paper/98722/en

Vancouver: Copy

KOHAN ELHAM, SHAHHOSSEINY MOHAMMAD HASSAN, RAZAVI MOHAMMAD REZD, PARIVAR KAZEM, MOSLEMI ELHAM. RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION. MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2012;22(2):137-142. Available from: https://sid.ir/paper/98722/en

IEEE: Copy

ELHAM KOHAN, MOHAMMAD HASSAN SHAHHOSSEINY, MOHAMMAD REZD RAZAVI, KAZEM PARIVAR, and ELHAM MOSLEMI, “RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION,” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 22, no. 2, pp. 137–142, 2012, [Online]. Available: https://sid.ir/paper/98722/en

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