CYTOTOXICITY EFFECT OF ICD-85 (VENOM – DERIVED PEPTIDES) ON PROMYELOCYTIC CANCER CELL LINES (HL-60)

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SHAHRAMYAR ZAHRA; ZARE MIRAKABADI ABBAS; MOROVATI HASSAN

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Journal: MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY Year:2012 | Volume:21 | Issue:4 (66) Page(s): 238-243

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Information Journal Paper

Title

CYTOTOXICITY EFFECT OF ICD-85 (VENOM – DERIVED PEPTIDES) ON PROMYELOCYTIC CANCER CELL LINES (HL-60)

Author(s)

SHAHRAMYAR ZAHRA | ZARE MIRAKABADI ABBAS | MOROVATI HASSAN | Issue Writer Certificate

Keywords

ANTICANCERQ2

PROMYELOCYTIC LEUKEMICQ2

ICD-85Q2

HL-60Q3

IC50Q2

VENOMQ2

MTT ASSAYQ2

CYTOTOXICITYQ2

Abstract

Background: Our previous studies revealed an inhibitory effect of ICD-85; a VENOM derived peptide has been extracted by researchers of Razi vaccine and serum research institute, on breast cancer cell line (MDA-MB231). The present study was undertaken to evaluate the anti proliferative effect of HL60 cells.Materials and methods: The effect of ICD-85 on proliferation of HL-60 cancer cells was determined using the MTT ASSAY. A quantitative CYTOTOXICITY assay based on the release of lactate dehydrogenase (LDH; EC 1.1.1.27) was performed 24 h after exposure to various concentrations of ICD-85. The morphological changes of ICD-85 treated HL-60 cancer cells were observed under electron microscope.Results: ICD-85 induced marked concentration inhibition of cancer cell proliferation with an IC50 value of 0.04µg/ml following 24 h incubation. Electron microscopic findings of ICD-85 treated cells showed nuclear material condensation, endoplasmic reticulum dilation, mitochondria swelling or degradation, increased cytoplasmic vacuoles, reduction or disappearance in cytoplasmic process and decreased nuclear/cytoplasmic ratio. LDH determination of cultured media of HL60 cells exposed to various concentration of ICD-85 revealed no significant changes in LDH levels compared with untreated cells.Conclusion: The results of present study indicated that ICD-85 inhibited the cancer cell proliferation by inducing apoptosis rather than necrosis.

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APA: Copy

SHAHRAMYAR, ZAHRA, ZARE MIRAKABADI, ABBAS, & MOROVATI, HASSAN. (2012). CYTOTOXICITY EFFECT OF ICD-85 (VENOM – DERIVED PEPTIDES) ON PROMYELOCYTIC CANCER CELL LINES (HL-60). MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, 21(4 (66)), 238-243. SID. https://sid.ir/paper/98887/en

Vancouver: Copy

SHAHRAMYAR ZAHRA, ZARE MIRAKABADI ABBAS, MOROVATI HASSAN. CYTOTOXICITY EFFECT OF ICD-85 (VENOM – DERIVED PEPTIDES) ON PROMYELOCYTIC CANCER CELL LINES (HL-60). MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY[Internet]. 2012;21(4 (66)):238-243. Available from: https://sid.ir/paper/98887/en

IEEE: Copy

ZAHRA SHAHRAMYAR, ABBAS ZARE MIRAKABADI, and HASSAN MOROVATI, “CYTOTOXICITY EFFECT OF ICD-85 (VENOM – DERIVED PEPTIDES) ON PROMYELOCYTIC CANCER CELL LINES (HL-60),” MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY, vol. 21, no. 4 (66), pp. 238–243, 2012, [Online]. Available: https://sid.ir/paper/98887/en

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