Laser Scanning Confocal Microscopy

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Hadian Parvin

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF LABORATORY KNOWLEDGE Year:2022 | Volume:10 | Issue:2 (38) Page(s): 19-24

Download Full-Text

Persian Verion

View:

309

Download:

Cites:

Information Journal Paper

Title

Laser Scanning Confocal Microscopy

Author(s)

Hadian Parvin | Issue Writer Certificate

Keywords

Laser scanning confocal microscopy

Fluorescence

Autofluorescence

Abstract

Confocal microscopy is an established light microscopical technique for imaging fluorescently labeled specimens with significant three-dimensional structure. Laser scanning confocal microscopy has become an invaluable tool for a wide range of investigations in the biological and medical sciences for imaging thin optical sections in living and fixed specimens ranging in thickness up to 100 micrometers. Modern instruments are equipped with 3-5 laser systems controlled by high-speed filters, which allow very precise regulation of wavelength and excitation intensity. Coupled with photomultipliers that have high quantum efficiency in the near-ultraviolet, visible and near-infrared spectral regions, these microscopes are capable of examining Fluorescence emission ranging from 400 to 750 nanometers. Instruments equipped with spectral imaging detection systems further refine the technique by enabling the examination and resolution of fluorophores with overlapping spectra as well as providing the ability to compensate for autoFluorescence. Recent advances in fluorophore design have led to improved synthetic and naturally occurring molecular probes, including fluorescent proteins and quantum dots, which exhibit a high level of photostability and target specificity.

Cites

No record.

References

No record.

Cite

APA: Copy

Hadian, Parvin. (2022). Laser Scanning Confocal Microscopy. IRANIAN JOURNAL OF LABORATORY KNOWLEDGE, 10(2 (38) ), 19-24. SID. https://sid.ir/paper/992889/en

Vancouver: Copy

Hadian Parvin. Laser Scanning Confocal Microscopy. IRANIAN JOURNAL OF LABORATORY KNOWLEDGE[Internet]. 2022;10(2 (38) ):19-24. Available from: https://sid.ir/paper/992889/en

IEEE: Copy

Parvin Hadian, “Laser Scanning Confocal Microscopy,” IRANIAN JOURNAL OF LABORATORY KNOWLEDGE, vol. 10, no. 2 (38) , pp. 19–24, 2022, [Online]. Available: https://sid.ir/paper/992889/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops