Sesame has valuable oil which is beneficial for human health. This plant has one capsule in each leaf node (the place where the leaf joins the stem), and by manipulating plant hormones, the number of capsules in each leaf node can increase, which leads to an increase in yield. To increase the number of capsules per leaf node, a factorial experiment was carried out in a randomized complete block design. The first factor was two types of seeds [seeds from one capsule per leaf node (CAP1) and seeds from triple capsule per leaf node (CAP2)], and the second factor was three concentrations of cytokinin (CK) (zero, 50, 100 ppm). The results showed that the maximum plant length (83.5 cm) was obtained from the 50 ppm CK treatment. The application of 50 ppm CK in CAP1 was increased the number of single capsule nodes about 48%. Compared with the control and 100 ppm treatments, the 50 ppm CK treatment had the greatest effect on the number of nodes with triple capsules (7.71), with increases of 38.6 and 72.8%, respectively. The greatest number of triple capsule nodes per plant (10.3) was obtained in the CAP2 treatment by using 50 ppm CK. The greatest amount of fresh and dry weight (84.5 and 30.7 g per plant, respectively) was obtained from the 50 ppm CK treatment group. The maximum number of capsules per plant and number of seeds per capsule were obtained from the 50 ppm CK×CAP2 treatment. Other results showed that the application of 50 ppm CK led to the highest number of seeds produced per plant (6.75 grams). In addition, the exogenous application of cytokinin to plants has the greatest effect on the areas to which it moves or the areas where cytokinin is synthesized, but to improve the effectiveness of this hormone and obtain an economic crop, it is necessary to use an appropriate ratio of other plant growth regulators.