COTTON, as a strategic crop, plays an important role in providing the human food and clothing needs due to oil, protein and fiber. Due to the high cost of COTTON production (due to the high number of working days required), the use of COTTON harvesting machines is in the interest of most farmers. In this study, the performance of COTTON picker in new COTTON cultivars was compared with each other. In this research, the efficiency of Performance of John Deere 9920 COTTON picker was investigated on five new COTTON cultivars Golestan, Armaqan, Kashmar, Sajedi and Khorshid. The project was implemented in a Randomized Complete Block Design with three replications and data was analyzed by using SAS software. The results indicated that there was a significant difference between the five cultivars in terms of machine harvest efficiency. Golestan cultivar had the best performance with an average of 87. 6 percent of total harvestable seed COTTON. Considering 8% of the residues per plant of this cultivar, the machine harvest efficiency will have reached to 95. 6 % if we could have used COTTON picker again.