An empirical electrical Conductivity assessment of nanofluids comprising CuO nanoparticles water-based in different concentrations, particles size and various temperatures of nanofluids has been carried out in this paper. These experimentations have been done in deionized water with nanoparticles sizes such as 89, 95, 100 and 112 nm and concentrations of 0.12 g/l, 0.14 g/l, 0.16 g/l and 0.18 g/l so nanofluids obtain in temperatures such as 25oC, 35oC, 45oC and 50oC for investigation of their electrical Conductivity. It is observed that, in water-based nanofluids, the electrical Conductivity increases with increasing in both nanofluids temperatures and concentration respectively in the range 25-50oC and 0.12-0.18g/l. But in nanoparticles size rising in nanofluids we observe that electrical Conductivity has a few increase when nanoparticles have 95nm diameters, so decrease for biggest nanoparticles such as 100 and 112nm. It seems that there is an optimum in electrical Conductivity with resize of nanoparticles.