Introduction: ere is probably a relationship between cancer and GABA, because systemic administration of baclofen, GABAB receptor agonist, significantly reduced the incidence and number of gastric cancers in rat. Moreover, GABA is accumulated in some malignant tissues and it level changes in brain tumors. It seems that increased GABA level could reflect a local anti-tumor response and also has a protective role against epileptic activities. The aim of this study was to assess GABA uptake in synaptosomes prepared from human glial tumors.Methods: In this study, human tumoral (n=11) and intact (if available) (n=14) brain specimens were obtained from patients during surgery. Synaptosome was prepared from samples (tumoral and intact) according to Raiteri et al method. Uptake was initiated by addition of the [3H] GABA and after incubation for 15 minutes at 37˚C, uptake was terminated by adding cold buffer. The samples were filtred under slight vacuum in superfusion system and washed twice with cold buffer. Filters radioactivity was determined in a scintillation counter. Independent t-test was used for comparison between the results of tumoral and intact groups.p values less than 0.05 was considered to be significant.  Results: We studied uptake of GABA in synaptosomal preparations from human tumoral and intact brain samples. Data showed a reduction in GABA uptake in tumoral compared with intact tissues (p<0.005).Conclusion: Following reduction in GABA uptake in brain tumors, brain extracellular concentration of GABA will increase. It suggests that elevation of extracellular GABA level may play important role in reduction of epileptic activity in brain tumors.