This article used an accurate urine sample (US) from a person with daily use of methadone (MTD), whose presence was confirmed through the diffusion liquid-liquid microextraction method followed by the gas chromatography-mass spectroscopy (GC-MS) technique. At a temperature of 40 0C, keep the sample. The 2nd generation polyamidoamine dendrimer (2-GPD), which was previously synthesized, was released in the US Samples were taken from the container at different intervals.It injected the model into the high-performance liquid chromatography ultraviolet (HPLC-UV) machine, filtering and extraction by diffusion liquid-liquid microextraction. Examining the HPLC-UV absorption of the samples taken from the US shows that with time MTD molecules migrate from the US into the cavities of the 2-GPD, and the ultraviolet absorption of MTD decreases. The finding confirms the suitability of 2-GPD as an agent for the extraction. The identification of MTD was reported in the actual US Monitoring and controlling the consumption of various drugs, such as MTD, is a challenging clinical and legal toxicology issue. Examining medications in a model such as the US always requires high accuracy. These methods include. The pre-purification stage is based on precision instruments.