Histological and histochemical studies on the pancreas of Goose (Anser albifrons) were carried out using special staining and light microscope. The pancreas in Goose is serous tubuloacinar gland having exocrine and endocrine part. Smooth muscle fibres were absent in capsule of pancreas. Acinar cells have bizonal shape. Intralobular ducts, Interlobular and main excretory ducts were present within parenchyma. The intralobular ducts were lined with a simple cuboidal epithelium reach interlobular ducts lined with low columnar epithelium. The main excretory ducts were lined by simple to stratified columnar epithelium. The glands inside the connective tissue of the ducts and basophilic staining on the apical surface of pancreatic duct system were found from the interlobular ducts to the main excretory ducts. The endocrine part was consisted of various shapes and sizes of alpha and beta islets. Mixed islets were not observed in the Goose pancreas. Parasympathetic ganglia were observed in the exocrine pancreas. No significant differences were noted between males and females.

Background: Salmonella is one of the most important enteric pathogenic bacteria that threatened poultry health. Aims: This study aimed to investigate the prevalence and antimicrobial resistance of Salmonella isolates in Goose farms. Methods: A total of 244 cloacal swabs were collected from Goose farms to detect Salmonella in Northeast China. Antimicrobial susceptibility, and resistance gene distribution of Salmonella isolates were investigated. Results: Twenty-one Salmonella isolates were identified. Overall prevalence of Salmonella in the present study was 8. 6%. Among the Salmonella isolates, the highest resistance frequencies belonged to amoxicillin (AMX) (85. 7%), tetracycline (TET) and trimethoprim/sulfamethoxazole (SXT) (81%), followed by chloramphenicol (CHL) (76. 2%), florfenicol (FLO) (71. 4%), kanamycin (KAN) (47. 6%), and gentamycin (GEN) (38. 1%). Meanwhile, only 4. 8% of the isolates were resistant to ciprofloxacin (CIP) and cefotaxime (CTX). None of the isolates was resistant to cefoperazone (CFP) and colistin B (CLB). Twenty isolates (95%) were simultaneously resistant to at least two antimicrobials. Ten resistance genes were detected among which the blaTEM-1, cmlA, aac(6’ )-Ib-cr, sul1, sul2, sul3, and mcr-1. 1 were the most prevalent, and presented in all 21 isolates followed by tetB (20/21), qnrB (19/21), and floR (15/21). Conclusion: Results indicated that Salmonella isolates from Goose farms in Northeast China exhibited multi-drug resistance (MDR), harboring multiple antimicrobial resistance genes. Our results will be useful to design prevention and therapeutic strategies against Salmonella infection in Goose farms.

China's burgeoning animal husbandry sector has witnessed a notable expansion in Goose farming. Among the various health challenges, a novel Goose astrovirus (GoAstV) has emerged as a significant threat to the industry, necessitating prompt detection strategies to mitigate its economic impact. This research introduces a novel detection approach using real-time fluorescence-based reverse transcription recombinase-aided amplification (RT-RAA), offering a rapid and reliable method for GoAstV identification. We meticulously designed specific primers and probes, and optimized the RT-RAA reaction conditions. The assay's specificity, sensitivity, repeatability, and clinical efficacy were rigorously assessed. Our method achieves detection within a swift 26-min window at a constant temperature of 39.00 ˚C, boasting a detection threshold as low as 1.19 × 102 copies per μL. Notably, the assay exhibited no cross-reactivity with closely related viruses, including Newcastle disease virus, avian influenza virus H9 subtype, Goose circovirus, Goose parvovirus, duck Tembusu virus, and avian adenovirus type 4. Validation through testing of 40 clinical samples confirmed a 100% agreement with pre-existing data. The study's outcomes underscore the high specificity, sensitivity, and operational simplicity of the developed RT-RAA assay, positioning it as an ideal candidate for the rapid and on-site detection of GoAstV.