Aim: The present study aimed at investigating the necessity of preoperative Liver Function Tests (LFTs) in patients with uncomplicated gallstone disease before laparoscopic cholecystectomy. Background: Significant relationship between common bile duct (CBD) stones and acute cholecystitis is reported. There are contradictory reports about the effect of CBD stones on Liver Function Tests in patients (LFTs). Methods: In the current study, patients with symptomatic cholelithiasis who referred to hospitals during January 2015 and May 2016 were enrolled. Routine Tests and ultrasonography were performed on all patients before surgery. Data were presented as means ± SD and qualitative variables as frequency (percentage) were considered. Statistical analyzes were performed with SPSS software. Results: A consecutive series of 270 patients (58 males and 212 females) who referred for laparoscopic cholecystectomy were enrolled in this retrospective study. Pre-operative LFTs were normal in 249 patients (85%) and abnormal LFT was detected in 41 patients (15 %). Conclusion: This study showed that 15 % of patients with cholelithiasis without dilated CBD had impaired LFTs. Routine LFTs in preoperative evaluation of symptomatic cholelithiasis usually reveals normal findings and is not helpful in uncomplicated cholelithiasis.

Background: Although the activity of the cytosolic hepatic enzyme phenylalanine hydroxylase is impaired in phenylketonuria (PKU), the microsomal Function, necessary for the metabolism of xenobiotics, has never been assessed. The aim of the study was to assess the microsomal Liver Function in PKU patients by 13C-methacetin breath Test (13C-MBT), which measures cytochrome P450 activity. Methods: Twenty patients with classical PKU, aged 15-34 years, were enrolled in the study. The control group comprised of 20 healthy subjects matched for age and sex. The 13C-MBT was performed in every subject at rest after an overnight fast. Results: The values of cumulative percentage dose recovery at 120 minutes of the Test (CPDR median < interquartile range >) were significantly lower in the PKU group than in the control group (25. 1 < 21. 8-28. 83 > vs 32. 8 < 27. 4-34. 1 >; P = 0. 0067). The lower values of the maximum momentary 13C recovery (Dmax) were observed in the PKU group than in the control group (26. 6 < 22. 4-28. 7 > vs. 32. 1 < 27. 18-34. 08 >; P = 0. 0227). The values of time to reach Dmax (Tmax) did not differ (20 < 17-20 > vs. 20 < 20-20 >; P = 0. 8410). The values of CPDR, Dmax and Tmax were not different between the group with abnormal (> 10 mg/dL) and normal (< 10 mg/dL) Phe level (P = 0. 2799; 0. 7959; 0. 4812, respectively). Conclusions: The present study demonstrates that the cytochrome P450 activity in PKU patients may be reduced. However, further research is required, particularly in regards to the effect that patient diet compliance has on the microsomal Liver Function.

Background: Celiac disease (CD) has been found in up to 10% of the patients presenting with unexplained abnormal Liver Function Tests (LFT). As there is no precise data from our country in this regard, we investigated the prevalence of CD in patients presenting with abnormal LFT.Methods: From 2003 to 2008, we measured IgA anti-tissue transglutaminase (t-TG) antibody (with ELISA technique) within the first-level screening steps for all patients presenting with abnormal LFT to three outpatient gastroenterology clinics in Isfahan, IRAN. All subjects with an IgA anti-tTG antibody value of >10 m/ml (seropositive) were undergone upper gastroinTestinal endoscopy and duodenal biopsy. Histopathological changes were assessed according to the Marsh classification. CD was defined as being seropositive with Marsh I or above in histopathology and having a good response to gluten free diet (GFD).Results: During the study, 224 patients were evaluated, out of which, 10 patients (4.4%) were seropositive for CD. Duodenal biopsies were performed in eight patients and revealed six (2.7%) cases of Marsh I or above (four Marsh IIIA, two Marsh I), all of them had good response to GFD. The overall prevalence of CD among patients with hypertransaminasemia, autoimmune hepatitis, and cryptogenic cirrhosis was determined as 10.7% (3/28), 3.4% (2/59), and 5.3% (1/19), respectively.Conclusion: Serological screening with IgA anti-tTG antibody Test should be routinely performed in patients presenting with abnormal LFT and especially those with chronic Liver diseases including hypertransaminasemia, autoimmune hepatitis, and cryptogenic cirrhosis.

Background: Asphalt is a mixture of aliphatic and aromatic hydrocarbons. It also contains different kinds of heavy metals. Hot mix asphalt emits hazardous volatile substances. Asphalt is used in many industries, causing serious adverse health effects for workers. Therefore, investigation of the health status of workers is of crucial importance. The present study aimed to assess the impact of asphalt vapor on hepatic and renal Function Tests parameters and the clinical parameters in road paving workers compared to healthy subjects. Methods: In a cross-sectional study, 41 road asphalt paving workers and 120 healthy nonexposed subjects were selected. The serum enzymes of Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), total bilirubin, triglyceride, urea, and creatinine levels were assessed using diagnostic kits. A medical practitioner examined all subjects for any possible medical condition. Results: Liver and kidney Function Test parameters were significantly higher in road asphalt paving workers compared to their healthy counterparts (P<0. 05 for all parameters). About 63% of workers exposed to asphalt fumes for more than 10 years showed cardiac, pulmonary, and metabolic complications. Conclusion: Significant elevations in Liver and kidney Function Test parameters suggest that exposure to asphalt fumes is associated with hepatic and renal dysFunction in asphalt-exposed workers.

Liver Function Tests are frequently used to screen Liver Function, monitor therapy, and determine the severity of Liver problems. The present study aimed to assess the consistency of the results of the Liver Function parameters between the two analyzers, Architect c8000 and Cobas C501. This laboratory-based analytical observational study was conducted in a cross-sectional manner. Sample collection was performed through a consecutive sampling procedure from June to December 2019 in the Clinical Pathology Laboratory, Dr. Mohammad Hoesin General Hospital, Palembang, South Sumatra, Indonesia. The research sample consisted of the Liver Function examination results of patients, carried out using the Architect c8000 and Roche Cobas c501 chemistry analyzers. Serum albumin, alanine transaminase, aspartate aminotransferase, and total protein were the studied variables. The Spearman, Mann-Whitney, and Bland-Altman Tests were used to evaluate the comparison Test. In total, 100 blood samples were obtained in this study. The results revealed a highly significant correlation (r>0.90, P=0<001) among the four Liver Function parameters. The results of the Liver Function parameters inspected by the two analyzers did not differ significantly (P>0.05). In addition, there was a solid agreement on all parameters, with a near-perfect level (concordance correlation coefficient>0.90) and more than 95% of data points falling within the acceptable range. The Architect c8000 and Cobas c501 analyzers produced similar results for Liver Function Tests; hence, these devices can be used interchangeably.