The aim of this study was a strain-improvement program for Trichoderma reesei PTCC 5142 by using a combination of UV light and NTG (N-methyl-N'-nitro-N-nitrosoguanidine) for enhanced cellulase production. Following mutagenesis after several rounds, mutant A6: 2 was selected from a total of 6500 colonies. Results obtained after 4 days were: Enzyme activity 1.26 U/ml and 0.82 U/ml for exoglucanase and endoglucanase, respectively. The comparative results showed increased production exoglucanase and endoglucanase by mutant A6: 2 than Trichoderma reesei PTCC 5142 to amount 130% for exoglucanase and 156% for endoglucanase.

The PageRank algorithm is one of the web-based classification methods used by Google search engine first. The main purpose of this algorithm was to determine the popularity of Web pages. The algorithm uses the web links structure to find important pages. One of the problems of PageRank and the same algorithms based on the web graph is that the number of a page is propagated to its output pages without any control while the output screen really is not really recommended by the previous page directly or indirectly. In this paper, by changing the original formula PageRank, a method has been proposed to prevent the entry of the input bonds to a single page of publication without gaining popularity. In order to evaluate the proposed algorithm, a single web graph is constructed, which in some nodes has a rating leap. This mutation decreases after the proposed algorithm is applied.

Introduction ore than 20 years have elapsed since the first single base pair substitution underlying an inherited disease in humans was characterized at the DNA level. Disease-associated gene lesions are currently collected and publicized by the Human Gene Mutation Database (HGMD) in Cardiff, locusspecific mutation databases, and to some extent by the Genome Database (GDB) and Online Mendelian Inheritance in Man (OMIM) as comprehensive and up-to-date resources for information on genetic disorders and mutations causing them.1 – 4

Objective(s): Denaturing high performance liquid chromatography (DHPLC) is a high throughput approach for screening DNA sequence variations. To assess oven calibration, cartridge performance, buffer composition and stability, the WAVE Low and High Range Mutation Standards are employed to ensure reproducibility and accuracy of the chromatographic analysis. The purpose of this study was to provide a cost-effective homemade mutation standard for DHPLC analysis.Materials and Methods: DHPLC was performed to evaluate different elution temperatures of a 374 bp DNA fragment with C>A mutation at position of 59 to achieve a peak profile similar to the Low Mutation Standard. In order to verify the reproducibility of the homemade mutation standard using DHPLC, 15 different experiments were performed to compare the homemade mutation standard, the WAVE Low Range Mutation Standard with a positive DNA control sample.Results: We identified a comparable elution temperature and a peak profile with the WAVE Low Range Mutation Standard.Conclusion: This study confirmed the reproducibility of the peak profile of our homemade mutation standard compared to the Low Mutation Standard using DHPLC analysis.