Introduction: Endodontic instruments are developed to provide a better cleaning of the root canal system and reduce its risk of fracture. The aim of this study was to evaluate the instrumentation time and cyclic fatigue resistance of WaveOne Gold, ProDesign R and ProDesign Logic files. Methods and Materials: Thirty Nickel-titanium (NiTi) rotary instruments were divided into 3 groups (n=10). ProDesign Logic file 25/0. 06 was used in continuous rotation after glide path preparation. WaveOne Gold 25/0. 07 and ProDesign R 25/0. 06 files were used in reciprocating motion. Every file instrumented 3 standardized artificial canals. The average time, the number of cycles (NCI) and cyclic fatigue resistance of each file were determined through the number of cycles to failure (NCF) in a stainless-steel device. The total amount of cycles to fracture was also calculated (NCI+NCF). Data was analyzed using the Kruskal-Wallis and Mann-Whitney tests. Results: The instrumentation time of the ProDesign Logic file was significantly lower when compared to the other files (P=0. 019). The longest times to failure were presented by ProDesign Logic (182. 07 sec) and ProDesign R (152. 38 sec) files. The same differences were observed for the NCF (910. 37 and 761. 93). The WaveOne Gold group presented a lower NCF as well as a smaller sum of NCI+NCF (748. 33) that was statistically significant when compared to the other groups (P<0. 05) respectively. Conclusion: The use of continuous rotational motion in canals with a glide path in the ProDesign Logic group led to shorter instrumentation time. The cyclic fatigue resistance of ProDesign R and Logic instruments was superior to WaveOne Gold. The thermal treatment of the instrument’ s alloy, its cross section and the glide path seems to influence the cyclic fatigue resistance.