The aim of current study is investigation effectiveness of 3 months aerobic exercise with Origanum majorana extract on male rats soleus muscle apoptosis. In current experimental study, 35 heads of male rats with 3 month age were alternated randomize in 5 groups( aerobic exercise, Origanum majorana extract, exercise+ Origanum majorana extract, 6 months controlling and also 3 months controlling. Exercise and exercise+ Origanum majorana extract groups implemented aerobic exercises during 12 weeks (5 days per week, 15% inclination, 10-60 minutes and 24-33 m/m speed). After 48 hours of last exercise, male rats soleus muscle were extracted and involved genes expression in apoptosis (Caspase 9 and Cythocrome C) were investigated by PCR. Data were analyzed by one-way variance analysis test at 0. 05 significant levels. Results showed that, after intervention; soleus muscle weight and its proportion against body weight had significant difference (P<0. 05) between groups. Also caspase 9 relative expression amount between groups had significant difference (P<0. 05) after intervention, but there wasn't significant difference between groups according to Cythocrome C relative expression amount. (P>0. 05) Aging cause to increasing rats body weight and reducing soleus muscle weight against body weight and also Caspase 9 increase simultaneously, but Cythocrome C expression amount doesn’ t change. So, exercise and consumption of Origanum majorana extract lower mentioned increasing cases.