Background and Objectives: Carbapenem-resistant Acinetobacter baumannii has recently been identified by the World Health Organization as a critical pathogen. We propose to characterize the molecular characteristics of clinical isolates of A. baumannii resistant to carbapenems collected in a Moroccan hospital. Materials and Methods: Seventy carbapenem-resistant A. baumannii isolates from various samples were received at the mi-crobiology laboratory of the Hospital Center. Antibiotic susceptibility was tested by the diffusion disc method and molecular characterization of antimicrobial resistance was performed by PCR and sequencing. Results: Carbapenemase genes were detected in our isolates: the OXA-51 gene and the ISbA1 sequence were detected in all isolates (100%), the OXA-23 and OXA-58 genes were detected in 82. 85% and 10% of isolates respectively, MBL genes were dominated by VIM 39 isolates (55. 7%), followed by GIM 26 isolates (37%), SIM 20 isolates (28. 5%), IMP 8 isolates (11, 4%), NDM 3 isolates (4%) and for the first time in Morocco SPM with 4 isolates (5. 7%). Conclusion: The emergence of resistance of A. baumannii to carbapenems is a serious problem in our hospital which re-quires the establishment of a prevention strategy and strict respect for hygiene to minimize their dissemination.