Objective: Omega-3 is found in high levels in mammalian spermatozoa and it could improve sperm classical parameters that correlate closely with fertilization. The aim of this study was to evaluate the apoptosis incidence andin vitro fertilization (IVF) rate following ram diets supplementation with omega-3 source.Materials and Methods: Eight of 2-3 years old Zandi rams (live weight: 65±10kg) was randomly assigned into two groups: control (CTR) and fish oil (FO). Energy, protein and vitamin E supplement level was similar in both groups. Rams in FO group received maintenance diet in associated with 35gr/day/ram fish oil. diet were fed for 70 days. After adaptation period (21 d), semen was collected from each ram by artificial vagina and transferred to laboratory. Fertilization capacity of swimup processed semen was only assessed at the first and last day of sampling byin vitro fertilization technique, but apoptosis incidence was evaluated in every week of sampling by flow cytometry technique.Results: Flow cytometry results showed that totally the occurrence of apoptosis in the FO group was lower than CTR group. This difference was only significant in the 3 rd week of sampling (p≤0.05). Also two cell stage embryos rate following IVF in two groups indicated significant higher rate in FO group as compared with the CTR group (p≤0.05).Conclusion: Overall, it could be concluded that adding fish oil to rams diets not only, could reduce the Probability of apoptosis incidence, but also could increase the fertilization rate. It seems that omega-3 could be an appropriate additive in ram diet.

Background & Objective: The effect of storage time and temperature on the prepared semen sample was evaluated, but the optimal condition is unclear. The aim of this study was to assess the effect of long-term incubation of prepared sperm at testicular temperature versus room temperature on semen parameters and DNA fragmentation index (DFI). Materials & Methods: Sperm samples were collected from 40 patients between 2019 and 2020. Each sample was separated into two parts and underwent a non-direct swimup method. One group was placed in a 35°, C incubator, and the other group was kept at room temperature (26°, C) in the dark. Both groups were evaluated at intervals of 45 minutes, 24 hours and 48 hours after sampling in terms of sperm concentration, motility, morphology, and DFI. Student t-test and repeated measures analysis of variance were used. Results: Sperm count (P=0. 007) and motility (P<0. 001) at 26°, C in three-time intervals of 45 minutes, 24 hours and 48 hours were significantly higher than 35°, C. The proportion of normal morphology spermatozoa at 26 and 35°, C at 45 min, 24 h, and 48 h did not show a significant difference (P=0. 08). DFI at 26°, C in three-time intervals was significantly lower than 35°, C (P=0. 008). Conclusion: The results of this study indicated that when the prepared sperm samples are incubated for 24 h at 26°, C compared to 35°, C, they show significantly better quality and good quality of sperm can be retained for several hours if stored at room temperature.

Background: The health effect of rapidly increasing everyday exposure of humans to radiofrequency radia on is a major global concern. Mobile phone jammers prevent the mobile phones from receiving signals from base sta ons by interfering with authorized mobile carriers’ services. In spite of the fact that mobile jammer use is illegal, they are occasionally used in offices, shrines, conference rooms and cinemas. The purpose of this study was to inves gate the biological effects of short term exposure of human sperm to radiofrequency radia on emi$ed from a commercial mobile phone jammer. Materials and Methods: Fresh semen samples were obtained by masturba on from 50 healthy donors who had referred with their wives to Infer lity Treatment Center at the Mother and Child Hospital, Shiraz University of Medical Sciences. Female problem was diagnosed as the reason for infer lity in these couples. The semen sample of each par cipant was divided into 4 aliquots. The first aliquot was subjected to SWIM-UP and exposed to jammer radia on. The second aliquot was not subjected to swimup but was exposed to jammer radia on. The third and fourth aliquots were not exposed to jammer radia on but only the 3rd aliquot was subjected to SWIM-UP. Results: Semen samples exposed to radiofrequency radia on showed a significant decrease in sperm mo lity and increase in DNA fragmenta on. Conclusion: Electromagne c radia on in radiofrequency range emi$ed from mobile phone jammers may lead to decreased mo lity and increased DNA fragmenta on in human semen. It can be concluded that mobile phone jamming might exert adverse reproduc ve health effects.