The current study aimed to evaluate the role and gene target of miR-126 in Iraqi patients with Coronary Artery Disease (CAD) and atherosclerosis (AS). By coverage average age (35 to 65) years old of both genders, a total of 40 patients with CAD and 40 healthy people as a control group were enrolled in this clinical study. The blood samples of patients were taken from Ibn Al-Bitar Diagnostic Center for Heart Surgery in Baghdad/Iraq in December 2020 to April 2021. Overall, the CAD patients had significantly higher (except HDL) total lipid profile (total chol., total Trig., LDL, and VLDL, age, and IL6 levels). Statistically no significant correlation was found between miRNA-126 and lipid parameters in all studied subject. The correlation was found between Triglycerides and his-Troponin (r=-0.521, pv=0.032). The ROC analysis of mRNA126 at UAC 0.976 had sensitivity of (0.80) and 1-specificity of (0.60) were total area under the curve = 759, pv=0.005; that means miRN-126 can be used as a screening test for CAD. Thus, miR126 can be considered as a biomarker on CAD in patient.