INTERNATIONAL IRANIAN BIOLOGY CONFERENCE | Year:2012 | دوره:17 |Papers Count:74

BACKGROUND AND AIM: THE ANTIBIOTICS’ SIDE EFFECTS AND MICROBIAL RESISTANCE HAVE INCREASED ATTENTION TO NATURAL ANTIMICROBIAL TO TREAT INFECTIONS. THIS STUDY WAS AIMED TO INVESTIGATE THE ANTIMICROBIAL EFFECTS BLACK SEED (NIGELLA SATIVA) EXTRACT AND ITS POSSIBLE NEPHROTOXICITY IN COMPARISON TO GENTAMICIN. THE EFFECT OF BLACK SEED ON GENTAMICIN INDUCED RENAL TOXICITY AND ITS SYNERGISTIC EFFECT ON ANTIBACTERIAL ACTIVITY OF GENTAMICIN WAS ALSO EVALUATED….

L-SERIN IS AN AMINO ACID. IT WAS ISOLATED FIRST THE SILK PROTEIN NAMED SEVICIN IN 1865.IT HAS SIX CODON WHICH ARE AGU; AGC; UCC; UCU; UCG; UCA. AMONG MANY METHODS FOR DETERMINATION OF L-SERIN IN...

FISHMEAL IS AN IMPORTANT COMMERCIAL PRODUCT THAT IS OBTAINED BY PROCESSING THE BONES AND WHOLE FISH.DETECTION OF ADULTERATION IN FISHMEAL WITH OTHER MEATS IS IMPORTANT FOR LIVESTOCK AND POULTRY PRODUCTION AND THEIR HEALTHY. THE AIM OF THIS STUDY WAS TO IDENTIFICATION OF FRAUD AND ADULTERATION IN FISHMEAL PRODUCTS.124 FISHMEAL SAMPLES WERE COLLECTED FROM MANUFACTURERS AND EXAMINED FOR PRESENCE OF POULTRY AND RUMINANTS MEATS. TOTAL DNA WAS EXTRACTED FROM FISHMEAL SAMPLES AND PCR WAS PERFORMED FOR GENE AMPLIFICATION OF MEAT SPECIES. OUT OF 124 FISHMEAL PRODUCTS EXAMINED 9 (7.25%), 4 (3.22%) AND 16 (12.9%) SAMPLES CONTAMINATED WITH BOVINE, SHEEP AND CHICKEN, RESPECTIVELY. THE PCR IS AN EFFECTIVE AND RAPID TECHNIQUE WHIT HIGH ACCURACY THAT CAN BE USED TO DETECT AND PREVENT OF THE FISHMEAL ADULTERATIONS.

FACTOR IX IS A PLASMA GLYCOPROTEIN NECESSARY FOR THE BLOOD COAGULATION PATHWAY, FOR WHICHΓ-CARBOXYLATION IS CONSIDERED AS A CRITICAL POST TRANSLATION MODIFICATION, THAT IS REQUIRED FOR ITS BIOLOGICAL ACTIVITY. DUE TO THE FACT THAT THE AFFINITY OF THE PROTHROMBIN PROPEPTIDE FOR THEΓ-CARBOXYLASE IS LESS THAN THE FACTOR IX PROPEPTIDE...

RAPID DETECTION OF THE INFLUENZA VIRUSES IS BASIC MEANS FOR PREVENTING WIDESPREAD TRANSMISSION. IN THIS STUDY A TAQMAN REAL-TIME PCR WAS COMPARED WITHPCR ASSAY FOR IDENTIFICATION OF H9N2 INFLUENZA VIRUSES. A TOTAL OF 21 CHICKEN'S FECAL SAMPLES WERE ANALYZED FOR H9N2 INFLUENZA GENOME. OVERALL, 15 AND 12 SAMPLES WERE POSITIVE IN THE REAL-TIME PCR AND PCR ASSAYS, RESPECTIVELY.THE AMPLIFIED VIRUS GENOME WAS DETECTED BY REAL-TIMEPCR WITHIN 2 HOURS COMPARED TO 3 HOURS WITH PCR ASSAY. THE RESULTS SHOWED THAT THE REAL-TIME PCRHAD A HIGHER PERFORMANCE REGARDING THE DETECTION OF INFLUENZA AS COMPARED TO THE PCR METHOD.

MITOCHONDRIAL DNA (MTDNA) COULD BE CONSIDERED A CANDIDATE MODIFIER FACTOR FOR SYNDROME OF LONG QT (LQTS), SINCE MITOCHONDRIAL OXIDATIVE STRESS IS THOUGHT TO BE INVOLVED IN ATP PRODUCTION. IT HAS BEEN REPORTED THAT THE ACTIVITY OF ION CHANNELS IN CARDIOMYOCYTES IS SENSITIVE TO ATP LEVEL. WE SEARCHED 40% OF THE ENTIRE MITOCHONDRIAL GENOME IN AN IRANIAN FAMILY WITH LQT3 FOR MUTATION BY PCR-SSCP AND DNA SEQUENCING. WE REPORT FOUR NEW MUTATIONS AND ONE REPORTED MUTATION, LEADING TO AN AMINO ACID SUBSTITUTION AND TWO MUTATIONS IN MITOCHONDRIAL TRNA. WE FOUND STATISTICALLY SIGNIFICANT CORRELATION (R=0.737) BETWEEN QTC (MS) AND AGE OF LQTS PATIENTS. OUR DATA SUGGEST THAT THESE MITOCHONDRIAL MUTATIONS IN A FAMILY WITH LQTS MIGHT BE RESPONSIBLE MITOCHONDRIAL DEFECTS AND INCREASE THE GRAVITY OF SYNDROME OF LONG QT (LQTS).

DURING CLEANING OF STORAGE TANKS FOR CRUDE OIL AND ITS DERIVATIVES, LARGE VOLUMES OF OILY SLUDGE ARE DISCHARGED TO THE ENVIRONMENT. THEREFORE, IT CAN CAUSE ENVIRONMENTAL CONTAMINATION PARTICULARLY SOIL POLLUTION. REPORTS INDICATE THAT MANY COMPONENTS OF OILY SLUDGE ARE CARCINOGENIC. IN THIS REGARD, A VARIETY OF PHYSICAL, CHEMICAL AND BIOLOGICAL REMEDIATION TECHNIQUES MAY BE USED...

TWO UN-STRUCTURED KINETIC MODEL FOR RH-GCSF PRODUCTION BYESCHERICHIA COLI IN A FED BATCH REACTOR ARE PROPOSED. ONE OF THEM PREDICTS PRODUCTION OF RH-GCSF BEFORE INDUCTION WHEN CONCENTRATION OF GLUCOSE DECREASES. THE OTHER ONE PREDICTS PRODUCTION OF RH-GCSF AFTER INDUCTION. WE CALCULATE PARAMETERS OF THE KINETIC MODELS BY FITTING MODELS TO EXPERIMENTAL DATA. THESE EXPERIMENTAL DATA HAVE BEEN OBTAINED IN EXPERIMENTS IN A FED BATCH REACTOR. SUGGESTED MODELS ARE UNSTRUCTURED NONSEGREGATED. R2 OF THE MODEL THAT PREDICTS PRODUCTION BEFORE INDUCTION IS EQUAL TO 0.9356 AND AFTER INDUCTION IS ABOUT 0.9966.

TRYPTOPHAN IS AN AMINO ACID NEEDED FOR NORMAL GROWTH IN FANTHS AND FOR NITROGEN BALANCE IN ADULTS. IT IS ENCODED IN THE STANDARD GENETIC CODE AS THE CODON UGG. AMONY MANY METHODS FOR DETERMINATION OF TRYPTOPHAN IN BIOLOGICAL SAMPLES...

KINDLING IS A MODEL OF EPILEPSY WHICH RESULTS IN CHANGES OF SYNAPTIC PLASTICITY DUE TO REPETITIVE ELECTRICAL OR CHEMICAL STIMULATION OF THE BRAIN. PLASTICITY CHANGES CAN LEAD TO STRUCTURAL ALTERATION IN EPILEPTIC SUSCEPTIBLE AREAS LIKE HIPPOCAMPUS...

INTERGENERIC HYBRIDIZATION IS ONE WAY TO TRANSFERRING DESIRABLE GENE/S INTO FIELD CROPS. IN ORDER TO IMPROVE PRIMARY TRITIPYRUMLINES BY D GENOME OF BREAD WHEAT (TRITICUM AESTIVUM, 2N=6X=42, AABBDD). THE VARIOUS 167 CROSSES BETWEEN 6X BREAD WHEAT CULTIVARS (AABBDD) AND PRIMARYTRITIPYRUM LINES (2N=6X=42, AABBEBEB) WAS CARRIED OUT AND 49 F1 SEEDS WAS PRODUCED.

THE ICHNEUMONIDAE IS A LARGE FAMILY OF HYMENOPTERA INCLUDING A VAST NUMBER OF PARASITIC WASP SPECIES. A SURVEY WAS PERFORMED ON OCCURRENCE OF THE ICHENUMONIDS WASPS BELONG TO SUBFAMILIES, ANOMALONINAE AND OPHIONINAE AT SISTAN & BALUCHESTAN PROVINCE DURING 2010-2011. THE SPECIMENS WERE COLLECTED FROM DIFFERENT LOCALITY USING STANDARD SWEEPING NET. SIX SPECIES WERE COLLECTED AND IDENTIFIED, OF WHICHBARYLYPA RUFA (HOLMGREN, 1857) (ANOMALONINAE) IS A NEW RECORD FOR THE FAUNA OF IRAN. THREE SPECIES CONSISTINGANOMALON CRUENTATUM (GEOFFREY, 1828), BARYLYPA RUFA (HOLMGREN, 1857) (ANOMALONINAE) AND ENICOSPILUS STENOPSIS(KOHL, 1905) (OPHIONINAE) ARE NEWLY RECORDED FROM SISTAN & BALUCHESTAN PROVINCE.

ANAMORPHIC FUNGI ARE A CONTROVERSIAL GROUP AMONG FUNGI AND THEIR CLASSIFICATION CRITERIA HAS UNDERGONE MANY CHANGES OVER THE YEARS. THE STUDY WAS CONDUCTED TO INVESTIGATE PHYLOGENETIC RELATIONSHIPS OF SOME IMPERFECT FUNGI. FOR THIS PURPOSE, SELECTED ISOLATES FROM PLANT TISSUES AND SOIL SAMPLES WERE COLLECTED FROM SEVERAL PROVINCES OF IRAN. THEN, THEIR TOTAL DNA WERE AMPLIFIED BY ITS AND TEF PRIMERS.PHYLOGENETIC RELATIONSHIP WHICH WAS CONDUCTED BY NEIGHBOR JOINING METHOD REVEALED THAT ITS CANT SEPARATE ALTERNARIA, ULOCLADIUM AND SOME CLADOSPORIUM SPECIES BUT EF-1A IS SUITABLE FOR BREAKING THEM UP.RESULTS OBTAINED FROM BOTH ITS AND EF-1A WAS IN CONGRUENT WITH EACH OTHER AND WITH PREVIOUSLY DESCRIBED PHYLOGENIES AND MORPHOLOGY BASED CLASSIFICATION SCHEMES.

THE AIM OF THIS STUDY WAS TO STUDY TOXICITY OF ORGANOPHOSPHATE (OP) PESTICIDES IN EXPOSED FARMERS IN TERMS OF NEUROCOGNITIVE IMPAIRMENT, MENTAL HEALTH STATUS. A COMPARATIVE CROSS-SECTIONAL ANALYSIS WAS DONE IN 174 FARMERS WHO HAD BEEN EXPOSED TO OPS IN COMPARISON TO A CONTROL GROUP CONTAINING 174 HEALTHY SUBJECTS WITH THE SAME AGE AND SEX AND EDUCATION LEVEL. THE NEUROCOGNITIVE IMPAIRMENT AND PSYCHOLOGICAL DISORDERS WERE SIGNIFICANTLY RAISED IN WORKERS. THE PRESENT FINDINGS INDICATE THAT INHIBITION OF ACHE CAN BE INCIDENCE OF NEUROPSYCHOLOGICAL DISORDERS.

THE PRESENT STUDY IS CONDUCTED TO IMPROVE THE KNOWLEDGE OF COMBINATION BREAST CANCER THERAPY.THE AIM OF THE PRESENT STUDY WAS TO DETERMINE THE EFFECT OF THE COMBINATION OF TAMOXIFEN AND TRANILAST ON THE HUMAN BREAST CANCER CELL LINE MCF-7...

BACKGROUND: HAP TENS WITH CARRIER MOLECULE WHICH ARE IN PROTEIN TYPE CAN BE AS A STRONG IMMUNOGENIC FACTOR OF ALLERGIES. LIMONENE ESSENCE IS FROM MONOTERPENOIDE GROUP THAT EXTRACTED FROM PLANT'S PARTS AND ORGANELLE APPLIED IN PHARMACEUTICAL AND PRODUCES...

ONE OF THE MAIN POST-TRANSCRIPTIONAL REGULATORS OF GENE EXPRESSION WITHIN A CELL ARE MICRORNAS. WHEN A MICRORNA BINDS TO ITS COMPLIMENT SEQUENCE ON TARGET MRNA, DEPENDING ON THE DEGREE OF BASE-PAIRING, TRANSLATIONAL INHIBITION OR TARGET TRANSCRIPT CLEAVAGE OCCURS...

LACCASES BELONG TO A FAMILY OF MULTICOPPER OXIDASES THAT HAVE STRONG OXIDATION ABILITIES TOWARDS PHENOLIC COMPOUNDS. INTERESTINGLY, NEW ISOLATED LACCASE SHOWED THE REMARKABLE BEHAVIOR OF ACTIVATION AFTER THERMAL TREATMENT. THE ACTIVITY INCREASED AT LEAST 300% ALSO THE KCAT WAS SUCCESSFULLY ENHANCED (2.5 FOLD) AFTER 50 MIN INCUBATION AT 70OC. DUE TO THE LIMITED KNOWLEDGE PRESENTED ON THIS INTERESTING PHENOMENON, THE PRESENT PAPER CONCERNS THE DIFFERENCES WHICH ARE INDUCED IN ENZYME STRUCTURE AFTER THERMAL TREATMENT.THE LOCAL CHANGES IN SECONDARY STRUCTURES WERE ALSO OBSEVED. OLIGOMERIZATION STUDIES OF THE ENZYME USING GAS-PHASE ELECTROPHORETIC MOBILITY MACROMOLECULE ANALYSIS (GEMMA) DID NOT PROVE ANY OLIGOMERIZATION.

INTRODUCTION: GENE DELIVERY TO STEM CELLS HOLDS GREAT POTENTIAL FOR TISSUE REGENERATION AND DELIVERY OF THERAPEUTIC PROTEINS. THE MAJOR BARRIER IS THE LACK OF SAFE AND EFFICIENT DELIVERY METHODS. NON-VIRAL VECTORS BASED ON THE USE OF CATIONIC POLYMERS APPEAR TO HAVE PROMISING POTENTIAL, GIVEN THE PROBLEMS OF SAFETY ENCOUNTERED WITH VIRAL VECTORS...

THIS STUDY ESTABLISHED THE IN VITRO CULTURE OF CATHARANTHUS ROSEUS HAIRY ROOTS USING RI PLASMID [PRI] T-DNA OF ATCC15834 STRAIN OFAGROBACTERIUM RHIZOGENES AND THEN PRODUCTION OF ARTIFICIAL SEEDS WITH HAIRY ROOT INOCULUMS AS PROMISING VEHICLE FOR PROPAGATION AND CONSERVATION OF THIS VALUABLE HAIRY ROOT OF C.

PROOXIDANT-ANTIOXIDANT BALANCE (PAB) IN VARIATION CONCENTRATION EXTRACTS OFTHUJA ORIENTALIS L. BY AMODIFICATION PAB ASSAY AND ANTIOXIDANT CAPACITY OF THIS PLANT WAS EVALUATED. THE ASSAY IS BASED ON 3, 3′, 5, 5′-TETRAMETHYLBENZIDINE AND ITS CATION, USED AS A REDOX INDICATOR PARTICIPATING IN TWO SIMULTANEOUS REACTIONS.PAB WAS DETERMINED IN DIFFERENT SOLVENT EXTRACT IN COMPARISON TO VITAMIN C. THE PAB VALUE OF EXTRACT DECREASED WITH INCREASE IN EXTRACT CONCENTRATION. HIGHEST AMOUNT OF TOTAL PHENOLIC COMPOUND WAS FOUND IN METHANOL EXTRACT WHEREAS THE ETHYLACETATE EXTRACT CONTAINED HIGHEST AMOUNT FLAVONOIDS COMPARED TO OTHER EXTRACTS. THIS STUDY DEMONSTRATE THAT T.ORIENTALIS EXHIBIT ANTIOXIDANT ACTIVITY.

WE STUDIES VARIATION IN THE SIZES OF ERYTHROCYTE IN TWO SIMILAR SIZE AND CLOSELY RELATED SPECIES OF BATS (PIPISTRELLUS KUHLII AND HYPSUGO SAVII) LIVING IN WESTERN IRAN. THE LARGER ERYTHROCYTES WERE FOUND IN PIPISTRELLUS KUHLIIAND THE SMALLER IN HYPSUGO SAVII. THE RBC IN BOTH SPECIES SHOWS ACANTHOCYTOSIS, POIKILOCYTOSIS AND ANISOCYTOSIS. INPIPISTRELLUS KUHLII MOST OF THE RBC'S SHOW ACANTHOCYTOSIS WHILE IN HYPSUGO SAVII ONLY 50% OF RBC'S SHOW THESE CASES. INPIPISTRELLUS KUHLII 20% OF RBC’S ARE HYPOCHROMIC.

RECENT INTENSIVE FIELD WORK OF PSEUDOSCORPIONS FAUNISTIC IN KERMAN PROVINCE (2010-11) YIELDED INTERESTING RESULTS 17 GENERA BELONG NINE FAMILIES. TWO SPECIES, DACTYLOCHELIFER BRACHIALIS BEIER 1952 ANDCHTHONIUS EPHIPIOCHTHONIUS ANTOLICUS BEIER 1969, ARE REPORTED FOR FIRST IN KERMAN PROVINCE.D. BRACHIALIS HAS SEPARATED FROM DRY LEAF LITTERS BY SIEVING SAMPLES THAT COLLECTED FROM BIDKHAN, DALFARD, KHABR AND RAYEN. ANOTHER SPECIES HAS SEPARATED FROM MOISTURE SOILS BY BERLESE FUNNEL THAT COLLECTED FROM SE-KONJ.

CONVENTIONAL HYBRIDIZATION IN ROSELLE (HIBISCUS SABDARIFFA L.) IS DIFFICULT DUE TO ITS CLEISTOGAMOUS NATURE OF REPRODUCTION. TO OVERCOME THIS LIMITATION, AN INTENSIVE MUTATION BREEDING PROGRAM USING DIFFERENT DOSES OF GAMMA RADIATION WAS CONDUCTED. IN TOTAL, FIVE GENERATION OF THREE MUTANTS WERE DEVELOPED FROM THE VARIETY ‘ARAB’. A NUMBER OF MORPHO-AGRONOMIC AND PHYSICO-CHEMICAL CHARACTERISTICS INCLUDING PLANT HEIGHT, CANOPY DIAMETER, NUMBER OF BRANCHES PER PLANT, NUMBER OF FRUITS PER BRANCH, NUMBER OF FRUITS PER PLANT, WEIGHT OF FRUITS PER PLANT, WEIGHT OF FRESH CALYX PER PLANT, WEIGHT OF DRY CALYX PER PLANT, WEIGHT OF CAPSULE PER PLANT OF THESE MUTANTS OF ROSELLE (HIBISCUS SABDARIFFA L.), DESIGNATED UKMR-1, UKMR-2 AND UKMR-3, WERE EVALUATED IN TROPICAL ENVIRONMENT OF MALAYSIA. SIGNIFICANT DIFFERENCES WERE OBSERVED IN MORPHO-AGRONOMIC CHARACTERS IN THESE THREE MUTANTS. UKMR-2 SHOWED BETTER PERFORMANCE FOR WEIGHT OF FRUITS PER PLANT (2170.7 G) AND WEIGHT OF FRESH CALYX PER PLANT (1408.4 G); WHEREAS FOR NUMBER OF FRUITS PER BRANCH, NUMBER OF FRUITS PER PLANT AND WEIGHT OF CAPSULE PER PLANT UKMR-3 SHOWED BETTER PERFORMANCE. THE LIFE CYCLE OF MUTANTS WAS SHORTER AND THEIR LODGING RESISTANT WAS SIGNIFICANTLY HIGHER COMPARED TO THEIR PARENTS AND LOCAL CHECK.

BIOTECHNOLOGY HAS MADE HIGH CONTRIBUTIONS TO HEALTH CARE AND AGRICULTURE AND NOW SOCIETY IS BEGINNING TO USE THE BENEFITS OF BIOTECHNOLOGY'S "THIRD WAVE"-INDUSTRIAL AND ENVIRONMENTAL BIOTECH...

MANY SPECIES OF MEDICINAL PLANTS ARE USED IN THE TREATMENT OF ILLNESS. IN THIS STUDY IT WAS EVALUATED THE ANTICANCER ACTIVITY OF METHANOLIC EXTRACT OFFERULA ASSA-FOETIDA...

THE PRESENCE OF ACTIVE BIOLOGICAL COMPOUNDS IS DOCUMENTED IN DIFFERENT PLANTS INCLUDINGFERULA.DIVERSIN, A NATURAL PRENYL COUMARIN COMPOUND EXTRACTED FROMFERULA SPECIES, EXHIBITED SIGNIFICANTCYTOTOXIC EFFECTS ON 5637 CELLS, A TCC (BLADDER CARCINOMA) SUB-LINE, IN OUR PREVIOUS STUDIES AS REVEALED BY MTT ASSAY.

INTRODUCTION: IT SEEMS THAT A-CAMKII GENE WHICH IS HIGHLY EXPRESSED IN THE HIPPOCAMPUS, HAS AN IMPORTANT ROLE IN MEMORY FORMATION. IT HAS BEEN SHOWN THAT MORPHINE SENSITIZATION, CAN IMPROVE MEMORY PROCESSES VIA MOLECULAR MECHANISMS IN THE HIPPOCAMPUS. IN THIS STUDY, IN ORDER TO INVESTIGATE THE EFFECT OF MORPHINE SENSITIZATION ON A-CAMKII GENE EXPRESSION, WE USED REAL-TIME RT-PCR TO QUANTIFY THE EXPRESSION OF THIS GENE IN RIGHT AND LEFT LOBE OF DORSAL HIPPOCAMPUS IN NORMAL AND MORPHINE SENSITIZED RATS.

INTRODUCTION: CHEMOTHERAPY IS ONE OF THE MAIN CURING METHODS TO CANCER. DIFFERENT NATURAL AND SYNTHETIC COMPOUNDS ARE USED IN THIS FIELD; AMONG THEM ARE PLANTS COMPOUNDS THAT HAVE BEEN USED FOR A LONG TIME IN CANCER THERAPY...

OBJECTIVE: NANOFIBER SCAFFOLDS HAVE DEMONSTRATED THEIR POTENTIALS AS MEDICAL IMPLANT BIOMATERIALS. NEURAL STEM CELLS (NSCS) GROWN ON NANOFIBER SCAFFOLD MAY BE USEFUL FOR REPAIRING NERVOUS SYSTEM INJURY. THE AIM OF THIS STUDY WAS TO PRODUCE NEURAL STEM CELLS FROM HUMAN INDUCED PLURIPOTENT STEM (HIPS) CELLS AND ASSAY THEIR PROLIFERATION ON PCL SCAFFOLD IN VITRO….

IN THIS WORK THE PREVALENCE OFAEROMONAS HYDROPHILA, A PATHOGENIC BACTERIUM FOR AQUACULTURE, WAS EVALUATED FROM MARKET FISH MEAT SAMPLES IN KERMAN CITY.240 SAMPLES WERE COLLECTED AND CULTURED IN PROMOTING PEPTONE WATER CULTURE AND THEN IN SELECTED MEDIUM GLUTAMATE STARCH PHENOL AGAR (GSPA) RESPECTIVELY. YELLOW COLONIES WHICH REPRESENTA. HYDROPHILA GROUPS WERE SELECTED AND FOLLOWED FOR ANALYSIS.RESULTS SHOWED THAT AROUND 24% OF FISH MEAT SAMPLES WERE INFECTED BYA. HYDROPHILA. SINCE THESE STRAINS OF BACTERIA ARE VERY DIVERSE, MORE PRECISE TECHNIQUES AND CULTURES SHOULD BE USED FOR ACCURATE ISOLATION AND IDENTIFICATION.

INTRODUCTION: EPILEPSY IS A CHIEF COMMUNAL HEALTH PROBLEM. EXISTING ANTIEPILEPTIC DRUGS ONLY PROVIDE SYMPTOMATIC TREATMENT AND THERE IS A GREAT NEED FOR THE DEVELOPMENT OF ALTERNATIVE THERAPEUTIC APPROACHES FOR PREVENTION AND TREATMENT OF EPILEPTIC SEIZURES. WALNUT KERNELS (WK) HAVE HIGH CONCENTRATIONS OF PHENOLIC COMPOUNDS, WHICH HAVE BENEFICIAL EFFECTS ON HUMAN HEALTH BECAUSE OF THEIR ANTIOXIDANT AND ANTI-ATHEROGENIC PROPERTIES. THE PRESENT STUDY WAS DESIGNED TO EVALUATE THE EFFECT OF WK EXTRACT ADMINISTRATION ON THRESHOLD AND SEVERITY OF EXPERIMENTAL CONVULSION IN RAT...

A RECOMBINANT FORM OF LACCASE (BENZENEDIOL OXYGEN OXIDOREDUCTASES, EC 1.10.3.2) FROM BACILLUS SP.HR03 WAS OVEREXPRESSED IN ESCHERICHIA COLI BL-21 (DE3). INCLUSION BODY FORMATION HAPPENS QUITE OFTEN DURING RECOMBINANT PROTEIN PRODUCTION. REFOLDING CONDITIONS TO YIELD ACTIVE ENZYMES-USING DILUTION METHOD -WERE OPTIMIZED TAKING AS A STARTING POINT FOR STUDYING BACTERIAL LACCASE. SOLUBILIZATION OF INCLUSION BODIES WAS CARRIED OUT IN PHOSPHATE BUFFER PH 7, CONTAINING 8 M UREA AND 4 MMB-MERCAPTOETHANOL. THE EFFECT OF DIFFERENT ADDITIVES WAS INVESTIGATED ON THE REFOLDING PROCEDURE OF DENATURATED LACCASE. ALSO KINETIC PARAMETERS OF SOLUBLE AND REFOLDED LACCASE WERE ANALYZED.

IN PRESENT STUDY, LENGTH-WEIGHT RELATIONSHIPS ARE DESCRIBED FOR 2 IMPORTANT FISH SPECIES THAT ARE NATIVE AND ECONOMIC IN THE SISTAN BASIN (SOUTH EASTERN OF IRAN): SNOW TROUT (SCHIZOTHORAX ZARUDNYI) AND SNOW TROUT (SCHIZOCYPRIS ALTIDORSALIS). SAMPLES WERE COLLECTED WITH GILL NETS FROM CHAHNIMEH RESERVOIRS.THE SAMPLE SIZE RANGED FROM 134 INDIVIDUALS FOR SCHIZOTHORAX ZARUDNYI TO 204 FOR SCHIZOCYPRIS ALTIDORSALIS. LINEAR REGRESSION EQUATIONS WERE OBTAINED. THE DATASET HAS SHOWN APPROPRIATENESS WHICH FOLLOWS ALLOMETRIC GROWTH.

IN THIS STUDY A PARASITOLOGICAL ONCAPOETA DAMASCINA FROM OCTOBER 2007 TO SEPTEMBER 2008. TOTALLY 109 FISHES HAVE BEEN SEINED FROM FOUR STATION INCLUDING: 1) HALIL 2) KONAROIYEH 3) AB SHUR 4) JAFAR ABAD. SAMPELS WERE SEINED BY ELECTRO SHOKER...

CELL DEATH OCCURS VIA DIFFERENT MECHANISMS INCLUDING APOPTOSIS AND NECROSIS IN MAMMALIAN CELLS, FOLLOWING EXPOSURE TO VARIOUS CHEMICAL AND PHYSICAL INSULTS. SEVERAL COUMARIN COMPOUNDS HAVE BEEN INTRODUCED WITH APOPTOSIS INDUCING PROPERTY IN RECENT YEARS.

INTRODUCTION AND OBJECTIVE: HELICOBACTER PYLORI IS A WIDELY DISTRIBUTED GRAM NEGATIVE BACTERIUM THAT INFECTS THE HUMAN STOMACH AND DUODENUM.HPAA IS A HELICOBACTER PYLORI SPECIFIC LIPOPROTEIN THAT HAS BEEN SHOWN TO BE AN EFFECTIVE PROTECTIVE ANTIGEN FOR MUCOSAL VACCINATION AGAINSTH. PYLORI INFECTION IN MICE. HPAA OF HELICOBACTER PYLORI AS A VACCINE ANTIGEN IS FULLY COMPETENT FOR STIMULATION OF IMMUNE RESPONSES AND HERE WE REPORT THE EXPRESSION OF THE PROTEIN IN A RECOMBINANT FORM...

THE SOLVENT DIELECTRIC CONSTANT IS CONSIDERED AN IMPORTANT FACTOR IN DETERMINING THE PROPERTIES OF THE HEME-CONTAINING PROTEIN CYTOCHROME C IN SOLUTION. THEREFORE, WE INVESTIGATE SOLVENTS EFFECTS ON THE RELATIVE ENERGIES, DIPOLE MOMENT VALUES, AND STRUCTURAL PROPERTIES OF WATER, METHANOL, AND ETHANOL SURROUNDING HEME C (HEC), BY USING MOLECULAR DYNAMICS SIMULATION. IN THIS STUDY, WE SUBSTITUTE THE IRON ATOM OF HEC WITH OTHER METAL ATOMS SUCH AS COBALT, NICKEL, COPPER AND ZINC AND POLAR SOLVENT EFFECT HAS BEEN PERFORMED USING ONSAGER MODEL. CALCULATIONS WERE CARRIED OUT WITH THE GAUSSIAN 98 PROGRAM BASED ON DENSITY FUNCTIONAL THEORY (DFT) AT B3LYP/6-31G* LEVEL. WE HAVE COMPARED GAS PHASE AND SOLVENT CALCULATIONS.

BACKGROUND AND PURPOSE: KLEBSIELLA PNEUMONIAEAND STAPHYLOCOCCUS AUREUS (MDR) ARE OF MAJOR HOSPITAL PATHOGENS THAT ARE THEPOTENTIAL CAUSES OF SEVERE INFECTIONS, ESPECIALLY IN THE INTENSIVE CARE UNIT AND IS AMONG THE MANY PATIENTS, ON THE OTHER HAND NANOPARTICLES OF TITANIUM DIOXIDE HAS A VERY STRONG ANTIBACTERIAL ACTIVITY AGAINST MOST BACTERIA.THE MAIN OBJECTIVE OF THIS RESEARCH IS STUDY THE EFFECTS OF TIO2 NANOPARTICLES ON THE BACTERIA RESISTANT TO MULTIPLE ANTIBIOTICS (MDR) IS LISTED.

IN PRESENT STUDY, LENGTH-WEIGHT RELATIONSHIPS ARE DESCRIBED FOR 2 IMPORTANT FISH SPECIES THAT ARE NATIVE AND ECONOMIC IN THE SISTAN BASIN (SOUTH EASTERN OF IRAN): SNOW TROUT (SCHIZOTHORAX ZARUDNYI) AND SNOW TROUT (SCHIZOCYPRIS ALTIDORSALIS). SAMPLES WERE COLLECTED WITH GILL NETS FROM CHAHNIMEH RESERVOIRS.THE SAMPLE SIZE RANGED FROM 134 INDIVIDUALS FORSCHIZOTHORAX ZARUDNYI TO 204 FOR SCHIZOCYPRIS ALTIDORSALIS. LINEAR REGRESSION EQUATIONS WERE OBTAINED. THE DATASET HAS SHOWN APPROPRIATENESS WHICH FOLLOWS ALLOMETRIC GROWTH.

BACK GROUND-GENOME WIDE SNP ASSOCIATION STUDY (GWAS) HAS REPORTED SEVERAL SNPS THAT HAD POTENTIAL RELEVANCE TO OLIGOSPERMIA AND AZOOSPERMIA IN IDIOPATHIC INFERTILE MEN IN EUROPEAN POPULATION. FOUR OF THESE RELEAVANT SNPS ARE IN INSR (T132903C), SLC6A14 (C109869T), OR2W3 (T824C) AND TAS2R38 (T886C) GENES HAVE REPORTED TO CAUSE OLIGOSPERMIA AND AZOOSPERMIA IN EUROPEAN POPULATIONS.

PESTICIDES HAVE BEEN EXTENSIVELY APPLIED IN RECENT DECADES IN AGRICULTUREL THROUGHOUT THE WORLD. THEY ARE POWERFUL TOXICANTS WHICH ARE EASILY ABSORBED AND TEND TO ACCUMULATE ONTO THE SOIL, PLANTS, FOODS, GROUND AND SURFACE WATERS. THE IN VITRO GENOTOXICITY STUDY OF ORGANOCHLORO HERBICIDES IS VERY LACK. SO THE OBJECTIVE OF THE PRESENT STUDY WAS TO EVALUATE THE GENOTOXIC EFFECTS OF THE CLODINAFOP-PROPARGYL (CP), WHICH IS WIDELY USED IN PEST-CONTROL PROGRAMS IN AGRICULTURE AND IN PUBLIC HEALTH AS WELL.CP CLASSIFIED AS "LIKELY TO BE CARCINOGENIC TO HUMANS" BY THE ORAL ROUTE BASED ON THE OCCURRENCE OF PROSTATE TUMORS IN MALE RATS, OVARIAN TUMORS IN FEMALE RATS, AND LIVER TUMORS IN BOTH SEXES OF MICE, AS WELL AS BLOOD VESSEL TUMORS IN FEMALE MICE.1 THIS COMPOUND IS HYDROLYZED WITHIN PLANT TISSUES TO RELEASE THE PHYTOTOXIC ACID OR ALCOHOL.2 IT ALSO TARGETS THE FATTY ACID BIOSYNTHETIC PATHWAY OF GRASSES BY INHIBITING THE PLASTID FORM OF THE ENZYME ACETYL- COA CARBOXYLASE (ACCASE; EC 6.4.1.2).

FEEDING HABIT IN THREE POPULATIONS OF FIRE SALAMANDER, SALAMANDRA INFRAIMMACULATA SEMENOVIHAVE BEEN STUDIED AT ZAGROS MOUNTAIN IN WESTERN PART IF IRAN, KURDISTAN AND KERMANSHAH PROVINCES IN JUNE 2011. WE CONDUCTED STUDY USING ADUTS.I.SEMENOVI TO DETERMINE (1) THE PREY COMPOSITION OF ADULT SALAMANDER, (2) PROBABILITY DIFFERENCES BETWEEN FOOD HABITS OF THIS SPECIES IN THREE POPULATIONS, (3) WHETHER ADULT SIZE AFFECTS THE DIVERSITY OF PREY ITEMS AND (4) WHETHER NONLETHAL STOMACH FLUSHING IS AN EFFECTIVE TECHNIQUE FOR EXAMINING THE DIET OFS.I.SEMENOVI, AND IS THERE A SIGNIFICANCE DIFFERENCES IN RESULTS OF PREY ITEM EXAMINATION BETWEEN NONLETHAL TECHNIQUE (STOMACH – FLUSHING) AND DIRECT OBSERVATION (DISSECTION).S.I.SEMENOVIS ADULTS CONSUMED A WIDE DIVERSITY OF PREY ITEMS INCLUDING SMALL TERRESTRIAL INVERTEBRATES SPECIALLY INSECTS, THE FEEDING HABITS OBTAINING DATA SHOWS THAT COLEOPTERAN WITH 38.3% OF PREY ITEMS HAVE THE MOST FREQUENCY IN FOOD CONTENTS. GASTROPODA WITH 31.7% HAVE THE SECOND PLACE IN THE PREY ITEMS2FREQUENCIES. HYMENOPTERA WITH 3.3% OF PREY ITEM HAVE THE LEAST FREQUENCY IN S.I.SEMENOVIFOOD CONTENT. WE FOUND SOME DIFFERENCES IN PREY ITEMS FREQUENCY BETWEEN THREE POPULATIONS. THE RESULTS SHOW THAT THE LARGER SALAMANDER (DISTINGUISHED WITH S.V.L CHARACTER, SNOUT – VENT LENGTH TO THE POSTERIOR TIP OF VENT) CONSUMED A WIDER DIVERSITY OF PREY ITEMS THAN SMALLER INDIVIDUALS. OUR RESULT ALSO SUGGESTED THAT NONLETHAL STOMACH FLUSHING DID NOT AFFECT SURVIVORSHIP AND ALSO THERE IS NO SIGNIFICANCE DIFFERENCE IN RESULT OF PREY ITEMS EXAMINATION USING THE STOMACH FLUSHING AND DISSECTION, SO USING THE STOMACH FLUSHING FOR FEEDING HABIT EXAMINATION ACCORDING TO THE NUMBER LIMITATION AND IUCN (2010) THAT CATEGORIZED S.I.SEMENOVIAS NEAR THREATENED IS ESSENTIAL.THESE FINDING SUGGEST THAT S.I.SEMANOVI IS A GENERALIST PREDATOR THAT PLAY IMPORTANT TROPHIC ROLES IN TERRESTRIAL ECOSYSTEMS.

MICRORNA IS AN ATTRACTIVE TOOL FOR FUTURE THERAPIES WHICH REGULATES GENE EXPRESSION BY BINDING TO TARGET MRNA AND DEPENDING ON THE DEGREE OF COMPLEMENTARY BINDING, REGULATES GENE EXPRESSION BY EITHER REPRESSION OF TRANSLATION OR DEGRADATION OF MRNA...

HUMAN CARBONIC ANHYDRASE II (HCA II, EC 4.2.1.1) IS A METALLOENZYME WHICH CATALYZES THE REVERSIBLE HYDRATION OF CARBON DIOXIDE TO BICARBONATE AND PROTON. IN THIS STUDY, WE REPORTED THE EFFECT OF TOPIRAMATE...

IONIZING RADIATION CAN RESULT IN REPRODUCTIVE DEATH BY INDUCING SINGLE STRAND AND DOUBLE STRAND DNA BREAK IN MALIGNANT CELLS. IN ORDER TO IMPROVE THE EFFICACY OF RADIOTHERAPY...

INFERTILITY IS A UNIQUE MEDICAL CONDITION BECAUSE IT INVOLVES A COUPLE RATHER THAN A SINGLE INDIVISUAL AND IT IS DEFINED AS AN INABILITY TO CONCEIVE AFTER ONE YEAR OF UNPROTECTED INTERCOURSE. MALE FACTOR IS SOLELY RESPONSIBLE NEARLY 50%...

CELL EXPOSE TO DRUGS HAS BEEN NECESSARILY EVALUATED BY IN VITRO LAB SETS TAKEN HIGH COST, BUT, USE OF SINGLE- CELLED ANIMALS IS UNCOMMON. IN THIS STUDY PARAMECIUM CAUDATUM WAS PRESENTED THE NARCOTIC DRUG NALOXONE. THE ANIMAL UP TO 600 MM WAS COLLECTED FROM NATURAL SOURCES AND AFTER SPECIFIC DETERMINATION ABUNDANTLY CULTIVATED IN SPECIFIC MEDIUM. A VOLUME1ML OF THE MEDIUM CONTAINING THE ORGANISMS WAS ADDED INTO THE SEDGWICK- RAFTER CELL COUNTER AND 1ML OF DRUG WAS INFUSED INTO THE COUNTER…..

THE AIM OF THIS STUDY WAS TO STUDY TOXICITY OF ORGANOPHOSPHATE (OP) PESTICIDES IN EXPOSED FARMERS IN TERMS DIABETES AND HEMATOLOGICAL FACTORS. A COMPARATIVE CROSS-SECTIONAL ANALYSIS WAS DONE IN 174 FARMERS WHO HAD BEEN EXPOSED TO OPS IN COMPARISON TO A CONTROL GROUP CONTAINING 174 HEALTHY SUBJECTS WITH THE SAME AGE AND SEX AND EDUCATION LEVEL. THE LEVELS OF FASTING BLOOD GLUCOSE, BLOOD UREA NITROGEN (BUN), CHOLESTEROL, TRIGLYCERIDES, CREATINIINE, HIGH-DENSITY LIPOPROTEIN (HDL), ASPARTATE AMINOTRANSFERASE (AST) AND ALANINE….

SECONDARY METABOLITES ARE ORGANIC COMPOUNDS THAT ARE NOT DIRECTLY INVOLVED IN THE NORMAL GROWTH, DEVELOPMENT, OR REPRODUCTION OF AN ORGANISM. SEPARATION AND QUANTITATIVE DETERMINATION OF A WIDE VARIETY OF ORGANIC AND INORGANIC COMPONENTS IN MICROORGANISMS ARE CARRIED OUT BY CHROMATOGRAPHIC METHODS. IN THIS STUDY, ASPERGILLUS OSTIANUSSPORES WAS GROWN IN A LIQUID CULTURE OF POTATO DEXTROSE BROTH (PDB) AND THE BROTH WAS FILTERED. THE FILTRATE WAS EXTRACTED WITH ETOAC. THE PRODUCTION OF COMPOUNDS WERE DETERMINED BY TLC AND GC-MS. THE RESULTS SHOWED THAT, A.OSTIANUS PRODUCED DIFFERENT COMPOUND SUCH AS: OCTANOIC ACID, METFORMIN, LINOLEIC ACID, OLEIC ACID, PALMITIC ACID, MYRISTIC ACID AND SQUALENE.

GENERATION OF MULTIPLE ANTIBIOTIC RESISTANT PHENOTYPE (RESISTANT TO CIPROFLOXACIN AND TETRACYCLINE) INE. COLICAN BE RELATED TO CHANGE IN PERMEABILITY OF CELL MEMBRANE THAT INHIBITS THE ENTRANCE OF ANTIBIOTICS FROM OUTSIDE TO INSIDE.

THYROID HORMONES MAY BE CONSIDERED AS A BIOMARKER DUE TO THEIR IMPORTANCE IN METABOLISM, HOMEOSTASIS, AND HIGH SENSITIVITY TO POLLUTANTS. THE PRESENT RESEARCH WAS CONDUCTED TO STUDY THE EFFECT OF BENZO ALPHA PYRENE (BAP) ON THE PLASMA LEVELS OF THYROXIN (T4), TRIIODOTHYRONINE (T3) HORMONES, AND T3/T4 RATIO IN YELLOWFIN SEABREAM, ACANTHOPAGRUS LATUS. THEREFORE, PERITONEAL INJECTION OF BAP DISSOLVED IN VEGETABLE OIL (50MG/KG BODY WEIGHT IN 2ML/G OIL ACCORDING TO THEIR BODY WEIGHT) WAS PERFORMED ON A GROUP OF FISH. THE CONTROL GROUP RECEIVED ONLY 2ML/G VEGETABLE OIL BASED UPON THEIR BODY WEIGHT. BLOOD SAMPLE WAS TAKEN FROM BOTH GROUPS AFTER THREE HOURS…..

SAMBUCUS NIGRAL-AGGLUTININ-I REFERRED TO AS SNA-I IS A CHIMERIC LECTIN COMPOSED OF AN A-CHAIN WITH ENZYMATIC ACTIVITY AND A B-CHAIN WITH CARBOHYDRATE-BIDING ACTIVITY. BCIRL-LEPD-SL1 (CPB) CELL LINE ESTABLISHED FROM PUPA FAT BODY OF THE COLORADO POTATO BEETLE, LEPTINOTARSA DECEMLINEATA (COL.CHRYSOMELIDAE). TREATMENT OF INSECT PUPAL FAT BODY CELLS WITH SNA-I CAUSED A LOSS OF CELL VIABILITY AFTER FOUR DAYS OF INCUBATION WITH DIFFERENT CONCENTRATIONS OF SNA-I (0.1- 30 MG/ML) BASED ON THE MTT TEST. IT WAS CLEAR THAT SNA-I HAD A STRONG GROWTH INHIBITORY EFFECT ON CPB CELLS. INCREASED CYTOTOXICITY WAS OBSERVED IN SNA-I TREATED CELLS IN A DOSE AND TIME DEPENDENT MANNER.

ENDOMETRIOSIS CHARACTERIZED BY THE PRESENCE OF ECTOPIC ENDOMETRIAL TISSUE OUTSIDE THE UTERINE CAVITY. ENDOMETRIOSIS IS A MULTIFACTORIAL DISEASE...

LIGULARIA PERSICABOISS. IS AN IMPORTANT GENUS OF THE COMPOSITAE FAMILY (TRIBE SENECIONEAE). THE PLANT WAS COLLECTED DURING THE FLOWERING STAGE IN JULY 2011. WE PROVIDED ESSENTIAL OILS WITH CLEVENGER-TYPE APPARATUS FOR 2 H. THE PURPOSE OF THIS STUDY WAS TO DETERMINE THE ANTIBACTERIAL EFFECTS OF ESSENTIAL OILS OBTAINED FROM DIFFERENT PARTS OF L.PERSICA. ANTIBACTERIAL ACTIVITY WERE TESTED ON 2 GRAM-POSITIVE (STAPHYLOCOCCUS AUREUS AND STREPTOCOCCUS SUBRINOS) AND 2 GRAM-NEGATIVE BACTERIA (ESCHERICHIA COLI AND PSEUDOMONAS AERUGINOSA), USING MICRODILUTION METHOD. THE MINIMUM INHIBITION CONCENTRATION (MIC) OF THEL. PERSICA WAS 71.67ΜG/ΜL AGAINST PSEUDOMONAS AERUGINOSA. THE ESSENTIAL OILS FROML. PERSICA ROOTS SHOWED HIGHER INHIBITORY ACTIVITY AGAINST ALL OF THOSE BACTERIA.

GENERATION OF MULTIPLE ANTIBIOTIC RESISTANT PHENOTYPE (RESISTANT TO CIPROFLOXACIN AND TETRACYCLINE) INE.COLICAN BE RELATED TO INCREASE IN MEMBRANE TRANSPORTER ACTIVATION THAT HAS A ROLE IN EFFLUX OF ANTIBIOTICS FROM CELL.

TWO ADULT MALEHYPSUGO SAVII WERE MIST-NETTED AT RAZI UNIVERSITY CAMPUS IN KERMANSHAH, WESTERN IRAN, IN A LOCALITY NOT PREVIOUSLY KNOWN AS A HABITAT FOR THIS BAT. THEY SHOWED FUSED EPIPHYSAL GAP AND THE MALE CAUGHT IN OCTOBER HAD AN ENLARGED EPIDIDYMES INDICATING A FULFILLED SPERMATOGENESIS CYCLE. ON THE BASIS OF SIMILARITY FOUND BETWEEN PLEDGE COLORATION AND THE ABSENCE OF P3 UPPER PREMOLAR WE INFERRED THAT POPULATIONS OF HYPSIGO SAVII OCCURRING IN WESTERN IRAN RESEMBLES TO H. S. CAUCASICUS DESCRIBED BY OTHER AUTHORS. THIS IS THE FIFTH RECORD OF H. SAVII IN IRAN AND THE FIRST FOR MID-ZAGROS RANGE IN WESTERN IRAN.

SHIGELLOSIS IS A MAJOR PUBLIC HEALTH IN DEVELOPING COUNTRIES AND IS THE MAJOR ETIOLOGIC AGENT OF TRAVELER’S DIARRHEA. SHIGELLOSIS IS A GASTROINTESTINAL DISEASE CAUSED BYSHIGELLA SPP. THE INCREASING PROBLEM OF ANTIBIOTIC RESISTANCE ALERTS ON THE URGENT NEED OF PROTECTIVE VACCINES. RECENTLY, SEVERAL IMMUNOGENIC PROTEINS OFS. FLEXNERI 2A HAVE BEEN IDENTIFIED BY IMMUNOPROTEOMICS ANALYSIS. A PROTEIN WITH ACCESSION NO. NP_836134.1 WAS IDENTIFIED AS THE BEST PROTEIN USES VAXIJENE SOFTWARE.HENCE THE IDENTIFICATION OF ITS STRUCTURE IS VERY IMPORTANT. BIOINFORMATIC TOOLS SUGGEST THAT THIS PROTEIN IS COMPOSED OF 6 ALPHA HELIX AND 19 BETA STRAND.

MACROPHAGES PARTICIPATE IN MANY NORMAL PROCESSES SUCH AS APOPTOSIS, NECROSIS, HOMEOSTASIS, AND ADAPTIVE AND INNATE IMMUNITY; HOWEVER, WHEN OVER ACTIVATED OR DEFECTIVE, THEY CONTRIBUTE TO DISEASES, INCLUDING MACROPHAGE ACTIVATION SYNDROME (MAS), DIABETES, HUMAN IMMUNODEFICIENCY VIRUS (HIV) INFECTION, ATHEROSCLEROSIS, AND CANCER...

THE INTRACTION BETWEEN COLCHICINE (COL) AND HUMAN SERUM ALBUMIN (HSA) IN THE PRESENCE OF ASPIRIN (ASA) AS TWO NANOPARTICLE DRUGS IN PHYSIOLOGICAL CONDITION (PH=7.4) WAS INVESTIGATED BY FLUORESCENCE SPECTROSCOPY. THE CHANGE ON THE PROTEIN CONFORMATION UPON BINDING WAS FOLLOWED AS A FUNCTION OF ADDED DRUG BY THREE-DIMENSIONAL. CHANGE IN THE ENVIRONMENT OF THE AROMATIC RESIDUES ARE ALSO OBSORVED. THE RESULTS INDICATED THAT THE STRUCTURE OF TRP AND TYR RESIDUES ENVIRONMENTS WAS ALTERED AND PHYSIOLOSICAL FUNCTION OF DRUGS WERE AFFECTED BY HSA. IT WAS SHOWN THAT THE FLUORESCENCE OF HSA HAVE BEEN QUENCHED FOR REACTING WITH DRUGS AND FORMING A CERTAIN KIND OF NEW COMPOUND. TREE-DIMENSIONAL FLUORESCENCE SPECTROSCOPY SHOWS THAT THE SECONDARY STRUCTURE OF HSA CHANGED AFTER DRUGS BOUND TO HSA IN BINARY AND TERNARY SYSTEMS.

BIOTECHNOLOGY HAS MADE HIGH CONTRIBUTIONS TO HEALTH CARE AND AGRICULTURE AND NOW SOCIETY IS BEGINNING TO USE THE BENEFITS OF BIOTECHNOLOGY'S "THIRD WAVE"-INDUSTRIAL AND ENVIRONMENTAL BIOTECH.MODERN BIOTECHNOLOGY PROVIDES NEW WAYS FOR ACHIEVING THESE GOALS. WITHOUT BIOINFORMATICS, NEW RESEARCH IN MOST FIELDS OF MEDICINE AND BIOLOGY WOULD COME TO BE INCOMPLETE. THROUGH A COMBINATION OF BIOINFORMATICS AND GENETICS ENGINEERING PROCEDURES, WE HAVE DESIGNED A HIGH-AFFINITY METAL BINDING PEPTIDES AND EXPRESSED THEM ON THE SURFACE OFE.COLI FOR SEQUESTRATION AND SPECIFIC ADSORPTION OF CADMIUM FROM POLLUTED WATER...

MOLECULAR DOCKING TECHNIQUES HAVE BECOME ONE OF THE MOST POPULAR AND SUCCESSFUL APPROACHES IN DRUG DISCOVERY. THIS PAPER DESCRIBES THE COMPREHENSIVE EVALUATION DOCKING OF METHICILLIN CONTAINING ISOXAZOLE DERIVATIVES AS INHIBITORS TO STAPHYLOCOCCUS AUREUS.THE INHIBITORY ACTIVITIES AGAINST S. AUREUS B-KETOACYL-ACYL CARRIER PROTEIN (ACP) SYNTHESIS III (FABH) WERE ENQUIRED BY MOLECULAR DOCKING USING THE HEX DOCKING SOFTWARE (V 6.1). RESULTS CONFIRM ALL THE DESIGNED COMPOUNDS WERE GOOD BINDINGENERGY WHEN COMPARED WITH THE BINGING ENERGIES OF STANDARD DRUGS SUCH AS VANCOMYCIN (-179.66), CIPROFLOXACIN (-147.11) AND GENTAMCIN (-234.45). AMONG ALL THE DESIGNED COMPOUNDS, THE COMPOUND 4 INDICATE MORE BINDING ENERGY VALUE (-327.48). THE PRESENT STUDY THEREFORE WE DESIGNED TO SYNTHESIS THESE METHICILLIN DERIVATIVES AND SCREEN FOR IN-VITRO ANTIBACTERIAL EFFECT ON S. AUREUS AND OTHER MICROORGANISMS.

OBJECTIVE: MEDICINAL HISTORY OFCANNABIS SATIVA GOES BACK TO BC. THE PRESENT STUDY IS AIMED TO INVESTIGATE RESTORATIVE AND ANTI-INFLAMMATORY EFFECTS OF AQUATIC EXTRACT OF CANNABIS SATIVA LEAVES ON SPINAL GLIAL AND ALPHA-NEURONS FOLLOWING CENTRAL DEGENERATION IN RATS.METHODS: THIS EXPERIMENTAL STUDY WAS CONDUCTED ON 32 MALE WISTAR RATS. ANIMALS WERE DIVIDED INTO 4 GROUPS; A: CONTROL GROUP, B: COMPRESSION, C: COMPRESSION+TREATMENT WITH A DOSE OF 25 MG/KG, AND D: COMPRESSION+TREATMENT WITH A DOSE OF 50 MG/KG. AFTER SCIATIC NERVE COMPRESSION IN TREATMENT GROUPS B, C, AND D, EXTRACT INJECTION WAS DONE INTRA-PERITONEAL IN GROUPS C AND D WITHIN 2 WEEKS (ONCE A WEEK). 28 DAYS AFTER COMPRESSION, LUMBAR SPINAL CORD WAS SAMPLED AND AFTER TISSUE PROCESSING, CUTTING, COLOURING WITH TOLUIDINE BLUE AND PREPARING PHOTOS FROM SPINAL ANTERIOR HORN USING A PHOTOMICROSCOPE, NEURONAL AND GLIAL DENSITY OF EACH GROUP WAS CALCULATED BY DISSECTOR METHOD. FINALLY DATA WAS ANALYSED USING ANOVA AND TUKEY TESTS…..

CENTRAL AMYGDALA (CEA) HAS AN IMPORTANT ROLE IN EMOTIONAL AND AVOIDANCE LEARNING. CEA NICOTINIC ACETYLCHOLINE RECEPTORS (NACHRS) ARE INVOLVED IN LEARNING AND MEMORY PROCESSES.3, 4-METHYLENEDIOXYMETHAMPHETAMINE (MDMA; ECSTASY) IS A POPULAR DRUG OF ABUSE WHICHPRODUCES EUPHORIA ANDCOGNITIVE ALTERATIONS. CONSIDERING THAT MDMA INCREASES CENTRAL ACETYLCHOLINE RELEASE, THE PRESENT STUDY EXAMINEDTHE EFFECTS OF THE BLOCKADE OF CEA NACHRS ON INTERACTIVE EFFECTS OF MORPHINE, NICOTINE AND MDMA ON MEMORY RETRIEVAL USING ASTEP-THROUGH PASSIVE AVOIDANCE TASK IN MALE WISTAR RATS. ALL ANIMALS WERE BILATERALLY IMPLANTED WITH GUIDE CANNULAS INTO THE CEA REGIONS. ANIMALS WERE ALLOWED 1 WEEK TO RECOVER FROM SURGERY…..

BACKGROUND AND OBJECTIVES: MICROORGANISMS THAT ARE RESISTANT TO BOTH ANTIBIOTICS AND METALS HAVE BEEN ISOLATED FROM NOSOCOMIAL AND BURN WOUND INFECTION. MOST OF THE HOSPITAL INFECTIONS INCLUDINGE.COLI AND KLEBSIELLA THAT ARE RESISTANT TO HEAVY METALS HARBOR THE PLASMIDS WITH DIFFERENT MOLECULAR SIZES. THE AIM OF THIS STUDY IS TO EVALUATE PATTERN OF HEAVY METAL RESISTANCE, MIC VALUES OF HEAVY METALS AND PLASMID PROFILES OF THE RESISTANT STRAINS...

THE CENTRAL NUCLEUS OF THE AMYGDALA (CEA) IS A FOREBRAIN STRUCTURE WHICH IS IMPORTANT IN REGULATE OF EMOTION AND INGESTIVE BEHAVIORS. THERE IS EVIDENCE TO INDICATE THAT SOME CIRCUITS INVOLVED WITH FEELING AND FEEDING BEHAVIORS INCLUDING GLUTAMATERGIC AND GABAERGIC ELEMENTS. THE PRESENT STUDY EXAMINED WHETHER ADMINISTRATION OF EITHER NMDA GLUTAMATERGIC AGONIST NMA (0.25-0.75 MG) OR NMDA ANTAGONIST MK801 (0.25-1 MG) AS WELL AS GABAA RECEPTOR AGONIST MUSCIMOL (0.25-LMG) OR GABAA RECEPTOR ANTAGONIST BICUCULLINE (0.25-1MG) IN THE CEA ALTERED FOOD INTAKE UNDER DEPRIVATION AND INDUCED SOCIAL ISOLATION…..

SEM STUDIES ARE AN IMPORTANT PREREQUISITE TO OBTAIN FURTHER INSIGHT INTO THE FINE STRUCTURES OF TELEOST EGGS.THE SCANNING ELECTRON MICROSCOPE WAS USED TO INVESTIGATE THE ULTRASTRUCTURES OF THE RIPE EGGS (STAGE V) MEMBRANE SURFACE (UNFERTILIZED EGG), CAPOETA MANDICA FROM RUDBAL RIVER, PERSIAN GULF BASIN, FARS PROVINCE. FOR SEM STUDY, THE RIPE EGGS WERE REMOVED FROM OVARIES AND FIXED AND PRESERVED IN 10% FORMALIN. THE EGGS WERE THEN CLEANED WITH A FINE BRUSH AND FORCEPS, WASHED WITH DISTILLED WATER AND THEN DEHYDRATED IN A GRADED SERIES OF ETHANOL (30, 50, 70, 90, AND 100%). THE EGGS WERE MOUNTED ON ALUMINUM STUBS COATED WITH THIN LAYER OF GOLD, AND WERE OBSERVED AND PHOTOGRAPHED WITH A LECIA CAMBRIDGE SCANNING ELECTRON MICROSCOPE (SEM) AT VOLTAGE OF 20 KV….

THE AIM OF THIS STUDY WAS TO STUDY TOXICITY OF ORGANOPHOSPHATE (OP) PESTICIDES IN EXPOSED FARMERS IN TERMS OF NEUROCOGNITIVE IMPAIRMENT, MENTAL HEALTH STATUS, CLINICAL SYMPTOM, DIABETES AND HEMATOLOGICAL FACTOR. A COMPARATIVE CROSS-SECTIONAL ANALYSIS WAS DONE IN 174 FARMERS WHO HAVE BEEN EXPOSED OPS IN COMPARISON TO A CONTROL GROUP CONTAINING 174 HEALTHY SUBJECTS WITH THE SAME AGE AND SEX AND EDUCATION LEVEL.THE LEVELS OF FASTING BLOOD GLUCOSE, BLOOD UREA NITROGEN (BUN), CHOLESTEROL, TRIGLYCERIDES, CREATININE, HIGH-DENSITY LIPOPROTEIN (HDL), ASPARTATE AMINOTRANSFERASE (AST) AND ALANINE AMINOTRANSFERASE (ALT) ANDALKALINE PHOSPHATES (ALKP) WERE MEASURED IN THE BLOOD OF SUBJECTS.THE MEAN LEVEL OF FASTING BLOOD GLUCOSE, BUN, CHOLESTEROL AND ALT WAS SIGNIFICANTLY RAISED IN WORKERS WHILECREATININE, TRIGLYCERIDES AND ALKALIN PHOSPHATASE SIGNIFICANTLY DECREASED AS COMPARED WITH CONTROLS. THE MEAN LEVELS OF AST AND FBS2 IN THE WORKERS AND CONTROLS WERE NOT STATISTICALLY DIFFERENT. THE NEUROCOGNITIVE IMPAIRMENT AND PSYCHOLOGICAL DISORDERS WERE SIGNIFICANTLY RAISED IN WORKERS..CLINICAL EXAMINATION WAS DONE IN ORDER TO RECORD ANY ABNORMAL SIGN OR SYMPTOMS. FARMERS SHOWED CLINICAL SYMPTOMS SUCH ASECZEMA, SALIVA SECRETION, FATIGUE, HEADACHE, SWEATING, ABDOMINAL PIAN,, NAUSEA, DISFOGHANI, DISTAHTANI, PROTAHTANI, AZOLATE SHEKAMI, HAND TINGLING, FOOT TINGLING, EPIPHORIA, EDRAR, MYOSIS, DYISPNEA, BRADYCARDIA, NASAL FLOWING. THE PRESENT FINDINGS INDICATE THAT INHIBITION OF ACHE CAN BE INCIDENCE OF NEUROPSYCHOLOGICAL DISORDERS AND PRE DIABETES. USE OF SUPPLEMENTARY ANTIOXIDANTS WOULD BE USEFUL IN THE TREATMENT OF FARMERS.

ICARIIN WAS TESTED ON LARVAL DEVELOPMENT AND METAMORPHOSIS OFAMPHIBALANUS AMPHITRITEAT DIFFERENT CONCENTRATIONS (10-7, 10-8, 10-9 MOLAR) ON BOTH NAUPLIUS AND CYPRIS LARVAL STAGES AND OBTAINED RESULTS OF COMPARISON THE CONTROL AND TESTS CONDITIONS HAD SHOWED THAT ICARIIN ACT AS PYRIPROXIFEN AN JUVENILE HORMONE BY AFFECTING DEVELOPMENT OF CYPRID LARVAE.

TEMPERATURE, AS WELL AS MANY OTHER ENVIRONMENTAL FACTORS AFFECTS RESPIRATION OF AQUATIC ORGANISMS INCLUDING AMPHIPOD CRUSTACEANS. THIS RESEARCH WAS CONDUCTED TO EVALUATE INTER-SPECIFIC VARIATION AMONG 3 SPECIES OF GAMMARUSIN DIFFERENT CLIMATIC CONDITIONS IN FARS PROVINCE...

THE RESEARCHERS ARE CONTINUOUSLY LOOKING FOR MORE SUITABLE MEDIA AND EFFECTIVE FACTORS TO IMPROVE IN VITRO FERTILIZATION. THE AIM OF THIS STUDY IS TO ASSESS THE EFFECT OF CAFFEINE ON SPERM'S MOTILITY, VITALITY AND LABORATORY FERTILIZATION RATES IN MOUSE IN TWO T6 AND M16 MEDIA. PRIOR TO IN VITRO FERTILIZATION, EPIDIDYMAL MOUSE SPERMS WERE TREATED BY CAFFEINE IN T6 AND M16 MEDIA AND THEIR MOTILITY AND VITALITY RATES WERE EVALUATED. THE PRETREATED SPERMS WERE ADDED TO OOCYTES IN T6 AND M16 MEDIA WITH AND WITHOUT CAFFEINE AND FERTILIZATION RATES WERE RECORDED AFTER 24 HOURS INCUBATION. RESULTS SHOW, SPERM'S MOTILITY AND VITALITY RATES IN T6 WITH CAFFEINE WERE 81.7±2.89% AND 88.7±2.31% AND WITHOUT CAFFEINE (CONTROL GROUP), 37.2±19.83% AND 47.9±14.46% RESPECTIVELY, AND IN M16 WITH CAFFEINE WERE 68.3±10.41% AND 78±10.58% AND IN CONTROL GROUP (M16 WITHOUT CAFFEINE) WERE 61.1±24.27% AND 77.4±12.06% RESPECTIVELY. PERCENTAGES OF FERTILIZATION (FERTILIZED OOCYTES) IN T6 WITH AND WITHOUT CAFFEINE WERE 67.52±16.33 AND 22.1±17.58, AND IN M16 WITH AND WITHOUT CAFFEINE WERE 42.6±25.91 AND 33.5±19.94 RESPECTIVELY. STATISTICAL ANALYSIS SHOW MOTILITY AND VITALITY OF SPERM AND PERCENTAGE OF FERTILIZATION IN T6 PLUS CAFFEINE COMPARE TO CONTROL GROUP (T6 WITHOUT CAFFEINE) WERE INCREASED AND HAVE SIGNIFICANT DIFFERENCES AT P≤0.05. BUT PERCENTAGE OF THESE PARAMETERS IN M16 PLUS CAFFEINE IN COMPARISON TO CONTROL GROUP (M16 WITHOUT CAFFEINE) DID NOT HAVE SIGNIFICANT DIFFERENCE. THESE RESULT SUGGEST, ADDITION OF CAFFEINE IN T6 MEDIUM IMPROVES SPERM'S MOTILITY AND VITALITY AND IN VITRO FERTILIZATION RATES.

LACTIC ACID BACTERIA ARE EMPLOYED IN DIFFERENT INDUSTRIES SUCH AS FOOD AND CHEMICAL INDUSTRIES.STREPTOCOCCUS THERMOPHILUSIS ONE OF THE TRADITIONAL STARTER CULTURES IN DAIRY INDUSTRIES. THIS STUDY WAS DESIGNED USING RESPONSE SURFACE METHODOLOGY IN 12 BATCH PH-CONTROLLED CULTURES OFSTREPTOCOCCUS THERMOPHILUSFOR DETERMINING THE EFFECT OF TEMPERATURE AND PH ON THE GROWTH AND ACID LACTIC PRODUCTION BY THIS STRAIN. RESPONSE SURFACE MODEL WAS SIGNIFICANT FOR MAXIMUM SPECIFIC GROWTH RATE OFS.THERMOPHILUS. MAXIMUM BIOMASS CONCENTRATION AND PRODUCED LACTIC ACID HIGHLY DEPENDED ON PH.OPTIMUM PH AND TEMPERATURE FOR THE GROWTH AND ACID PRODUCTION OFS.THERMOPHILUS WAS 42.8 AND 7, RESPECTIVELY.

CONSIDERING THE GLOBAL POPULARITY AND ALSO THE VARIOUS BIOLOGICAL AND MEDICINAL PROPERTIES OF SAFFRON, THIS STUDY WAS CONDUCTED TO ASSESS THE INFLUENCE OF ITS AQUEOUS EXTRACTS ADMINISTRATION ON BLOOD PRESSURE, PRESSURE-RATE PRODUCT (PRP) AND ELECTROCARDIOGRAM (ECG) INDICES OF RAT. ANIMALS WERE DIVIDED TO CONTROL (CTL), SAF50, SAF100, AND SAF200 GROUPS THAT ORALLY RECEIVED TAP WATER, AQUEOUS EXTRACTS OF SAFFRON 50, 100 AND 200 MG/KG/DAY RESPECTIVELY FOR SEVEN DAYS. ON DAY 8, DATA WERE RECORDED…..

AEROLYSIN GENE FROMA. HYDROPHILA WAS AMPLIFIED AND CLONED IN E. COLI. ELECTROPHORESIS, RE DIGESTION AND DNA SEQUENCING WERE PERFORMED TO VERIFY CLONING. RESULTS SHOWED A 1500 BPS THAT WAS IN AGREEMENT WITH THE PUBLISHED SIZES. IN ADDITION BLAST RESULTS SHOWED 95% SIMILARITY WITH REPORTED DATA. FURTHERMORE, RESTRICTION ENZYME EXPERIMENTS WITH SINGLE AND DOUBLE DIGESTIONS VERIFIED THE SUCCESSFUL CLONING. SINCE THE PCR AMPLIFICATION SHOWED A THICK BAND OF AEROLYSIN WE SUGGEST THAT PRIMERS IN THIS WORK ARE SUITABLE FOR AMPLIFICATION OF THE GENE OF INTEREST AND ALSO FOR ISOLATION OFA. HYDROPHILA FROM DIFFERENT SOURCES.