Background and Objective: In the 21st century, new perspectives emerged on the moral aspects of teachers in schools, and the role that virtue plays in fostering constructive emotions and interactions has highlighted the growing importance of virtue in improving psychological well-being. The aim of this study was to explain the mediating role of spiritual intelligence in the relationship between organizational virtue (as an Islamic religious variable) and psychological well-being among primary school teachers in Ardabil. Methods: The research method is descriptive correlational based on structural equation modeling. The statistical population of this study included all primary school teachers in Ardabil (no=3850), out of whom 350 were selected as the statistical sample of the research based on Cochran's formula and using multi-stage random cluster sampling method to collect data. Standard questionnaires of psychological well-being, organizational virtue, and spiritual intelligence were used to collect data. Also, structural equation modeling was used to analyze the data. In this study, all the ethical considerations were observed and the authors reported no conflict of interest. Results: The results of the correlation coefficient showed a significant correlation between the variables of organizational virtue, spiritual intelligence and psychological well-being in a positive direction. The results also showed that organizational virtue has a positive and significant direct effect on psychological well-being (0. 27). Organizational virtue has a positive and significant direct effect on spiritual intelligence (0. 84). Spiritual intelligence has a positive and significant effect on psychological well-being (0. 68). In addition, organizational virtue has a positive and significant indirect effect on psychological well-being (9. 74) through spiritual intelligence. Conclusion: The higher the teachers 'spiritual intelligence and vigilance in the organization, the higher the psychological well-being and job satisfaction. Therefore, virtue as an Islamic moral variable in the organization can affect the teachers' spiritual intelligence and psychological well-being.