Background and Aim: Wound healing is a complex process which is divided into four overlapping phases: heamostasis, inflammation, proliferation, and remodeling. In diabetic patients, progression of healing takes longer and thus using new medication to accelerate it is required. It is expected thatProsopis farcta which has antibacterial, antidiabetic and antiinflammation effects will facilitate diabetic wound healing. Thus, the present study aimed at investigation the effect of aqueous extract ofProsopis farcta root on wound healing in diabetic rats.Materials and Methods: In this experimental study, 18 wistar male rats were randomly divided into 3 equal groups: normal control, Diabetic- control, and experimental group (diabetes treatment with root extract). Diabetes was induced through intraperitoneally injection of Streptozocin (STZ). Then, in all groups, in two lateral posterial parts 3 holes (4 mm in diameter) were punched. For 2 days, 3 times on each, the experimental group was locally treated with root aqueous extract of Prosopis farcta. In the control groups normal saline was used in the same way. On the 4th, 8th and 10th day after punching samples were collected from the healing hole (which were now 6 mm in diameter). Histological observations revealed inflammatory cells, reepithelization, and neovascularization in the areas.Results: Significant changes in proliferation of inflammatory cells (on the 10th day), epithelium thickness (on the 4th and 8th day), more angiogenesis (on the 4th- 10th day) in the experimental wounds were compared with those in the diabetic control group (Respectively P<0.001, P<0.01).Conclusion: Considering the above findings, the extract of Prosopis farcta root, has effective roles on wound healing in diabetic rats, probably due to its anti- inflammatory, reepithelization, and neovascularization properties. However, more studies are needed in this respect.