Background & aim: Nursing jobs reduce psychological well-being and psychological capital due to stress. Life skills training as an educational method can improve the general health and quality of nurses’ life. The purpose of this study was to determine and study life skills training on psychological well-being and psychological capital in nurses of Shahid Beheshti Hospital in Yasuj. Methods: The present research was a semi-experimental study with a pre and post-test design with two experimental and control groups. The statistical population of this study included all nurses of Shahid Beheshti Hospital in Yasuj. In the present study, a random sampling method was used to determine the sample. 70 nurses were selected as a statistical sample. From this number, couples and subjects were replaced in two experimental and control groups (35 subjects in each group). Rif psychological well-being questionnaire and standard social capital questionnaire were used to collect data. Data were analyzed using analysis of covariance statistics. Results: The results displayed that the mean score of psychological well-being and psychological capital in the experimental group increased in the post-test stage compared to the pre-test. Moreover, the results of the covariance analysis test also indicated that the mean score of psychological capital, psychological well-being between before and after the intervention was statistically significant (P <0. 05). Conclusion: Group training of life skills could be effective in increasing psychological well-being, psychological capital and quality of life of nurses. It seems that life skills training can be used as an appropriate intervention method to promote the psychological capital and psychological well-being of nurses.