Purpose: To evaluate whether laser in situ keratomileusis (LASIK) is a safe and effective treatment for myopia, hyperopia and astigmatism after penetrating keratoplasty (PK).Setting: Medical University of Mashad; Toos LASIK clinic.Patients and Methods: Twelve eyes of 9 patients who had LASIK after PKP were reviewed (interventional case series). The interval between LASIK and PKP was at least 1 year, and mean follow-up after LASIK was 8 months. The Nidek EC 5000® excimer laser was used.Results: Nine patients (12 eyes) underwent LASIK following PK between 2000 and March 2002. Mean age of patients at the time of LASIK was 30.1 years (range, 22-37). Keratoconus was the indication for PK in the majority of the eyes (91.6%). Anisometropia was the indication for LASIK following PK. No intraoperative complications occurred. Six months after LASIK, mean best spectacle-corrected visual acuity (BSCVA) was 9/10 (in decimal scale) with no statistic difference from preoperative BSCVA (9/10) Mean spherical equivalent was reduced from -6.68 D to -0.38 D (95%) one day and to -1.34 D (80%) one year after LASIK. Mean refractive astigmatism was reduced from 6.5 D (mean attempted correction was 5.2 D) to 2.9 D one day 75% reduction (relative to attempted correction) and to 3.42 D one year 67% reduction (relative to attempted correction) after LASIK. Uncorrected visual acuity was 5/10 or better in all eyes after LASIK.Conclusion: LASIK is safe and effective in the treatment of myopia, hyperopia and astigmatism following PK.